The Mechanism of Sugar Signal Involved in Regulating Re-flowering of Tree Peony Under Forcing Culture

doi:10.16420/j.issn.0513-353x.2021-1250

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (3): 596-606.doi: 10.16420/j.issn.0513-353x.2021-1250

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The Mechanism of Sugar Signal Involved in Regulating Re-flowering of Tree Peony Under Forcing Culture

XUE Yuqian¹^,²^,^*, LIU Zhiyong²^,^*, SUN Kairong², ZHANG Xiuxin², LÜ Yingmin¹^,^**(), XUE Jingqi²^,^**()

¹College of Landscape Architecture，Beijing Forestry University，Beijing 100083，China
²Key Laboratory of Biology and Genetic Improvement of Horticultural Crops，Ministry of Agriculture and Rural Affairs，Institute of Vegetables and Flower，Chinese Academy of Agricultural Sciences，Beijing 100081，China

Received:2022-11-25 Revised:2023-01-13 Online:2023-03-25 Published:2023-04-03
Contact: **（E-mail：xuejingqi@caas.cn，luyingmin@bjfu.edu.cn）

Abstract

Abstract:

In this study，tree peony cultivar‘Luoyang Hong’was used as the material. Under the conditions of forcing culture，the flower buds were defoliated and treated with gibberellin to explore the effects on the development of flower buds，leaf stomatal characteristics，and glucose，fructose，sucrose，trehalose-6-phosphate（T6P）levels and the expression patterns of genes related to sugar signaling pathway. The results showed that both defoliation and gibberellin treatments can effectively promote the development of flower buds and can significantly promote the development of stomata and increase the opening of the stomata；at the same time，defoliation and gibberellin treatments could promote the metabolic consumption of energy substances，such as sucrose and glucose. The sugar signaling pathway was triggered by sensing the accumulation of sugar signaling molecule T6P content，and finally it promoted the development of flower bud by up-regulating the expression of PsTPS1 in leaves and inhibiting the expression of PsSnRK1 and PsHXK1.

Key words: tree peony, forcing culture, sugar signal, T6P, flowering regulation

CLC Number:

S685.11

XUE Yuqian, LIU Zhiyong, SUN Kairong, ZHANG Xiuxin, LÜ Yingmin, XUE Jingqi. The Mechanism of Sugar Signal Involved in Regulating Re-flowering of Tree Peony Under Forcing Culture[J]. Acta Horticulturae Sinica, 2023, 50(3): 596-606.

Figures/Tables 7

Table 1 Primers sequences of sugar signaling-related genes for expression analysis

基因名称 Gene name	引物名称 Primer name	引物序列（5′-3′） Sequence	产物长度/bp Length of product
PsTPS1	qRTTPS1	F：CTCTCGACTTCCATGCCTTA；R：CTGTGATGTTCCAAGGGTTC	207
PsSnRK1	qRTSnRK1	F：CACGAAGGAATGGCGAATA；R：CAAGGAAGGCAGCACAAAG	211
PsHXK1	qRTHXK1	F：ATAAGAAAAGCCGTGGTAGAGC；R：AGTGTTCATACAAGCCACCATC	166
Actin	qRTActin	F：GAGAGATTCCGTTGCCCAG；R：TCCTTGCTCATTCTGTCTGC	191

Fig. 1 Effect of defoliation and GA treatment on the development of bud and leaf of tree peony‘Luoyang Hong’

Fig. 2 SEM micrograph cross-sections of tree peony‘Luoyang Hong’leaf stomata under partial defoliation and GA treatment on the 15th day

Table 2 Characteristics of leaf stomata of tree peony‘Luoyang Hong’under partial defoliation and GA treatment on the 15th day

处理 Treatment	气孔开度/μm Stomatal aperture	气孔密度/（No · mm^-2） Stomatal density	气孔长度/μm Stomatal length	气孔宽度/μm Stomatal width	气孔面积/μm² Stomatal area
对照Control	0.27 ± 0.21 c	251.85 ± 26.91 a	28.68 ± 2.81 a	23.58 ± 1.96 b	501.44 ± 31.66 c
剥叶Partial defoliation	0.55 ± 0.20 b	237.04 ± 36.29 a	28.96 ± 1.95 a	24.92 ± 1.31 a	559.46 ± 23.42 b
赤霉素GA	0.98 ± 0.47 a	181.49 ± 21.85 b	28.80 ± 2.20 a	25.69 ± 2.11 a	593.61 ± 26.55 a

Fig. 3 The change of sucrose，glucose，fructose and T6P content at‘Luoyang Hong’tree peony under forcing culture Different letters indicate significant differences at P < 0.05 level，n = 3.

Fig. 4 Phylogenetic analysis of TPS1（A），SnRK1（B）and HXK1（C）amino acid in various plants The scale in represent the difference of every TPS1，SnRK1 and HXK1 amino acid from different plants.

Fig. 5 Effect of defoliation and GA treatment on the expression of PsTPS1，PsSnRK1，and PsHXK1 in leaves of tree peony‘Luoyang Hong’ The different lowercase letters indicated a significant difference among different treatment in expression of gene（Duncan’s test at P < 0.05 after analysis of variance；data are shown as mean ± SD，n = 3）.

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The Mechanism of Sugar Signal Involved in Regulating Re-flowering of Tree Peony Under Forcing Culture

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