Cloning of the RcRAP2.7 Gene and its Expression
Analysis Under Abiotic Stresses in Rosa
chinensis‘Yueyuehong’

doi:10.16420/j.issn.0513-353x.2024-0532

Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (4): 921-932.doi: 10.16420/j.issn.0513-353x.2024-0532

• Genetic & Breeding · Germplasm Resources · Molecular Biology • Previous Articles Next Articles

Cloning of the RcRAP2.7 Gene and its Expression Analysis Under Abiotic Stresses in Rosa chinensis‘Yueyuehong’

MA Yuwan，LIU Ao，LIU Xiangdong，ZHANG Yajing，DONG Xuanke，LI Yufan^*(liyufan@hunau.edu.cn)，and CHEN Jiren^*(bjfucjr@126.com)

Hunan Mid-subtropical Quality Plant Breeding and Utilization Engineering Technology Research Center，College of Horticulture，Hunan Agricultural University，Changsha 410128，China

Received:2024-08-16 Revised:2024-12-09 Online:2025-04-25 Published:2025-04-25
Contact: LI Yufan，CHEN Jiren

Abstract

Abstract: In order to investigate the role of RcRAP2.7，a member of the AP2/ERF transcription factor family，in response to abiotic stress in Rosa chinensis‘Yueyuehong’. In this study，the full length of the coding sequence（CDS）of RcRAP2.7 was cloned，and then it was subjected to transcriptional expression analysis. The results showed that the CDS of RcRAP2.7 was 1 338 bp in length，encoding 446 amino acids，and there were two AP2/ERF conserved domains，located in 121–183 aa and 212–276 aa. And the RcRAP2.7 protein was located at the cell membrane and nucleus. Analysis of the promoter region regulatory elements indicated that RcRAP2.7 may be involved in hormone signalling pathways and stress response. Further transcriptional activation activity analysis showed that its C-terminal structural domain possessed transcriptional activation activity. Quantitative real-time PCR revealed that the expression pattern of RcRAP2.7 differed under various stress conditions such as drought，high salt and extreme temperature. In the leaves，the expression of RcRAP2.7 was down-regulated in high temperature and drought stress as a whole compared with the control，while it was first up-regulated，then down-regulated and then up-regulated in low temperature and high salt stress compared with the control. In the roots，its expression was affected by drought，high salt and extreme temperature，all of which showed a gradual increase with increasing treatment time，with the highest expression at 48 h of stress treatment.

Key words: Rosa chinensis, RcRAP2.7, abiotic stress, expression mode

CLC Number:

S 685.12

MA Yuwan, LIU Ao, LIU Xiangdong, ZHANG Yajing, DONG Xuanke, LI Yufan, CHEN Jiren. Cloning of the RcRAP2.7 Gene and its Expression Analysis Under Abiotic Stresses in Rosa chinensis‘Yueyuehong’[J]. Acta Horticulturae Sinica, 2025, 52(4): 921-932.

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Cloning of the RcRAP2.7 Gene and its Expression Analysis Under Abiotic Stresses in Rosa chinensis‘Yueyuehong’

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