Identification and Development of SNP Markers for Lagerstroemia indica Leaf Color Based on Transcriptome and Genome Analysis

doi:10.16420/j.issn.0513-353x.2024-0650

Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (7): 1803-1816.doi: 10.16420/j.issn.0513-353x.2024-0650

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles

Identification and Development of SNP Markers for Lagerstroemia indica Leaf Color Based on Transcriptome and Genome Analysis

YU Cannan¹, GUO Anfang¹, LU Chun², MIAO Hongjun², HOU Xiaoyu¹, YUAN Tianyi¹, LIU Guoyuan¹, WEI Hui¹, ZHANG Jian¹, and YU Chunmei¹^,^*()

¹ Key Lab of Landscape Plant Genetics and Breeding of Nantong，School of Life Science，Nantong University，Nantong，Jiangsu 226019，China
² Nantong Greening and Landscape Gardening Development Co., Ltd.，Nantong，Jiangsu 226001，China

Received:2024-10-15 Revised:2025-04-18 Online:2025-07-23 Published:2025-07-23
Contact: and YU Chunmei

Abstract

Abstract:

To identify leaf color-linked SNP markers in crape myrtle cultivars（Lagerstroemia indica），19 crape myrtle cultivars and their F₁ progeny were utilized. This study conducted both eye-based and CIE L^*a^*b^*-based measurements of leaf color during various growth stages（young leaf stage，full leaf stage，full bloom stage，and leaf abscission stage），and integrated transcriptome and genome data with targeted sequencing to identify candidate SNP markers potentially linked to leaf color，and with these SNPs subsequently detected in the F₁ progeny using high-resolution melting curve（HRM）analysis. The results indicated that both eye-based estimation and CIE L^*a^*b^* values could effectively distinguish leaf colors，with the a^* value specifically differentiating green leaves from colored leaves（red and purple-red）. Additionally, significant differences were observed in brightness value L^*，yellowness value b^*，chroma C，and hue angle h among the three types of leaf color. A total of fifty-three candidate SNP sites were obtained through data mining，but targeted sequencing results revealed that only six of them had a ΔSNP greater than 0.2. Sanger sequencing and HRM analysis of the F₁ plants derived from‘Dynamite’and‘Baiyun Yingxia’showed that four SNPs had a correlation coefficient with leaf color ranging from 0.184 to 0.623 reaching a significant level，and one SNP marker exhibited dimorphism between green and colored leaves. The higher the frequency of the purple（red）（P）type genotype，the deeper the leaf color，suggesting that the purple-red leaf color is caused by the additive effect of multiple genes.

Key words: crape myrtle, leaf color, SNP molecular markers, data mining, molecular-assisted breeding

YU Cannan, GUO Anfang, LU Chun, MIAO Hongjun, HOU Xiaoyu, YUAN Tianyi, LIU Guoyuan, WEI Hui, ZHANG Jian, and YU Chunmei. Identification and Development of SNP Markers for Lagerstroemia indica Leaf Color Based on Transcriptome and Genome Analysis[J]. Acta Horticulturae Sinica, 2025, 52(7): 1803-1816.

Figures/Tables 11

References 28

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母本 Female	父本 Male	F₁群体数量 Plants of F₁ population	靶向测序材料数量/个 Plants for target sequencing	靶向测序材料编号 Code number of plants for target sequencing
Blush	白云映霞 Baiyun Yingxia	50	5	Z26 ~ Z30
Best red	白云映霞 Baiyun Yingxia	101	3	Z1 ~ Z3
Crimson Red	10-6	42	1	Z51
Crimson Red	白云映霞 Baiyun Yingxia	92	17	Z43 ~ Z50，Z52 ~ Z60
Crimson Red	10-16	51	12	Z31 ~ Z42
红火球 Dynamite	紫玉 Ziyu	35	3	Z23 ~ Z25
红火球 Dynamite	10-6	45	6	Z17 ~ Z22
红火球 Dynamite	白云映霞 Baiyun Yingxia	191	13	Z4 ~ Z16

母本 Female	父本 Male	F₁群体数量 Plants of F₁ population	靶向测序材料数量/个 Plants for target sequencing	靶向测序材料编号 Code number of plants for target sequencing
Blush	白云映霞 Baiyun Yingxia	50	5	Z26 ~ Z30
Best red	白云映霞 Baiyun Yingxia	101	3	Z1 ~ Z3
Crimson Red	10-6	42	1	Z51
Crimson Red	白云映霞 Baiyun Yingxia	92	17	Z43 ~ Z50，Z52 ~ Z60
Crimson Red	10-16	51	12	Z31 ~ Z42
红火球 Dynamite	紫玉 Ziyu	35	3	Z23 ~ Z25
红火球 Dynamite	10-6	45	6	Z17 ~ Z22
红火球 Dynamite	白云映霞 Baiyun Yingxia	191	13	Z4 ~ Z16

名称 Name	正向引物（5′-3′） Forward primer	反向引物（5′-3′） Reverse primer	退火温度/℃ T_m
PA01	TTTCGGATGTTAGAATAAGTCAATAGAGCAAG	CTTATTCCCAACACAAATGACACTAATAGAAA	61
PA02	ACACAGTTTTCAGCATCCTCTCATAATAAAAT	GTGCACATAATTGTCATTAGATTCATTTGGTG	62
PA03	CTCCACCTCCACCTTACTACTACTCATC	CATCTGTAGCAATAAACCTTCCCAACTAC	62
PA04	GTCTCCTCCTGGCTCTCTTCCTTG	AGTAGTTTTATAGAATCCAGCTCATGCATTTC	62
PA05	AAAAATATTTAAGGAAAGAAGCAGACAAAGCC	ACACTTCACCAATTCAATCTCCATATATGTCT	62
PA06	ATGGAGGGAGTGGTAATTTTGGAATAAGTAAT	GGATTTTTCTTTTCATGTTATTTTGCAGGGAT	62
PA07	AATTCTCTGTTTTCCCTAAAGAAAGAGGCTAA	TAAAACTTCTGATAAATCCACCAGAGAAACGA	62
PA08	CCGTGTAGTTTATCCACAACATTATTCAGT	TTAGTTAAAACAAGAGAGAGAGACAGAGAGAG	62
PA09	AGCTGATCTGTATCAATAAATAGCAACAACTT	TTGATAGAATTTTCAGAATTACCCTTCCCATC	62
PA10	TCTTCTAACTTGTTTGGTTGATTTCTTGTTTG	GAATCATTTCGACACACACAATCATCATACTT	62
PA11	CTATCTTGAAGCCCCCAGTGACG	CTACAGGATCTCCCAGTCCGATGTG	63
PA12	TTAAAATTGTTCAATGCTAAAGTGCCTTATCG	CAATGAGAAGCTGAGTGTTTTCGATCTTAATG	62
PA13	CTTGCTTTACAGCAGCTAACATAAACTTCTAC	ATTATGGTAACATTCTCATGTTCGTACAGTTC	62
PA14	CTCCGAAAAGGTAAAGAAAGAAACAGTACGAT	ATTGTAAGCTTAGAGCTGCTTCACAATC	62
PA15	ATTAAATGAAAATGAGACGAAAGACATACCGT	CTGAAGAAGATCATCTACGCCGTCC	62
PA16	CCCCTCTATATATCTATCTCTCTGACTCATCA	GTAAAGGTTGTGTCAGTGTTGTTTCTGATTTG	62
PA17	CCACATTCCACATTACAAAATCAACCATTTTA	TTTTTCTTGTTCTTCAGTGTTCTTCAGTCG	62
PA18	GAAGGTGAGATCAATGAAATGTAGGGATAAAA	ATATTTTCATTTGGTGCAAATACATCACTTGT	62
PA19	ACTTGCCTGCTCTTTTTGTTTATTTTATCAAT	GTGTACTAATCAACTAATACCCAGAATGGTTC	62
PA20	ACTTGACGTTAGTAAACTACTTCTATCCATTG	AGCTTTTCAAGCTTAAATATGAGCATTAGTTG	62
PA21	AGAACCACTAAGTAATCATCGCAGTACTATAA	TGGGTGATATTGTTTCTACTTATTTTCCAGTT	62
PA22	TGTGTACAAGTTCTTAGGATACGTCAAAAA	TTCATAAACTGAGACGGATATTATGATTGGAG	62
PA23	ATGTCTTCCTGTGAATTTATTGGTTGTATTCT	GTATTGGCATTCAAGGACTGAATTTTGTAACT	62
PA24	TTTATCGGATATTATTCAAGCTTTACACTGCT	CTATAAGATGCTTTACTGTGGTCAAAAGACTG	62
PA25	TATCAAGTTCAAAATTATGCAAGCAGATGGTA	AATTACCGTCAATTGGATAGAGCCTACAGTTA	62
PA26	GGTATCACAGGAGAATATTACTGCACATCT	AGGATGATCTTAAAATTTCTCGTCTTCTTCTG	62
PA27	AGAATCATCCCTTTTTATAATTTTCTGGGCTT	TAATTGCATGGTTCAGATCAATTTCCATTAAC	62
PA28	CAGCAAGAGTGCCTTCCCATATATGTTT	CATAAAACCAAGCATTCTTCAACTCCCTTTTG	63
PA29	AGGATCATACCAACGTTCAAGATATTCAAAAA	GAATGGAGGGACAGGGATCTAGAAATAGAAAA	62
PA30	AAGAATCATCTAAAGGAGGGAATAGTATTGGG	ATGAAGAAGTGTTTCTTGATCTTGTGCG	62
PA31	GATGATATAGTTGATCAAATGAGGTTCGTGAT	TAGGAAATAGGGTTTTGGTACATTCAAACATT	62
PA32	CCCTTTGACAGGAGACAAGACTAAGAAAG	GATCCCTTTCTGCAAAGAAGGTTCCTG	62
PA33	AAATTTGTACTTTGAACTTCTTAGATTGTGCC	CACATGGATCAAAAGAATGATTCAAGATGGTC	63
PA34	GATAGAAACTCTATTTCCACTCAAAACCATGT	GCAAGTTAACGTTTATTTTGGGAAATTCTACT	62
PA35	AACCCATCTGATAAAGAAAACAATAGATGGAC	CTGTGTGTATATAAATACGTAAGGAGCTCAAG	62
PA36	CTAAGCATCTAGGCATGCATTTGAGATTT	AATTTGCATGACTCAAGAGATACCATAAAAGT	62
PA37	CTATTACCATTTGACCGTTGGTAAGGAGAG	TTTGATTCTGTTTAATTGGGTGATATGGAACT	63
PA38	CTGCGCCTTCTCAGAGGTTAAGAG	GATATGCAATTGCTAGAAAGTGAGGAAATCG	63
PA39	GGAACCATATATGTTGCACTCGATCTTTTATT	GAAGTGAAGGGATCGAACATCAATCAAACT	63
PA40	GATGGAAGATTTGGAGTAGGAGATGGAAGA	ACACTCTAAAAGGAAGAGCTATATTAGGAGTT	62
PA41	AATTAAAACCAGATTGGCTCATTACACATAGT	TTAGTGATGGGTTTTCTTTCCTTTTCAATTTC	62
PA42	AAAAAGATGTTCCATGAGAGAAAGTGATTCG	TGAGAGAGAAAGGAATTAGCAGTAAACTATGA	62
PA43	GATCGACAGAAAAGCTTCATGGTATTACTTAT	AACAAAATTGACTTGGTATTGCTATTTAAGCG	62
PA44	AAGAACTCGACATTCTCCAAGGAAAACAG	GAACACCAAATTCAACCCGAACAGATTC	63
PA45	TATCTCCTGTCCTAGGAATCAACTGAAATAAG	AGAAAAAGACAAATCCAACGGCTACATAAC	63
PA46	CTTTGCGCCTAACCTTAACTAGCATC	GTCCGTTGTCCAGTAGGATATAAACAAAATAG	62
PA47	CTCTTCTTCTTCTTCTTCTTCTTCTTCTTCAC	TAATTCTCCGTCTAGCTCCTTCGACATTT	62
PA48	CATGTTTTTCCAACCACCTTTTTGTTTTTG	CCATCTGTCTCTCTCATTCTTAGTTTCAATTC	62
PA49	TTATTCTTGCCAGATCTAGTGACTATCTCATC	TTTTCTGTGTATCTATATATTGTTCAGCTCGG	62
PA50	CATGTAGTAATTAACTTAACTCGGTGGGGATG	GATTGCTGATCTGCCGGGTCATT	63
PA51	GAGTTACCATCCAGTCCTAGCTCAC	GATTATCTTCGAGACGGACTGGACC	63
PA52	AAGAAGAAGCAGCCGAGGTAGAG	CCTTTCGTTAGTTCTTCTCCTCTCTGATTTAG	61
PA53	TCAATACAAAATCACATCTACCCAATGAAGAG	GTTGTGGTCATTGTGAGGGGATTGAG	63

名称 Name	正向引物（5′-3′） Forward primer	反向引物（5′-3′） Reverse primer	退火温度/℃ T_m
PA01	TTTCGGATGTTAGAATAAGTCAATAGAGCAAG	CTTATTCCCAACACAAATGACACTAATAGAAA	61
PA02	ACACAGTTTTCAGCATCCTCTCATAATAAAAT	GTGCACATAATTGTCATTAGATTCATTTGGTG	62
PA03	CTCCACCTCCACCTTACTACTACTCATC	CATCTGTAGCAATAAACCTTCCCAACTAC	62
PA04	GTCTCCTCCTGGCTCTCTTCCTTG	AGTAGTTTTATAGAATCCAGCTCATGCATTTC	62
PA05	AAAAATATTTAAGGAAAGAAGCAGACAAAGCC	ACACTTCACCAATTCAATCTCCATATATGTCT	62
PA06	ATGGAGGGAGTGGTAATTTTGGAATAAGTAAT	GGATTTTTCTTTTCATGTTATTTTGCAGGGAT	62
PA07	AATTCTCTGTTTTCCCTAAAGAAAGAGGCTAA	TAAAACTTCTGATAAATCCACCAGAGAAACGA	62
PA08	CCGTGTAGTTTATCCACAACATTATTCAGT	TTAGTTAAAACAAGAGAGAGAGACAGAGAGAG	62
PA09	AGCTGATCTGTATCAATAAATAGCAACAACTT	TTGATAGAATTTTCAGAATTACCCTTCCCATC	62
PA10	TCTTCTAACTTGTTTGGTTGATTTCTTGTTTG	GAATCATTTCGACACACACAATCATCATACTT	62
PA11	CTATCTTGAAGCCCCCAGTGACG	CTACAGGATCTCCCAGTCCGATGTG	63
PA12	TTAAAATTGTTCAATGCTAAAGTGCCTTATCG	CAATGAGAAGCTGAGTGTTTTCGATCTTAATG	62
PA13	CTTGCTTTACAGCAGCTAACATAAACTTCTAC	ATTATGGTAACATTCTCATGTTCGTACAGTTC	62
PA14	CTCCGAAAAGGTAAAGAAAGAAACAGTACGAT	ATTGTAAGCTTAGAGCTGCTTCACAATC	62
PA15	ATTAAATGAAAATGAGACGAAAGACATACCGT	CTGAAGAAGATCATCTACGCCGTCC	62
PA16	CCCCTCTATATATCTATCTCTCTGACTCATCA	GTAAAGGTTGTGTCAGTGTTGTTTCTGATTTG	62
PA17	CCACATTCCACATTACAAAATCAACCATTTTA	TTTTTCTTGTTCTTCAGTGTTCTTCAGTCG	62
PA18	GAAGGTGAGATCAATGAAATGTAGGGATAAAA	ATATTTTCATTTGGTGCAAATACATCACTTGT	62
PA19	ACTTGCCTGCTCTTTTTGTTTATTTTATCAAT	GTGTACTAATCAACTAATACCCAGAATGGTTC	62
PA20	ACTTGACGTTAGTAAACTACTTCTATCCATTG	AGCTTTTCAAGCTTAAATATGAGCATTAGTTG	62
PA21	AGAACCACTAAGTAATCATCGCAGTACTATAA	TGGGTGATATTGTTTCTACTTATTTTCCAGTT	62
PA22	TGTGTACAAGTTCTTAGGATACGTCAAAAA	TTCATAAACTGAGACGGATATTATGATTGGAG	62
PA23	ATGTCTTCCTGTGAATTTATTGGTTGTATTCT	GTATTGGCATTCAAGGACTGAATTTTGTAACT	62
PA24	TTTATCGGATATTATTCAAGCTTTACACTGCT	CTATAAGATGCTTTACTGTGGTCAAAAGACTG	62
PA25	TATCAAGTTCAAAATTATGCAAGCAGATGGTA	AATTACCGTCAATTGGATAGAGCCTACAGTTA	62
PA26	GGTATCACAGGAGAATATTACTGCACATCT	AGGATGATCTTAAAATTTCTCGTCTTCTTCTG	62
PA27	AGAATCATCCCTTTTTATAATTTTCTGGGCTT	TAATTGCATGGTTCAGATCAATTTCCATTAAC	62
PA28	CAGCAAGAGTGCCTTCCCATATATGTTT	CATAAAACCAAGCATTCTTCAACTCCCTTTTG	63
PA29	AGGATCATACCAACGTTCAAGATATTCAAAAA	GAATGGAGGGACAGGGATCTAGAAATAGAAAA	62
PA30	AAGAATCATCTAAAGGAGGGAATAGTATTGGG	ATGAAGAAGTGTTTCTTGATCTTGTGCG	62
PA31	GATGATATAGTTGATCAAATGAGGTTCGTGAT	TAGGAAATAGGGTTTTGGTACATTCAAACATT	62
PA32	CCCTTTGACAGGAGACAAGACTAAGAAAG	GATCCCTTTCTGCAAAGAAGGTTCCTG	62
PA33	AAATTTGTACTTTGAACTTCTTAGATTGTGCC	CACATGGATCAAAAGAATGATTCAAGATGGTC	63
PA34	GATAGAAACTCTATTTCCACTCAAAACCATGT	GCAAGTTAACGTTTATTTTGGGAAATTCTACT	62
PA35	AACCCATCTGATAAAGAAAACAATAGATGGAC	CTGTGTGTATATAAATACGTAAGGAGCTCAAG	62
PA36	CTAAGCATCTAGGCATGCATTTGAGATTT	AATTTGCATGACTCAAGAGATACCATAAAAGT	62
PA37	CTATTACCATTTGACCGTTGGTAAGGAGAG	TTTGATTCTGTTTAATTGGGTGATATGGAACT	63
PA38	CTGCGCCTTCTCAGAGGTTAAGAG	GATATGCAATTGCTAGAAAGTGAGGAAATCG	63
PA39	GGAACCATATATGTTGCACTCGATCTTTTATT	GAAGTGAAGGGATCGAACATCAATCAAACT	63
PA40	GATGGAAGATTTGGAGTAGGAGATGGAAGA	ACACTCTAAAAGGAAGAGCTATATTAGGAGTT	62
PA41	AATTAAAACCAGATTGGCTCATTACACATAGT	TTAGTGATGGGTTTTCTTTCCTTTTCAATTTC	62
PA42	AAAAAGATGTTCCATGAGAGAAAGTGATTCG	TGAGAGAGAAAGGAATTAGCAGTAAACTATGA	62
PA43	GATCGACAGAAAAGCTTCATGGTATTACTTAT	AACAAAATTGACTTGGTATTGCTATTTAAGCG	62
PA44	AAGAACTCGACATTCTCCAAGGAAAACAG	GAACACCAAATTCAACCCGAACAGATTC	63
PA45	TATCTCCTGTCCTAGGAATCAACTGAAATAAG	AGAAAAAGACAAATCCAACGGCTACATAAC	63
PA46	CTTTGCGCCTAACCTTAACTAGCATC	GTCCGTTGTCCAGTAGGATATAAACAAAATAG	62
PA47	CTCTTCTTCTTCTTCTTCTTCTTCTTCTTCAC	TAATTCTCCGTCTAGCTCCTTCGACATTT	62
PA48	CATGTTTTTCCAACCACCTTTTTGTTTTTG	CCATCTGTCTCTCTCATTCTTAGTTTCAATTC	62
PA49	TTATTCTTGCCAGATCTAGTGACTATCTCATC	TTTTCTGTGTATCTATATATTGTTCAGCTCGG	62
PA50	CATGTAGTAATTAACTTAACTCGGTGGGGATG	GATTGCTGATCTGCCGGGTCATT	63
PA51	GAGTTACCATCCAGTCCTAGCTCAC	GATTATCTTCGAGACGGACTGGACC	63
PA52	AAGAAGAAGCAGCCGAGGTAGAG	CCTTTCGTTAGTTCTTCTCCTCTCTGATTTAG	61
PA53	TCAATACAAAATCACATCTACCCAATGAAGAG	GTTGTGGTCATTGTGAGGGGATTGAG	63

品种 Cultivars	幼叶期 Young leaf stage	完全展叶期 Full leaf stage	盛花期 Full bloom stage	落叶期 Deciduous stage	季相分类 Classes
垂枝粉Chuizhifen	绿色Green	绿色Green	绿色Green	绿色Green	生长季绿色叶Green leaves during the growth season
紫锦Zijin	绿色Green	绿色Green	绿色Green	绿色Green	生长季绿色叶Green leaves during the growth season
紫玉Ziyu	绿色Green	绿色Green	绿色Green	绿色Green	生长季绿色叶Green leaves during the growth season
10-6	绿色Green	绿色Green	绿色Green	绿色Green	生长季绿色叶Green leaves during the growth season
白云映霞 Baiyun Yingxia	绿色Green	绿色Green	绿色Green	绿色Green	生长季绿色叶Green leaves during the growth season
10-16	绿色Green	绿色Green	绿色 Green	绿色Green	生长季绿色叶Green leaves during the growth season
NTU-1	紫红色 Red purple	绿色Green	绿色Green	绿色Green	春季彩叶Red purple leaves in spring
Blush V2	绿色Green	绿色Green	绿色Green	绿色Green	生长季绿色叶Green leaves during the growth season
Red Hot	红绿色 Red green	紫红色 Red purple	暗红色 Dark red	暗红色 Dark red	生长季彩叶Colored leaves during the growth season
Blush	紫色Purple	紫红色 Red purple	绿色Green	绿色Green	春夏两季相紫红叶Red purple leaves in spring and summer
Best Red	紫红色 Red purple	紫红色 Red purple	绿色Green	绿色Green	春夏两季相紫红叶Red purple leaves in spring and summer
Crimson Red	紫红色 Red purple	紫绿色 Purple green	绿色Green	绿色Green	春夏两季相紫红叶Red purple leaves in spring and summer
Pure White	绿色Green	紫红色 Red purple	绿色Green	红色Red	夏秋两季相彩叶Colored leaves in summer and autumn
澧浦2号 Lipu No. 2	黑紫色 Dark purple	黑紫色 Dark purple	黑紫色 Dark purple	黑紫色 Dark purple	生长季彩叶Colored leaves during the growth season
国旗红Guoqihong	紫红色 Red purple	亮红色 Britght red	绿色Green	绿色Green	春夏两季相紫红叶Red purple in spring and summer
红火球Dynamite	橙红色 Red orange	紫红色 Red purple	绿色Green	绿色Green	春夏两季相紫红叶Red purple in spring and summer
红火箭Red rocket	橙红色 Red orange	紫红色 Red purple	绿色Green	绿色Green	春夏两季相紫红叶 Red purple in spring and summer
天鹅绒 Pink Velour	紫红色 Red purple	紫红色 Red purple	绿色Green	绿色Green	春夏两季相紫红叶 Red purple in spring and summer
红叶乔木 Hongye Qiaomu	紫红色 Red purple	紫绿色 Purple green	绿色Green	绿色Green	春夏两季相紫红叶 Red purple in spring and summer

Identification and Development of SNP Markers for Lagerstroemia indica Leaf Color Based on Transcriptome and Genome Analysis

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编号	幼叶期 Young leaf stage			完全展叶期 Full leaf stage			色系分类
Code	L^*	a^*	b^*	L^*	a*	b^*	Classes
Z-4	34.41 ± 2.18	13.24 ± 1.22	8.48 ± 0.62	42.71 ± 2.97	-5.31 ± 1.35	24.53 ± 2.83	绿色 Green
Z-7	45.21 ± 2.26	8.56 ± 0.32	14.93 ± 0.21	43.99 ± 1.87	-1.65 ± 1.05	27.32 ± 2.69	绿色 Green
Z-8	34.90 ± 2.44	10.68 ± 1.30	9.37 ± 0.52	42.96 ± 2.48	-8.14 ± 0.79	26.73 ± 2.11	绿色 Green
Z-10	30.24 ± 2.84	10.85 ± 0.68	7.61 ± 1.10	42.98 ± 1.96	-6.93 ± 1.00	23.53 ± 0.61	绿色 Green
Z-18	35.30 ± 1.19	11.77 ± 1.13	12.50 ± 1.21	39.08 ± 1.60	-6.34 ± 1.58	21.49 ± 2.71	绿色 Green
Z-25	33.37 ± 2.38	9.72 ± 0.69	5.25 ± 1.27	43.82 ± 2.87	-3.61 ± 1.01	21.74 ± 2.61	绿色 Green
Z-31	34.26 ± 2.74	6.68 ± 0.42	8.44 ± 1.22	38.49 ± 3.52	-10.98 ± 1.80	21.35 ± 0.63	绿色 Green
Z-32	37.58 ± 2.66	10.47 ± 0.82	14.11 ± 0.97	39.80 ± 3.17	-8.89 ± 1.94	20.54 ± 1.01	绿色 Green
Z-33	37.42 ± 2.41	15.51 ± 1.43	9.65 ± 1.21	42.88 ± 2.88	-9.62 ± 1.46	24.51 ± 2.77	绿色 Green
Z-34	32.59 ± 2.66	14.00 ± 1.08	7.77 ± 1.01	37.09 ± 0.67	-9.11 ± 1.71	18.25 ± 2.06	绿色 Green
Z-35	36.95 ± 2.69	9.40 ± 0.83	13.41 ± 0.96	39.91 ± 2.23	-9.69 ± 1.89	24.30 ± 1.03	绿色 Green
Z-36	35.80 ± 2.53	12.58 ± 0.40	9.40 ± 0.86	41.50 ± 3.44	-11.23 ± 1.11	26.79 ± 2.37	绿色 Green
Z-37	34.29 ± 2.20	14.02 ± 1.05	6.31 ± 1.40	42.20 ± 1.74	-9.11 ± 1.43	24.19 ± 1.32	绿色 Green
Z-38	33.28 ± 2.07	13.14 ± 0.54	3.90 ± 0.43	41.60 ± 3.57	-8.07 ± 1.04	25.36 ± 0.59	绿色 Green
Z-39	31.97 ± 2.64	8.80 ± 0.82	4.28 ± 1.19	39.17 ± 3.43	-8.14 ± 1.22	16.31 ± 2.64	绿色 Green
Z-40	34.84 ± 2.20	9.49 ± 0.67	6.30 ± 0.77	38.85 ± 3.10	-8.87 ± 1.55	18.94 ± 0.68	绿色 Green
Z-41	41.04 ± 2.58	2.11 ± 0.54	19.15 ± 0.90	42.05 ± 2.77	-10.84 ± 0.7	26.76 ± 1.17	绿色 Green
Z-42	36.33 ± 3.24	8.89 ± 0.99	7.11 ± 0.53	36.95 ± 3.20	-8.46 ± 1.51	17.14 ± 2.97	绿色 Green
Z-44	37.99 ± 2.41	8.70 ± 1.08	10.21 ± 0.50	38.12 ± 1.78	-10.99 ± 1.42	15.30 ± 1.73	绿色 Green
Z-45	30.72 ± 2.68	10.17 ± 1.11	3.40 ± 0.83	39.09 ± 1.39	-7.18 ± 1.83	19.28 ± 2.27	绿色 Green
Z-46	34.60 ± 2.06	15.33 ± 0.40	10.91 ± 0.44	43.06 ± 1.60	-9.25 ± 0.48	26.70 ± 2.94	绿色 Green
Z-47	31.77 ± 2.66	10.54 ± 0.60	6.29 ± 1.14	38.03 ± 1.35	-8.43 ± 0.17	19.76 ± 2.97	绿色 Green
Z-48	32.56 ± 2.67	12.41 ± 1.38	5.19 ± 1.49	35.33 ± 2.66	-8.62 ± 0.35	16.89 ± 1.05	绿色 Green
Z-49	45.31 ± 2.84	2.41 ± 0.98	16.09 ± 0.64	38.53 ± 1.71	-9.64 ± 0.88	19.81 ± 1.14	绿色 Green
Z-50	32.36 ± 2.59	7.46 ± 0.58	9.20 ± 1.10	37.79 ± 3.11	-7.05 ± 1.41	14.86 ± 2.98	绿色 Green
Z-51	32.06 ± 2.44	11.63 ± 0.61	7.77 ± 0.82	36.94 ± 3.41	-7.14 ± 0.65	14.88 ± 2.17	绿色 Green
Z-52	27.96 ± 2.19	9.72 ± 0.68	3.52 ± 0.52	38.39 ± 1.02	-8.48 ± 1.48	18.14 ± 1.11	绿色 Green
Z-53	30.32 ± 2.68	7.42 ± 1.21	5.28 ± 1.47	37.69 ± 0.81	-6.46 ± 1.41	19.85 ± 2.02	绿色 Green
Z-54	33.91 ±2.13	12.00 ± 0.63	7.49 ± 1.30	43.06 ± 1.75	-4.97 ± 0.85	21.87 ± 1.93	绿色 Green
Z-55	32.50 ± 2.02	9.37 ± 1.23	7.51 ± 1.42	40.37 ± 1.16	-7.68 ± 0.49	21.23 ± 0.54	绿色 Green
Z-56	35.72 ± 2.40	12.06 ± 0.35	8.06 ± 0.86	47.22 ± 2.81	-11.49 ± 0.24	28.94 ± 2.88	绿色 Green
Z-57	31.87 ± 2.89	6.01 ± 0.81	6.57 ± 1.28	40.07 ± 3.88	-9.49 ± 1.31	22.61 ± 1.19	绿色 Green
Z-58	37.00 ± 2.25	6.93 ± 0.91	12.19 ± 0.53	41.51 ± 3.07	-10.55 ± 1.96	24.35 ± 1.90	绿色 Green
Z-59	39.88 ± 1 81	6.18 ± 0.63	16.45 ± 0.81	34.94 ± 1.69	-7.72 ± 0.35	16.26 ± 1.13	绿色 Green
Z-60	39.81 ± 2.02	9.99 ± 1.04	7.72 ± 0.46	45.10 ± 1.88	-9.76 ± 0.95	28.02 ± 2.94	绿色 Green
Z-2	33.35 ± 2.14	9.20 ± 0.59	13.78 ± 0.29	40.86 ± 1.90	2.02 ± 0.62	21.56 ± 2.88	红色 Red
Z-5	39.26 ± 1.77	14.14 ± 0.79	8.23 ± 1.48	35.41 ± 3.72	6.96 ± 0.62	11.60 ± 0.82	红色 Red
Z-6	36.06 ± 1.15	10.15 ± 0.85	8.38 ± 1.17	38.81 ± 2.81	3.89 ± 1.73	21.25 ± 2.94	红色 Red
Z-11	41.15 ± 1.97	11.95 ± 1.44	11.09 ± 0.82	35.45 ± 1.86	6.36 ± 1.94	14.58 ± 0.90	红色 Red
Z-12	43.73 ± 1.20	11.82 ± 0.74	13.07 ± 0.95	41.95 ± 2.88	6.47 ± 1.98	23.91 ± 1.15	红色 Red
Z-13	33.26 ± 1.18	13.33 ± 1.09	1.16 ± 0.36	38.23 ± 2.07	5.40 ± 1.27	15.24 ± 0.62	红色 Red
Z-14	44.59 ± 2.86	8.97 ± 1.01	10.24 ± 0.49	36.74 ± 2.22	4.67 ± 0.20	16.48 ± 2.25	红色 Red
Z-15	39.60 ± 2.92	14.15 ± 0.78	8.87 ± 0.23	37.47 ± 2.60	8.12 ± 1.91	18.86 ± 2.14	红色 Red
Z-16	40.50 ± 1.24	10.06 ± 0.82	6.69 ± 1.40	35.42 ± 2.75	8.56 ± 1.60	12.66 ± 1.61	红色 Red
Z-17	40.38 ± 1.86	15.19 ± 0.98	8.56 ± 1.47	38.00 ± 3.46	6.39 ± 0.21	15.78 ± 2.93	红色 Red
Z-24	44.55 ± 1.34	8.43 ± 0.88	10.36 ± 1.15	38.39 ± 2.99	8.70 ± 0.46	11.17 ± 2.22	红色 Red
Z-26	36.10 ± 2.92	4.37 ± 1.34	15.01 ± 1.4	42.20 ± 0.51	9.18 ± 1.10	11.68 ± 0.59	红色 Red
Z-1	28.20 ± 2.13	10.33 ± 0.91	6.81 ± 1.47	33.42 ± 1.49	12.98 ± 0.87	10.76 ± 1.77	紫红色 Red purple
Z-3	28.82 ± 2.43	7.70 ± 0.81	-0.03 ± 1.21	31.63 ± 0.93	5.91 ± 1.50	7.56 ± 2.50	紫红色 Red purple
Z-9	43.53 ± 2.71	8.41 ± 1.44	13.73 ± 0.72	32.12 ± 3.09	11.10 ± 0.76	10.20 ± 2.83	紫红色 Red purple
Z-19	32.32 ± 2.06	12.68 ± 1.22	5.33 ± 1.27	32.49 ± 3.08	10.89 ± 1.06	7.38 ± 2.84	紫红色 Red purple
Z-20	35.86 ± 2.20	7.90 ± 1.14	11.82 ± 0.53	33.56 ± 1.11	9.69 ± 1.22	7.68 ± 2.44	紫红色 Red purple
Z-21	33.74 ± 1.48	11.93 ± 0.73	3.07 ± 1.42	32.51 ± 1.42	10.57 ± 0.79	8.58 ± 2.85	紫红色 Red purple
Z-22	30.57 ± 2.31	9.92 ± 1.00	3.08 ± 1.47	32.02 ± 3.31	11.90 ± 1.30	10.15 ± 2.13	紫红色 Red purple
Z-23	27.93 ± 1.78	12.85 ± 0.70	1.71 ± 1.48	29.17 ± 3.81	6.10 ± 0.27	4.96 ± 0.59	紫红色 Red purple
Z-27	34.72 ± 2.92	4.15 ± 1.16	10.95 ± 1.35	34.15 ± 1.45	3.93 ± 0.21	2.42 ± 1.71	紫红色 Red purple
Z-28	30.89 ± 2.42	10.92 ± 1.35	4.26 ± 1.42	35.55 ± 1.38	2.71 ± 1.63	2.70 ± 1.89	紫红色 Red purple
Z-29	34.15 ± 1.04	15.12 ± 1.47	2.44 ± 0.67	34.34 ± 1.34	4.90 ± 0.51	1.12 ± 1.49	紫红色 Red purple
Z-30	38.11 ± 2.00	11.06 ± 1.44	5.93 ± 0.23	36.76 ± 1.93	8.80 ± 1.43	5.23 ± 2.63	紫红色 Red purple
Z-43	32.71 ± 1.22	2.79 ± 0.47	6.45 ± 1.39	30.29 ± 3.25	2.82 ± 0.92	5.04 ± 1.79	紫红色 Red purple

SNP标记 SNP markers	彩叶池SNP-index SNP-index of colored leaf pool	绿叶池SNP-index SNP-index of green leaf pool	∆SNP
V16	1.000	0.714	0.286
V18	0.875	0.500	0.375
V34	0.934	0.652	0.282
V35	0.656	0.429	0.227
V38	0.531	0.280	0.251
V40	0.969	0.714	0.255

名称 Name	正向引物（5′-3′） Forward primer	反向引物（5′-3′） Reverse primer
V16	ATCTTGAAGCCCCCAGTGAC	TGGCTTGGTGATGATGGGTG
V18	TTTTTGGTGTAAGCTCCCACT	CTCATGTTCGTACAGTTCAAC
V34	GCTGGCCAAGAATTGTTC	CGGAGGAGTAAAGTAACCAG
V35	CCCATCTTGTCACATGTGAG	CCGTAAGCCGATGAAAGA
V38	ATTGACTGGCGGACAACTGAG	CCATTGACAAGTAGATCCTTT

SNP名称 SNP name	PR型（红色） Purple type（red）（P）	Green型 Green type （G）	G型检测数 G type number	杂合基因检测数 Heterozygous number	P型检测数 P type number	r	显著水平 Significance
V16	C	CTG	37	12	41	0.623	**
V18	-	+	29	-	-	nd	-
V34	AT	A	25	20	45	0.184	*
V35	TCC	T	42	20	28	0.255	**
V38	G	GAC	32	26	32	0.618	**

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