Antifungal Mechanisms of Sodium New Houttuyfonate Against Penicillium digitatum

doi:10.16420/j.issn.0513-353x.2021-0982

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (1): 145-152.doi: 10.16420/j.issn.0513-353x.2021-0982

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Antifungal Mechanisms of Sodium New Houttuyfonate Against Penicillium digitatum

DU Yuling, YANG Fan, ZHAO Juan, LIU Shuqi, LONG Chaoan^*()

National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops，Key Laboratory of Horticultural Plant Biology Ministry of Education，National Citrus Preservation Technology R & D Center，College of Horticultural & Forestry Sciences，Huazhong Agricultural University，Wuhan 430070，China

Received:2022-08-26 Revised:2022-11-15 Online:2023-01-25 Published:2023-01-18
Contact: *（E-mail：calong@mail.hzau.edu.cn）

Abstract

Abstract:

In order to explore the antifungal mechanisms of Sodium New Houttuyfonate（SNH）against Penicillium digitatum，in vitro and in vivo experiments were conducted. The results showed that the minimum inhibitory concentration and minimum fungicidal concentration of SNH were 1 and 2 mmol · L^-1 respectively；with the increase of SNH concentration，the spore germination rate of P. digitatum decreased significantly and the relative conductivity increased significantly. The results of transmission electron microscope showed that 1 mmol · L^-1 treatment could destroy the cell structure of P. digitatum，resulting in the dissolution of cytoplasmic components，the disappearance of mitochondrial structure and the separation of plasma wall. At the same time，1 mmol · L^-1 SNH can inhibit the growth of a variety of citrus fungal diseases，showing broad-spectrum antifungal activity. When the fruits were artificially inoculated with 5 and 10 mmol · L^-1 SNH + Penicillium digitatum spore mixture，the lesion diameter of summer orange was significantly smaller than that of the control group. The above results showed that SNH could significantly inhibit the growth of P. digitatum，and had the prospect of control citrus green mold.

Key words: citrus, postharvest, green mold, biological control, Sodium New Houttuyfonate, Penicillium digitatum, antifungal mechanism

CLC Number:

S666

DU Yuling, YANG Fan, ZHAO Juan, LIU Shuqi, LONG Chaoan. Antifungal Mechanisms of Sodium New Houttuyfonate Against Penicillium digitatum[J]. Acta Horticulturae Sinica, 2023, 50(1): 145-152.

Figures/Tables 7

Fig. 1 Effect of SNH treatment on colony diameter of Penicillium digitatum Different lowercase letters represent significant difference at 0.05 level. The same below.

Fig. 2 Effect of SNH treatment on spore germination of Penicillium digitatum

Fig. 3 Effect of SNH on cell membrane permeability of Penicillium digitatum

Fig. 4 Transmission electron microscopy of mycelial morphology of Penicillium digitatum Arrows in B and E indicate cytoplasm，and in C indicates mitochondria.

Table 1 Inhibitory effect of SNH on a variety of Citrus fungal diseases

真菌 Fungus	抑制率/% Inhibition rate
指状青霉Penicillium digitatum（P44）	89.49 ± 6.94
扩展青霉Penicillium expansum（PTY-1）	100.00 ± 0.00
扩展青霉Penicillium expansum（L）	100.00 ± 0.00
酸腐病原菌 Geotrichum citri-aurantii（AY-1）	52.63 ± 3.21
意大利青霉Penicillium italicum（B3）	100.00 ± 0.00
意大利青霉Penicillium italicum（P6）	82.05 ± 20.58
炭疽病病原菌 Colletotrichum gloeosporioides	23.80 ± 15.55
产黄青霉Penicillium chrysogenum（B5）	100.00 ± 0.00
产黄青霉Penicillium chrysogenum（Q）	10.48 ± 1.55

Fig. 5 Effect of SNH on lesion diameter of green mold in citrus fruit

Fig. 6 Effect of SNH on green mold of summer orange fruit

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Antifungal Mechanisms of Sodium New Houttuyfonate Against Penicillium digitatum

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