Drought Tolerance Improvement of Processing Tomato by Molecular Marker Assisted Selection

doi:10.16420/j.issn.0513-353x.2022-0695

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (7): 1429-1443.doi: 10.16420/j.issn.0513-353x.2022-0695

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles

Drought Tolerance Improvement of Processing Tomato by Molecular Marker Assisted Selection

SHEN Xinyan¹, HOU Xiaolei¹, SUN Peinan¹, LIU Minmin¹, TANG Yaping¹^,², LI Ning², LU Yong’en¹, YE Zhibiao¹, OUYANG Bo¹^,^*()

¹ National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops，College of Horticulture & Forestry Sciences，Huazhong Agricultural University，Wuhan 430070，China
² Institute of Horticultural Crops，Xinjiang Academy of Agricultural Sciences，Urumchi 830091，China

Received:2022-08-17 Revised:2023-01-29 Online:2023-07-25 Published:2023-07-26
Contact: OUYANG Bo

Abstract

Abstract:

Molecular marker-assisted selection was used to improve the drought tolerance of two processing tomato lines（Rigel 87-5 and M3）. In this breeding process，a drought-tolerant tomato line（M1040）was used as the donor，while Rigel 87-5 and M3 were used as the recurrent parents. The drought-tolerant molecular marker ID68 was used to select the backcrossing materials for four backcross generations. The drought tolerance of the improved lines（Rigel 87-5D and M3D）at seedling stage was evaluated in detail. The results showed that the survival rate of 87-5D and M3D was significantly higher than that of the recurrent parent after severe drought and rehydration，which can also be reflected by the curve of injury index. The growth，physiological and biochemical indicators under moderate drought stress showed that the improved lines were clearly better than the recurrent parents. Comparison of agronomic traits suggested that the genetic background of the improved lines was restored.

Key words: tomato, molecular breeding, backcross, drought tolerance, donor

SHEN Xinyan, HOU Xiaolei, SUN Peinan, LIU Minmin, TANG Yaping, LI Ning, LU Yong’en, YE Zhibiao, OUYANG Bo. Drought Tolerance Improvement of Processing Tomato by Molecular Marker Assisted Selection[J]. Acta Horticulturae Sinica, 2023, 50(7): 1429-1443.

Figures/Tables 8

Table 1 Polymorphic molecular markers

名称 Name	引物序列（5′-3′） Sequence	物理位置/bp Physical position
ID20	F：AGAGGCCGCATGTAACGACG；R：CGAGTCTCAGACGAAAGGTGAAAG	47 955 901
ID28	F：ATATCGGGGTCAGGTGGTACTATG；R：TGGTACTGCCCTCCTTTTGTTCT	49 922 901
ID32	F：ACTTCCGTCTCCCTAACAATCACT；R：ACAGAGCAACTCTCAACACCCAG	50 920 601
ID38	F：CATACAATGTCACTCCACACGACC；R：TTCTGAACCCAAGTTCCAAAGG	51 943 201
ID41	F：GATGACGGCTGCTGCTGAAC；R：TCTTGCTGCTACCATTGGGAC	52 936 401
ID59	F：TAACAGCTCTTTGAGGCGTAAGTG；R：CCGTCTTCGGATGTATTTGAATC	48 185 801
ID68	F：GTCAAACCAGACCCCTAACACC；R：GTACAACCCCAAGAACCACCAG	49 648 201
ID83	F：GTTTGATAGCCGAGCGAATTG；R：AACGTGTTTCGTGTTGTATGCC	53 854 001

Fig. 1 Backcross breeding of drought-tolerant processing tomato with molecular marker a：Schematic diagram of the drought-tolerant quantitative trait loci（QTL）and molecular marker position（Black bar represents the introgressed fragment from wild tomato species，and white bar represents the background chromosome of M82. Insertion/Deletion(InDel)markers are marked with ID followed by a number）. b：Backcross breeding technique roadmap（ID68 MAS stands for marker assisted selection with ID68）. c：Test result of recurrent parent 87-5 by ID68（M：5000 Plus marker. D，S and H represent the amplicon from drought-tolerant donor，recurrent parent 87-5 and heterozygous material，respectively）. d：Test result of BC3F1 generation（1-13）of the recurrent parent M3. e：Test result of BC4F2 generation（1-21）of M3. f：Test result of the improved line M3D by multiple InDel markers covering the QTLs.

Fig. 2 Drought injury index（A），survival rate after rehydration（B）and aboveground phenotype at 10 d（C）of tomato lines after drought treatment M1040：Drought-tolerant donor；M3 and 87-5：Recurrent parent；M3D and 87-5D：Improved lines. Data are mean ± SD. For survival rate，One-way analysis of variance（ANOVA）with Duncan’s multiple range test was performed after arcsine conversion of the original data. Different small letters on top of the bar indicate significant difference at P < 0.05.

Fig. 3 Relative growth of the improved processing tomato lines under moderate drought stress M1040：Drought-tolerant donor；M3 and 87-5：Recurrent parent；M3D and 87-5D：Improved lines. Relative plant height and stem diameter are expressed as the ratio of treatment to control under moderate drought stress. Data are mean ± SD. For relative leaf area，One-way analysis of variance（ANOVA）with Duncan’s multiple range test was performed after arcsine conversion of the original percentage data. Different small letters on top of the bar indicate significant difference at P < 0.05.

Fig. 4 Leaf relative water content（RWC），water use efficiency（WUE）and chlorophyll content of the improved processing tomato lines under moderate drought stress M1040：Drought-tolerant donor；M3 and 87-5：Recurrent parent；M3D and 87-5D：Improved lines.* indicates significant difference at α = 0.05 level using Student’s t-test.

Fig. 5 Photosynthetic parameters and chlorophyll fluorescence parameters of the improved processing tomato lines under moderate drought stress M1040：Drought-tolerant donor；M3 and 87-5：Recurrent parent；M3D and 87-5D：Improved lines. * and ** indicate significant difference at α = 0.05 and α = 0.01 level，respectively，using Student’s t-test.

Fig. 6 Differences of related biochemical indicators in the improved processing tomato lines under moderate drought stress M1040：Drought-tolerant donor；M3 and 87-5：Recurrent parent；M3D and 87-5D：Improved lines. * indicates significant difference at α = 0.05 level using Student’s t-test.

Table 2 Comparison of the agronomic traits between the improved processing tomato lines and recurrent parents

材料 Material	株高/cm Plant height	茎粗/mm Stem diameter	株幅/cm Plant width	复叶形状 Compound leaf shape	单果质量/g Single fruit weight
耐旱供体 M1040 Drought-tolerant donor M1040	133.87 ± 3.08 a	9.82 ± 0.09 c	60.41 ± 2.03 a	细叶 Narrow leaf	26.86 ± 5.10 c
改良系M3D Improved line M3D	112.68 ± 3.97 b	10.98 ± 0.17 a	47.32 ± 1.52 d	宽叶 Wide leaf	54.33 ± 5.87 ab
轮回亲本M3 Recurrent parent M3	117.82 ± 2.96 b	10.98 ± 0.10 a	49.27 ± 1.43 cd	宽叶 Wide leaf	52.11 ± 4.08 b
改良系87-5D Improved line 87-5D	104.01 ± 3.42 c	10.52 ± 0.11 b	52.95 ± 1.57 b	细叶 Narrow leaf	59.54 ± 4.67 a
轮回亲本87-5 Recurrent parent 87-5	100.98 ± 2.68 c	10.43 ± 0.19 b	51.51 ± 1.68 bc	细叶 Narrow leaf	55.75 ± 3.93 ab

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Drought Tolerance Improvement of Processing Tomato by Molecular Marker Assisted Selection

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