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Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (6): 1053-1066.doi: 10.16420/j.issn.0513-353x.2020-0653

• Research Papers •     Next Articles

Analysis and Identification of circRNAs of Hazel Ovule at Different Developmental Stages

LIU Jianfeng, SUN Ying, WEI Heng, HE Hongli, ZHANG Xingzheng, CHENG Yunqing()   

  1. Jilin Provincial Key Laboratory of Plant Resource Science and Green Production,Jilin Normal University,Siping,Jilin 136000,China
  • Received:2021-02-07 Revised:2021-05-11 Online:2021-06-25 Published:2021-07-07
  • Contact: CHENG Yunqing E-mail:Chengyunqing1977@163.com

Abstract:

The differentially expressed circRNA(DEcircRNA)of hazel ovule were screened at different developmental stages,and their target differentially expressed mRNA(DEmRNA)was further identified to clarify the regulatory effect of circRNAs on ovule development. Samples were collected from ovule formation stage(Ov1),early growth stage(Ov2),rapid growth stage(Ov3)and maturation stage(Ov4)of hazel. The DEcircRNA involved in ovule development was identified based on the available results of mRNA sequencing analysis at four developmental stages. A total of 7 122 circRNAs were identified,and 59.54% of them came from exon region. In pairwise comparisons of Ov1-vs-Ov2,Ov1-vs-Ov3,Ov1-vs-Ov4,Ov2-vs-Ov3,Ov2-vs-Ov4 and Ov3-vs-Ov4,123,162,187,85,105 and 21 DEcircRNAs were identified respectively. The expression pattern of DEcircRNAs was developmental stage specific. Correlation analysis showed that 199 target mRNAs of DEcircRNA were also differentially expressed. The expression changes of DEcircRNA and their responding DEmRNA were highly synchronized. The results of GO(Gene Ontology)classification indicated that DEcircRNAs were involved in the regulation of metabolism during ovule development. Four DEcircRNAs and their target differentially expressed mRNAs at four ovule development stages were randomly selected and subjected to qRT-PCR analysis,and their expression levels changes were consistent with the results of high-throughput sequencing of microRNAs.

Key words: hazel, transcriptome, ovule, circular RNA

CLC Number: