Identification and Analysis of PAP1/2 Homologous Genes in Brassica rapa,B. oleracea and B. napus

doi:10.16420/j.issn.0513-353x.2021-0629

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (6): 1301-1312.doi: 10.16420/j.issn.0513-353x.2021-0629

• Research Papers • Previous Articles Next Articles

Identification and Analysis of PAP1/2 Homologous Genes in Brassica rapa,B. oleracea and B. napus

CHEN Daozong, LIU Yi, SHEN Wenjie, ZHU Bo, TAN Chen()

College of Life Sciences,Ganzhou Key Laboratory of Greenhouse Vegetable,Gannan Normal University,Ganzhou,Jiangxi 341000,China

Received:2021-06-29 Revised:2021-11-27 Online:2022-06-25 Published:2022-07-05
Contact: TAN Chen E-mail:tanchen2020@gnnu.edu.cn

Abstract

Abstract:

PAP1/2 transcription factors are the main MYB transcription factors involved in the formation of MBW complex to regulate anthocyanin biosynthesis. Using Arabidopsis PAP1/2 transcription factor（TF）nucleic acid and protein sequence alignment and Pfam verification to study the exact homologous copy number,evolutionary relationship and expression of PAP1/2 TFs regulating anthocyanin metabolism in Brassica plants such as Brassica rapa,B. oleracea and B. napus. The number of homologous copies of PAP1/2 TFs identified in B. rapa,B. oleracea and B. napus were three,four and nine,respectively. The evolutionary relationship between different copies of PAP1/2 TFs was further clarified by evolution analysis and microcollinearity analysis of the segment where the homologous copies of PAP1/2 TFs were located. The relationship between different copies of PAP1/2 TFs on chromosomes A7 and C6 was clarified. Finally,combined with the transcriptome sequencing data of different tissues of B. rapa,B. oleracea and B. napus,the expression analysis was carried out,and the expression of homologous copies of PAP1/2TFs in different tissues was clarified.

Key words: Brassica rapa, B. Oleracea, B. Napus, homologous gene, conserved domain, anthocyanin, R2R3-MYB transcription factor, PAP1/2

CLC Number:

S635

CHEN Daozong, LIU Yi, SHEN Wenjie, ZHU Bo, TAN Chen. Identification and Analysis of PAP1/2 Homologous Genes in Brassica rapa,B. oleracea and B. napus[J]. Acta Horticulturae Sinica, 2022, 49(6): 1301-1312.

Figures/Tables 10

References 31

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Function	拟南芥 A. thaliana	白菜 B. rapa	甘蓝 B. oleracea	甘蓝型油菜-AA B. napus-AA	甘蓝型油菜-CC B. napus-CC
PAP1(MYB75)	AT1G56650	BraA02g017040.3C	Bo00835s060.1	BnaA02G0160600ZS	BnaC02G0205500ZS
PAP1(MYB75)	AT1G56650	BraA03g040840.3C	Bo3g081880.1	BnaA03G0376600ZS	BnaC03G0461400ZS
PAP1(MYB75)	AT1G56650		Bo6g099880.1	BnaA07G0286900ZS	BnaC06G0328400ZS
PAP1(MYB75)	AT1G56650				BnaC06G0328700ZS
PAP2(MYB90)	AT1G66390	BraA07g032100.3C	Bo6g100940.1	BnaA07G0287000ZS	BnaC06G0329100ZS

Function	拟南芥 A. thaliana	白菜 B. rapa	甘蓝 B. oleracea	甘蓝型油菜-AA B. napus-AA	甘蓝型油菜-CC B. napus-CC
PAP1(MYB75)	AT1G56650	BraA02g017040.3C	Bo00835s060.1	BnaA02G0160600ZS	BnaC02G0205500ZS
PAP1(MYB75)	AT1G56650	BraA03g040840.3C	Bo3g081880.1	BnaA03G0376600ZS	BnaC03G0461400ZS
PAP1(MYB75)	AT1G56650		Bo6g099880.1	BnaA07G0286900ZS	BnaC06G0328400ZS
PAP1(MYB75)	AT1G56650				BnaC06G0328700ZS
PAP2(MYB90)	AT1G66390	BraA07g032100.3C	Bo6g100940.1	BnaA07G0287000ZS	BnaC06G0329100ZS

	拟南芥 A. thaliana	白菜B. rapa Chiifu-A2	甘蓝B. oleracea TO1000-C2	甘蓝型油菜B. napus ZS11-A2	甘蓝型油菜B. napus ZS11-C2
A2-C2	AT1G66330	BraA02g017010.3C	Bo2g052820.1	BnaA02G0160200ZS	BnaC02T0204500ZS
	AT1G66390	BraA02g017040.3C	Bo00835s060.1	BnaA02T0160600ZS	BnaC02T0205500ZS
	AT1G66470	BraA02g017080.3C	Bo00835s050.1	BnaA02T0160700ZS	BnaC02T0205700ZS
A3-C3	AT1G66330	Null	Null	Null	Null
	AT1G56650	BraA03g040840.3C	Bo3g081880.1	BnaA03G0376600ZS	BnaC03G0461400ZS
	AT1G66470	Null	Null	Null	Null
A7-C6	AT1G66210	BraA07g032000.3C	Bo6g099780.1	BnaA07T0285500ZS	BnaC06T0327300ZS
	AT1G66390	BraA07g032100.3C	Bo6g099880.1	BnaA07G0286900ZS	BnaC06G0328400ZS
	AT1G66390	BraA07g032100.3C	Bo6g099880.1	BnaA07G0286900ZS	BnaC06G0328700ZS
	AT1G66390	BraA07g032100.3C	Bo6g099880.1	BnaA07G0287000ZS	BnaC06G0329100ZS
	AT1G66840	BraA07g032410.3C	Bo6g101320.1	BnaA07T0289800ZS	BnaC06T0333000ZS

	拟南芥 A. thaliana	白菜B. rapa Chiifu-A2	甘蓝B. oleracea TO1000-C2	甘蓝型油菜B. napus ZS11-A2	甘蓝型油菜B. napus ZS11-C2
A2-C2	AT1G66330	BraA02g017010.3C	Bo2g052820.1	BnaA02G0160200ZS	BnaC02T0204500ZS
	AT1G66390	BraA02g017040.3C	Bo00835s060.1	BnaA02T0160600ZS	BnaC02T0205500ZS
	AT1G66470	BraA02g017080.3C	Bo00835s050.1	BnaA02T0160700ZS	BnaC02T0205700ZS
A3-C3	AT1G66330	Null	Null	Null	Null
	AT1G56650	BraA03g040840.3C	Bo3g081880.1	BnaA03G0376600ZS	BnaC03G0461400ZS
	AT1G66470	Null	Null	Null	Null
A7-C6	AT1G66210	BraA07g032000.3C	Bo6g099780.1	BnaA07T0285500ZS	BnaC06T0327300ZS
	AT1G66390	BraA07g032100.3C	Bo6g099880.1	BnaA07G0286900ZS	BnaC06G0328400ZS
	AT1G66390	BraA07g032100.3C	Bo6g099880.1	BnaA07G0286900ZS	BnaC06G0328700ZS
	AT1G66390	BraA07g032100.3C	Bo6g099880.1	BnaA07G0287000ZS	BnaC06G0329100ZS
	AT1G66840	BraA07g032410.3C	Bo6g101320.1	BnaA07T0289800ZS	BnaC06T0333000ZS

保守结构域名称 Motif name	保守结构域序列 Motif sequence	e值 e-value	宽度 Width
Motif 1	C[KN]T[KQ]M[KR]KRN[IV][PT][CF]S[SY]T[TQ]PAQK[IN][DE]V[FL]KPRPR[SL] FTVNNGCSH[LIF][NH]G[ML]P[EK][VA]D[VI][VI]P	1.4e-672	50
Motif 2	[YS]ELV[SN]NLMDG[EQ]N[MR]WW[EK]SLL[ED]ES[QK][DEQ]P[AD][AG][LI][VF] P[EK][AGS]T[AE]T[KE]K[GL]AT[SF]AFDVEQLW[SN]	3.1e-601	50
Motif 3	AWTAEED[SN]LLR[QR]CIDKYGEGKWHQVPLRAGL	8.7e-512	31
Motif 4	K[LF][SN]SDEVDLLLRLHKLLGNRWSLIAGRLPGRTAND[VI]KNYWNTHLSKKHEP	7.6e-799	50
Motif 5	RCRKSCRLRWLNYLKPSI[KN][RK]G	3.0e-340	21
Motif 6	[ND][TIA]NNV[CS]EN[SI][IFM]T[CY][NK][KN]D	2.0e-156	15
Motif 7	ME[GD]S[SP]KGL[RKT]KG	1.2e-113	11
Motif 8	[LM]LDGET[VG][ET]	7.2e-62	8
Motif 9	[LP][CF]LG[LFV]N	1.4e-20	6

Identification and Analysis of PAP1/2 Homologous Genes in Brassica rapa,B. oleracea and B. napus

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