Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (3): 597-612.doi: 10.16420/j.issn.0513-353x.2020-0942

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Genome-wide Identification and Expression Analysis of GDSL Esterase/Lipase Genes in Dimocarpus longan

LIU Mengyu, JIANG Mengqi, CHEN Yan, ZHANG Shuting, XUE Xiaodong, XIAO Xuechen, LAI Zhongxiong, LIN Yuling*()   

  1. Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou 350002,China
  • Received:2021-05-31 Revised:2021-09-09 Online:2022-03-25 Published:2022-03-25
  • Contact: LIN Yuling


The evolution characteristics of the longan GDSLDlGDSL)family and their expression patterns during the early stages of somatic embryogenesis were investigated. The results showed the 118 members of DlGDSL family can be divided into 15 subfamilies,and the subcellular localization was mainly distributed in the extracell,a few distributed in other parts such as cell membrane,chloroplast,mitochondria and endoplasmic reticulum. Analysis of chromosomal locations suggests that 111 out of 118 DlGDSLs are distributed unevenly on the 15 longan chromosomes. Analysis of synteny relationships indicates 13 pairs of DlGDSL genes. The genomic structures of those DlGDSL genes indicates that their exon numbers vary between two and twelve. The protein conserved motifs of those DlGDSL suggest motif2 and motif3 are particularly important. The cis-acting elements of DlGDSL promoters contained light responsive elements,hormone response,anaerobic induction,stress responsive and circadian control elements. Besides,three family members(A,B and C)of DlGDSL contains a large number of methyl jasmonate(MeJA),abscisic acid(ABA),auxin(IAA)and gibberellin(GA)action elements. In addition,transcriptome data analysis revealed that 26 differential expression genes from embryogenic callus to globular embryo stage,suggesting members of the DlGDSL family may play important roles in the morphogenesis of longan somatic embryos. The results of qRT-PCR analysis in different hormone treatments suggests some members of the DlGDSL family may be related to hormone signal transduction such as ABA and MeJA in longan embryogenic callus. DlGDSL57/69/105 genes showed obvious up-regulation and DlGDSL23/42/50/52/75/80/104 genes demonstrated visible down-regulation in ABA treatment. DlGDSL100 gene revealed obvious up-regulation and DlGDSL23/27/42/50/52/57/69/80/104/105 showed visible down-regulation in MeJA treatment. In summary,the research will provide a reference for the subsequent functional verification of DlGDSL genes in somatic embryogenesis.

Key words: Dimocarpus longan, GDSL, genome wide, indentification, expression analysis

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