The Functional Analysis of RcTCP9 Gene in Response to Salt and Drought Stress in Rosa chinensis‘Yueyuehong’

doi:10.16420/j.issn.0513-353x.2024-0791

Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (7): 1789-1802.doi: 10.16420/j.issn.0513-353x.2024-0791

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles

The Functional Analysis of RcTCP9 Gene in Response to Salt and Drought Stress in Rosa chinensis‘Yueyuehong’

DONG Xuanke¹^,², LI Yingyang¹^,², MA Yuwan¹^,², CAI Yanfang¹, DING Qihan¹^,², CHEN Haixia¹^,², LI Yufan¹^,²^,^*(), and CHEN Jiren¹^,²^,^*()

¹ College of Horticulture，Hunan Agricultural University，Changsha 410128，China
² Hunan Mid-subtropical Quality Plant Breeding and Utilization Engineering Technology Research Center，Changsha 410128，China

Received:2025-05-20 Revised:2025-05-07 Online:2025-07-23 Published:2025-07-23
Contact: LI Yufan, and CHEN Jiren

Abstract

Abstract:

Using cuttings of Rosa chinensis‘Yueyuehong’as materials，the expression patterns were analyzed of the RcTCP9 gene under varying durations of salt and drought stress. Results indicated that the expression of RcTCP9 was significantly upregulated under both stress conditions，demonstrating its active response. The gene was transiently expressed in tobacco，and observations of green fluorescence signals revealed that the encoded protein localized to the nucleus. Using Virus-Induced Gene Silencing（VIGS）and transient overexpression experiments，silencing RcTCP9 led to physiological damage in plants under salt and drought stress，characterized by wilting of leaves and roots，increased ion permeability，and elevated malondialdehyde（MDA）levels，while the activities of catalase（CAT）and peroxidase（POD）decreased. Overexpressed rose petals exhibited reduced cellular membrane damage，lower fading rates，and decreased MDA levels，showing improved resistance. The expression levels of stress tolerance related genes in overexpressed plants under salt and drought stress were determined，and the results showed that the expression of RcTCP9 may enhance plant stress tolerance by regulating the RcPR4-1 and RcNAC091 related pathways. In summary，the RcTCP9 gene plays a significant role in enhancing the tolerance of roses to salt and drought stress.

Key words: rosa, RcTCP9, salt tolerance, drought tolerance, antioxidant

DONG Xuanke, LI Yingyang, MA Yuwan, CAI Yanfang, DING Qihan, CHEN Haixia, LI Yufan, and CHEN Jiren. The Functional Analysis of RcTCP9 Gene in Response to Salt and Drought Stress in Rosa chinensis‘Yueyuehong’[J]. Acta Horticulturae Sinica, 2025, 52(7): 1789-1802.

Figures/Tables 12

Table 1 The sequences of primers used for experiment

用途 Purpose	名称 Name	引物序列（5′-3′） Primer sequence
克隆RcTCP9基因CDS全长 Clone the full-length CDS of the RcTCP9 gene	KL-RcTCP9-F	ATGACGTCGTATTTTGAGGATCAAG
	KL-RcTCP9-R	TCACTGGCTTCCTGGGCCTG
亚细胞定位载体构建 Construction of subcellular localization vector	pBI121-RcTCP9-F	ACGGGGGACTCTAGAGGATCCATGACGTCGTATTTTGAGGA
亚细胞定位载体构建 Construction of subcellular localization vector	pBI121-RcTCP9-R	GGACTGACCACCCGGGGATCCCTGGCTTCCTGGGCCTGCGG
瞬时过表达载体构建 Construction of transient overexpression vector	1305-RcTCP9-F	GGAGAGAACACGGGGGACATGACGTCGTATTTTGAGGATCA
瞬时过表达载体构建 Construction of transient overexpression vector	1305-RcTCP9-R	CATCATGGTCTTTGTAGTCCTGGCTTCCTGGGCCTGCGGCGGG
VIGS基因沉默载体构建 Construction of VIGS gene silencing vector	TRV2-RcTCP9-F	CTGTGAGTAAGGTTACCGAATTCGTTCGTGGGGAACGGCGGAG
VIGS基因沉默载体构建 Construction of VIGS gene silencing vector	TRV2-RcTCP9-R	CGCGTGAGCTCGGTACCGGATCCCTTCCTGGGCCTGCGGCGG
实时荧光定量表达（qRT-PCR）	RcTCP9-F	TGGTAGTGGTAACAGTAACA
	RcTCP9-R	CTCCTGCTTATCGTAAATCTC
	RcCTP-F	GGGTGATGATGCAGCTTT
	RcCTP-R	TTAGCACTTGACCTCCTTCA
	RcPR4/1-F	ATGGCCGGAAAACAATGC
	RcPR4/1-R	GGGCTTGTCGGCATCC
	RcNAC091-F	TGTCTTCCTCGGAGTTACAGTTACC
	RcNAC091-R	CCCAGGGGTCGTATTTGTACAG

Fig. 1 The cloning of the CDS sequence of RcTCP9

Fig. 2 Construction of the TRV2-RcTCP9 silencing vector A：Comparison results between the effective fragment of RcTCP9 and TRV2-RcTCP9；B：Gel electrophoresis image of colony PCR

Fig. 3 Phylogenetic tree of RcTCP9 from Rosa chinensis and TCP proteins of other species

Fig. 4 The subcellular localization of RcTCP9 in tobacco leaves

Fig. 5 Tissue-specific expression analysis of RcTCP9 in cuttings of Rosa chinensis‘Yueyuehong’under salt and drought stress *P < 0.05，**P < 0.01. The same below

Fig. 6 The changes of phenotype phenotype，RcTCP9 expression and physiological indexes of roses cutting seedlings in VIGS silencing RcTCP9 group and control group under salt stress

Fig. 7 Antioxidant enzyme activity related indexes of roses under salt stress

Fig. 8 Overexpression of RcTCP9 enhanced the salt tolerance of roses EV：Empty vector；OE：Overexpression

Fig. 9 The changes of phenotype，RcTCP9 expression and physiological indexes of roses cuttings in VIGS silencing RcTCP9 group and control group under drought stress

Fig. 10 Antioxidant enzyme activity related indexes of rose under drought stress

Fig. 11 Overexpression of RcTCP9 enhanced the drought tolerance of roses

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The Functional Analysis of RcTCP9 Gene in Response to Salt and Drought Stress in Rosa chinensis‘Yueyuehong’

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