Identification and Expression Analysis of Peach JMJ Histone Demethylase Gene Family

doi:10.16420/j.issn.0513-353x.2024-0105

Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (3): 575-590.doi: 10.16420/j.issn.0513-353x.2024-0105

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles

Identification and Expression Analysis of Peach JMJ Histone Demethylase Gene Family

WANG Yunyun¹^,², ZHOU Hui², QIU Keli¹, PAN Haifa², SHENG Yu², SHI Pei², XIE Qingmei², CHEN Hongli², ZHANG Jinyun²^,^**(), LI Dahui¹^,^**()

¹ College of Life Sciences，Anhui Agricultural University，Hefei 230036，China
² Key Laboratory of Germplasm Creation and Utilization of Horticultural Crops，Ministry of Agriculture and Rural Affairs，Institute of Horticulture，Anhui Academy of Agricultural Sciences，Hefei 230001，China

Received:2024-10-25 Revised:2025-01-14 Online:2025-03-25 Published:2025-03-25
Contact: ZHANG Jinyun, LI Dahui

Abstract

Abstract:

In order to understand the function of histone methylation in growth and response to abiotic stress in peach trees，genome-wide identification of histone demethylase（JMJ）family genes containing the JmjC domain was performed. Twenty-one JMJs were identified in the peach genome，which were divided into five subfamilies according to their phylogenetic relationships. There were significant differences among the members of these families，with exons ranging from 7 to 33，proteins containing 12 conserved domains，and the upstream regions of gene promoters containing different numbers of stress response，hormone response，and developmental regulatory elements. Using PpJMJ5 as bait，a potential interacting protein PpCIPK1 was found through yeast two-hybrid screening library. It was proved that the interaction between PpJMJ5 and PpCIPK1 existed，which may interfere with the interaction between calhomodin and its interacting protein CBL-CIPK to some extent. PpJMJ5 may play an important role in the abiotic stress response of peach trees.

Key words: peach, histone demethylation, abiotic stress, JMJ

WANG Yunyun, ZHOU Hui, QIU Keli, PAN Haifa, SHENG Yu, SHI Pei, XIE Qingmei, CHEN Hongli, ZHANG Jinyun, LI Dahui. Identification and Expression Analysis of Peach JMJ Histone Demethylase Gene Family[J]. Acta Horticulturae Sinica, 2025, 52(3): 575-590.

Figures/Tables 13

References 36

[1]	Cao X M, Duan W Y, Wei C Y, Chen K S, Grierson D, Zhang B. 2019. Genome-wide identification and functional analysis of carboxylesterase and methylesterase gene families in peach(Prunus persica L. Batsch). Frontiers in Plant Science, 10:1511.
[2]	Chen S F, Zhou Y Q, Chen Y R, Gu J. 2018. Fastp:an ultra-fast all-in-one FASTQ preprocessor. Bioinformatics, 34:884-890.
[3]	Chen X S, Hu Y F, Zhou D X. 2011. Epigenetic gene regulation by plant Jumonji group of histone demethylase. Biochimica Et Biophysica Acta-Gene Regulatory Mechanisms, 1809:421-426.
[4]	Cheng K, Xu Y C, Yang C, Ouellette L, Niu L J, Zhou X C, Chu L T, Zhuang F, Liu J, Wu H L, Charron J B, Luo M. 2020. Histone tales:lysine methylation,a protagonist in Arabidopsis development. Journal of Experimental Botany, 71:793-807. doi: 10.1093/jxb/erz435 pmid: 31560751
[5]	Chen Yuansen, Chen Yunzhu, An Zeyu, Kong Lingzhe, Wang Xin, Li Yuhua, Wang Yu. 2023. Bioinformatics analysis of JmjC domain-containing histone demethylase fmily in Solanum lycopersicum. Molecular Plant Breeding, https://kns.cnki.net/kcms2/detail/46.1068.S.20230522.1127.004.html. in Chinese)
	陈元森, 陈韵竹, 安泽宇, 孔令哲, 王鑫, 李玉花, 王宇. 2023. 番茄JmjC结构域组蛋白去甲基化酶家族生物信息学分析. 分子植物育种, https://kns.cnki.net/kcms2/detail/46.1068.S.20230522.1127.004.html.
[6]	Cheng Y Z, He G Q, Yang S D, Ma S H, Ma J P, Shang F H Z, Li X F, Jin H Y, Guo D L. 2022. Genome-wide identification and expression analysis of JmjC domain-containing genes in grape under MTA treatment. Functional & Integrative Genomics, 22:783-795.
[7]	Gu T T, Han Y H, Huang R R, McAvoy R J, Li Y. 2016. Identification and characterization of histone lysine methylation modifiers in Fragaria vesca. Scientific Reports, 6:23581.
[8]	Han Y P, Li X Y, Cheng L, Liu Y C, Wang H, Ke D X, Yuan H Y, Zhang L S, Wang L. 2016. Genome-wide analysis of soybean JmjC domain-containing proteins suggests evolutionary conservation following whole-genome duplication. Frontiers in Plant Science, 7:224848.
[9]	Hollender C A, Pascal T, Tabb A, Hadiarto T, Srinivasan C, Wang W P, Liu Z C, Scorza R, Dardick C. 2018. Loss of a highly conserved sterile alpha motif domain gene(WEEP)results in pendulous branch growth in peach trees. Proceedings of the National Academy of Sciences of the United States of America, 115:E4690-E4699.
[10]	Hu H M, Du J M. 2022. Structure and mechanism of histone methylation dynamics in Arabidopsis. Current Opinion in Plant Biology, 67:102211.
[11]	Huang S Z, Zhang A, Jin J B, Zhao B, Wang T J, Wu Y F, Wang S, Liu Y T, Wang J, Guo P, Ahmad R, Liu B, Xu Z Y. 2019. Arabidopsis histone H3K4 demethylase JMJ17 functions in dehydration stress response. New Phytologist, 223:1372-1387.
[12]	Kamikawa Y F, Donohoe M E. 2014. The localization of histone H3K27me3 demethylase Jmjd3 is dynamically regulated. Epigenetics, 9:834-841. doi: 10.4161/epi.28524 pmid: 24646476
[13]	Kim D, Landmead B, Salzberg S L. 2015. HISAT:a fast spliced aligner with low memory requirements. Nature Methods,DOI:10.1038/nmeth.3317.
[14]	Klose R J, Kallin E M, Zhang Y. 2006. JmjC-domain-containing proteins and histone demethylation. Nature Reviews Genetics, 7:715-727.
[15]	Klose R J, Yi Z. 2007. Regulation of histone methylation by demethylimination and demethylation. Nature Reviews Molecular Cell Biology, 8:307-318. doi: 10.1038/nrm2143 pmid: 17342184
[16]	Kouzarides T. 2007. Chromatin modifications and their function. Cell, 128:693-705. doi: 10.1016/j.cell.2007.02.005 pmid: 17320507
[17]	Li Y, Cao K, Li N, Zhu G, Fang W, Chen C, Yu X, Guo J, Wang Q, Ding T, Wang J, Guan L, Wang J, Liu K, Guo W, Arús P, Huang S, Fei Z, Wang L. 2021. Genomic analyses provide insights into peach local adaptation and responses to climate change. Genome Research, 31:592-606. doi: 10.1101/gr.261032.120 pmid: 33687945
[18]	Li Z W, Jiang G X, Liu X C, Ding X C, Zhang D D, Wang X W, Zhou Y J, Yan H L, Li T T, Wu K Q, Jiang Y M, Duan X W. 2020. Histone demethylase SlJMJ6 promotes fruit ripening by removing H3K27 methylation of ripening-related genes in tomato. New Phytologist, 227:1138-1156. doi: 10.1111/nph.16590 pmid: 32255501
[19]	Liao Y, Smyth G K, Shi W. 2019. The R package Rsubread is easier,faster,cheaper and better for alignment and quantification of RNA sequencing reads. Nucleic Acids Research, 47:e47.
[20]	Liu C Y, Lu F L, Cui X, Cao X F. 2010. Histone methylation in higher plants. Annual Review of Plant Biology, 61:395-420. doi: 10.1146/annurev.arplant.043008.091939 pmid: 20192747
[21]	Liu J H, Ban Y, Wen X P, Nakajima I, Moriguchi T. 2009. Molecular cloning and expression analysis of an arginine decarboxylase gene from peach (Prunus persica). Gene, 429:10-17. doi: 10.1016/j.gene.2008.10.003 pmid: 18996450
[22]	Lu F L, Li G L, Cui X, Liu C Y, Wang X J, Cao X F. 2008. Comparative analysis of JmjC domain-containing proteins reveals the potential histone demethylases in Arabidopsis and rice. Journal of Integrative Plant Biology, 50:886-896.
[23]	Luo M, Hung F Y, Yang S G, Liu X C, Wu K Q. 2014. Histone lysine demethylases and their functions in plants. Plant Molecular Biology Reporter, 32:558-565.
[24]	Mirouze M, Paszkowski J. 2011. Epigenetic contribution to stress adaptation in plants. Current Opinion in Plant Biology, 14:267-274. doi: 10.1016/j.pbi.2011.03.004 pmid: 21450514
[25]	Qian Y X, Chen C L, Jiang L Y, Zhang J, Ren Q Y. 2019. Genome-wide identification,classification and expression analysis of the JmjC domain-containing histone demethylase gene family in maize. BMC Genomics, 20:1-16.
[26]	Qiu K L, Pan H F, Sheng Y, Wang Y Y, Shi P, Xie Q M, Zhang J Y, Zhou H. 2022. The Peach(Prunus persica)CBL and CIPK family genes:protein interaction profiling and expression analysis in response to various abiotic stresses. Plants-Basel, 11:3001.
[27]	Shi L, Cui X, Shen Y. 2023. The roles of histone methylation in the regulation of abiotic stress responses in plants. Plant Stress, http://10.1016/J.STRESS.2023.100303.
[28]	Tang R J, Wang C, Li K L, Luan S. 2020. The CBL-CIPK calcium signaling network:unified paradigm from 20 years of discoveries. Trends in Plant Science, 25:604-617.
[29]	Verde I, Jenkins J, Dondini L, Micali S, Pagliarani G, Vendramin E, Paris R, Aramini V, Gazza L, Rossini L, Bassi D, Troggio M, Shu S Q, Grimwood J, Tartarini S, Dettori M T, Schmutz J. 2017. The Peach v2.0 release:high-resolution linkage mapping and deep resequencing improve chromosome-scale assembly and contiguity. BMC Genomics,DOI:10.1186/s12864-017-3606-9.
[30]	Wang X H, Pan C L, Long J H, Bai S Y, Yao M M, Chen J J, Sun G, Fan Y L, Wang Z J, Liu F L, Liu C X, Li Q F. 2022. Genome-wide identification of the jumonji C domain- containing histone demethylase gene family in wheat and their expression analysis under drought stress. Frontiers in Plant Science, 13:987257.
[31]	Yan Y M, Tao H Y, He J H, Huang S Y. 2020. The HDOCK server for integrated protein-protein docking. Nature Protocols, 15:1829-1852. doi: 10.1038/s41596-020-0312-x pmid: 32269383
[32]	Zeng J, Jiang G X, Liang H Z, Yan H L, Kong X J, Duan X W, Li Z W. 2023. Histone demethylase MaJMJ 15 is involved in the regulation of postharvest banana fruit ripening. Food Chemistry, 407:135102.
[33]	Zhao Hua, Zhang Shuting, Liu Pudong, Fu Shuai, Ye Wei, Chen Yukun, Lin Yuling, Xu Han, Lai Zhongxiong. 2020. Genome-wide identification and expression analysis in response to different photoperiodic of JmjC gene family in Dendrobium. Acta Horticulturae Sinica, 47 (8):1505-1520. (in Chinese)
	赵桦, 张舒婷, 刘蒲东, 付帅, 叶炜, 陈裕坤, 林玉玲, 徐涵, 赖钟雄. 2020. 石斛JmjC基因家族的鉴定及响应不同光周期的表达分析. 园艺学报, 47 (8):1505-1520. doi: 10.16420/j.issn.0513-353x.2019-0933 URL
[34]	Zhou Huangjing, Wang Xiangwu, Ji Fengfeng, Zhang Penghui, Guo Qinghua, Hao Yunfei, He Shibin. 2020. Bioinformatics analysis of JmjC domain-containing histone demethylase family in watermelon[Citrullus lanatus(Thunb.)Matsum. & Nakai]. Plant Science Journal, 38:802-811. (in Chinese)
	周皇经, 王翔伍, 吉逢逢, 张鹏辉, 郭清华, 郝云飞, 何世斌. 2020. 西瓜含有JmjC结构域的组蛋白去甲基化酶家族的生物信息学分析. 植物科学学报, 38:802-811.
[35]	Zhou H, Sheng Y, Qiu K, Ren F, Shi P, Xie Q, Guo J, Pan H, Zhang J. 2023. Improved annotation of the peach(Prunus persica)genome and identification of tissue-or development stage-specific alternative splicing through the integration of Iso-Seq and RNA-Seq data. Horticulturae, 9:175.
[36]	Zhu Z F, Li Q Y, Gichuki D K, Hou Y J, Liu Y S, Zhou H M, Xu C, Fang L C, Gong L Z, Zheng B B, Duan W, Fan P G, Wang Q F, Xin H P. 2023. Genome-wide profiling of histone H3 lysine 27 trimethylation and its modification in response to chilling stress in grapevine leaves. Horticultural Plant Journal, 9 (3):496-508.

用途 Purpose	名称 Name	上游引物（5′-3′） Forward primer sequence	下游引物（3′-5′） Reverse primer sequence
qRT-PCR	qPpJMJ5	CTGAATTGCGAAATAGGCTGG	GCCCCTGGAAGGACAAGAA
酵母双杂 Yeast two-hybrid	pGBKT7-PpJMJ5	ATGGCCATGGAGGCCGAATTCATGGCTTTTAATTCAGTTCCACCT	CCGCTGCAGGTCGACGGATCCTCACCTTTGCCTGGATGTTGA
酵母双杂 Yeast two-hybrid	pGADT7-PpJMJ5	GCCATGGAGGCCAGTGAATTCATGGCTTTTAATTCAGTTCCACCT	ATTCATCTGCAGCTCGAGCTCTCACCTTTGCCTGGATGTTGA
	pGBKT7-PpCBL5	ATGGCCATGGAGGCCGAATTCATGGGTTGTTTTAGTTCTAAAGCAA	CCGCTGCAGGTCGACGGATCCACGTAGCAATCTCATCAACCT
	pGADT7-PpCBL5	GCCATGGAGGCCAGTGAATTCATGGGTTGTTTTAGTTCTAAAGCAA	ATTCATCTGCAGCTCGAGCTCTCACGTAGCAATCTCATCAACCT
	pGBKT7-PpCIPK1	ATGGCCATGGAGGCCGAATTCATGGACCAAGGGGTCCCA	CCGCTGCAGGTCGACGGATCCTCAAACGACGACGTTATGCC
	pGADT7-PpCIPK1	GCCATGGAGGCCAGTGAATTCATGGACCAAGGGGTCCCA	ATTCATCTGCAGCTCGAGCTCTCAAACGACGACGTTATGCC
	pGADT7-PpCIPK2	GCCATGGAGGCCAGTGAATTCATGGGACTTGCCAGCAACA	ATTCATCTGCAGCTCGAGCTCTCAAGACGATGAATAGCCACG
	pGADT7-PpCIPK3	GCCATGGAGGCCAGTGAATTCATGGTGATCGTAAGAAAAGGTGC	ATTCATCTGCAGCTCGAGCTCTCACACTTGGCTAGTCAACAACC
	pGADT7-PpCIPK4	GCCATGGAGGCCAGTGAATTCATGGAAGAAAGGACTGTCTTGTTT	ATTCATCTGCAGCTCGAGCTCTCATGAGTCTTGTTGATCACCCT
	pGADT7-PpCIPK5	GCCATGGAGGCCAGTGAATTCATGGTGGTGAGGAAAGTGGGTA	ATTCATCTGCAGCTCGAGCTCTCAACGCTTTTTACTTTTACTCTTGC
	pGADT7-PpCIPK6	GCCATGGAGGCCAGTGAATTCATGGAGAAGATGAGTGCGGC	ATTCATCTGCAGCTCGAGCTCTTATTTCCTTGATCCTTCAGTAGTTTC
	pGADT7-PpCIPK7	GCCATGGAGGCCAGTGAATTCATGGAAAATAAGGGTAAGGTGTTGA	ATTCATCTGCAGCTCGAGCTCTTACTGTTTCTCCTCACCTTGCC
	pGADT7-PpCIPK10	GCCATGGAGGCCAGTGAATTCATGGCAAACCTCACCACCA	ATTCATCTGCAGCTCGAGCTCCTATTCAGAATCTGAAGGTAGATATGAAG
	pGADT7-PpCIPK11	GCCATGGAGGCCAGTGAATTCATGTCGTCGAGGTCGGCG	ATTCATCTGCAGCTCGAGCTCTCAAGATGGCAGAGCACCG
	pGADT7-PpCIPK12	GCCATGGAGGCCAGTGAATTCATGCCAGAGATCGAACAGCAG	ATTCATCTGCAGCTCGAGCTCTCAATCAGCTGGGCAGTGC
	pGADT7-PpCIPK13	GCCATGGAGGCCAGTGAATTCATGGAGAATAAATCGAATGTGCTG	ATTCATCTGCAGCTCGAGCTCCTATTGTGGCTGATGCTGTTCTAGT
	pGADT7-PpCIPK14	GCCATGGAGGCCAGTGAATTCATGAATCAACCAAAAATCA AGCG	ATTCATCTGCAGCTCGAGCTCTTACTTCACTTTTTGCATATCCTCCT
	pGADT7-PpCIPK15	GCCATGGAGGCCAGTGAATTCATGGAAAACAAAGGGAGTG TGTT	ATTCATCTGCAGCTCGAGCTCTTATGAATCCTGGGGCGAGA
	pGADT7-PpCIPK16	GCCATGGAGGCCAGTGAATTCATGCCGGAGATCGAGGTAGT	ATTCATCTGCAGCTCGAGCTCTTAATTACCGGCGACGTCC
	pGADT7-PpCIPK17	GCCATGGAGGCCAGTGAATTCATGAAGAAGGTGTCGAGAA AGGTAG	ATTCATCTGCAGCTCGAGCTCTCAGCAAGTCATTGTTCGAAGC
	pGADT7-PpCIPK18	GCCATGGAGGCCAGTGAATTCATGGCGGATGAAAAAGGCA	ATTCATCTGCAGCTCGAGCTCTCAAGCAGCCGTCGAACTG
	pGBKT7-PpCIPK1-1	ATGGCCATGGAGGCCGAATTCATGGACCAAGGGGTCCCAC	CCGCTGCAGGTCGACGGATCCTCATACGGCGTCGCATTTCGA
	pGADT7-PpCIPK1-1	GCCATGGAGGCCAGTGAATTCATGGACCAAGGGGTCCCAC	ATTCATCTGCAGCTCGAGCTCTCATACGGCGTCGCATTTCGA
	pGBKT7-PpCIPK1-2	ATGGCCATGGAGGCCGAATTCGAGGAGGAGAAGTCGAAAT	CCGCTGCAGGTCGACGGATCCTCAAACGACGACGTTATGCCA
	pGADT7-PpCIPK1-2	GCCATGGAGGCCAGTGAATTCGAGGAGGAGAAGTCGAAATGCG	ATTCATCTGCAGCTCGAGCTCTCAAACGACGACGTTATGCCA
LCI	PpJMJ5-Cluc	TACGCGTCCCGGGGCGGTACCATGGCTTTTAATTCAGTTCCACCT	ACGAAAGCTCTGCAGGTCGACTCACCTTTGCCTGGATGTTGA
LCI	PpCIPK1-Nluc	ACGGGGGACGAGCTCGGTACCATGGACCAAGGGGTCCCAC	CGCGTACGAGATCTGGTCGACAACGACGACGTTATGCCACG

用途 Purpose	名称 Name	上游引物（5′-3′） Forward primer sequence	下游引物（3′-5′） Reverse primer sequence
qRT-PCR	qPpJMJ5	CTGAATTGCGAAATAGGCTGG	GCCCCTGGAAGGACAAGAA
酵母双杂 Yeast two-hybrid	pGBKT7-PpJMJ5	ATGGCCATGGAGGCCGAATTCATGGCTTTTAATTCAGTTCCACCT	CCGCTGCAGGTCGACGGATCCTCACCTTTGCCTGGATGTTGA
酵母双杂 Yeast two-hybrid	pGADT7-PpJMJ5	GCCATGGAGGCCAGTGAATTCATGGCTTTTAATTCAGTTCCACCT	ATTCATCTGCAGCTCGAGCTCTCACCTTTGCCTGGATGTTGA
	pGBKT7-PpCBL5	ATGGCCATGGAGGCCGAATTCATGGGTTGTTTTAGTTCTAAAGCAA	CCGCTGCAGGTCGACGGATCCACGTAGCAATCTCATCAACCT
	pGADT7-PpCBL5	GCCATGGAGGCCAGTGAATTCATGGGTTGTTTTAGTTCTAAAGCAA	ATTCATCTGCAGCTCGAGCTCTCACGTAGCAATCTCATCAACCT
	pGBKT7-PpCIPK1	ATGGCCATGGAGGCCGAATTCATGGACCAAGGGGTCCCA	CCGCTGCAGGTCGACGGATCCTCAAACGACGACGTTATGCC
	pGADT7-PpCIPK1	GCCATGGAGGCCAGTGAATTCATGGACCAAGGGGTCCCA	ATTCATCTGCAGCTCGAGCTCTCAAACGACGACGTTATGCC
	pGADT7-PpCIPK2	GCCATGGAGGCCAGTGAATTCATGGGACTTGCCAGCAACA	ATTCATCTGCAGCTCGAGCTCTCAAGACGATGAATAGCCACG
	pGADT7-PpCIPK3	GCCATGGAGGCCAGTGAATTCATGGTGATCGTAAGAAAAGGTGC	ATTCATCTGCAGCTCGAGCTCTCACACTTGGCTAGTCAACAACC
	pGADT7-PpCIPK4	GCCATGGAGGCCAGTGAATTCATGGAAGAAAGGACTGTCTTGTTT	ATTCATCTGCAGCTCGAGCTCTCATGAGTCTTGTTGATCACCCT
	pGADT7-PpCIPK5	GCCATGGAGGCCAGTGAATTCATGGTGGTGAGGAAAGTGGGTA	ATTCATCTGCAGCTCGAGCTCTCAACGCTTTTTACTTTTACTCTTGC
	pGADT7-PpCIPK6	GCCATGGAGGCCAGTGAATTCATGGAGAAGATGAGTGCGGC	ATTCATCTGCAGCTCGAGCTCTTATTTCCTTGATCCTTCAGTAGTTTC
	pGADT7-PpCIPK7	GCCATGGAGGCCAGTGAATTCATGGAAAATAAGGGTAAGGTGTTGA	ATTCATCTGCAGCTCGAGCTCTTACTGTTTCTCCTCACCTTGCC
	pGADT7-PpCIPK10	GCCATGGAGGCCAGTGAATTCATGGCAAACCTCACCACCA	ATTCATCTGCAGCTCGAGCTCCTATTCAGAATCTGAAGGTAGATATGAAG
	pGADT7-PpCIPK11	GCCATGGAGGCCAGTGAATTCATGTCGTCGAGGTCGGCG	ATTCATCTGCAGCTCGAGCTCTCAAGATGGCAGAGCACCG
	pGADT7-PpCIPK12	GCCATGGAGGCCAGTGAATTCATGCCAGAGATCGAACAGCAG	ATTCATCTGCAGCTCGAGCTCTCAATCAGCTGGGCAGTGC
	pGADT7-PpCIPK13	GCCATGGAGGCCAGTGAATTCATGGAGAATAAATCGAATGTGCTG	ATTCATCTGCAGCTCGAGCTCCTATTGTGGCTGATGCTGTTCTAGT
	pGADT7-PpCIPK14	GCCATGGAGGCCAGTGAATTCATGAATCAACCAAAAATCA AGCG	ATTCATCTGCAGCTCGAGCTCTTACTTCACTTTTTGCATATCCTCCT
	pGADT7-PpCIPK15	GCCATGGAGGCCAGTGAATTCATGGAAAACAAAGGGAGTG TGTT	ATTCATCTGCAGCTCGAGCTCTTATGAATCCTGGGGCGAGA
	pGADT7-PpCIPK16	GCCATGGAGGCCAGTGAATTCATGCCGGAGATCGAGGTAGT	ATTCATCTGCAGCTCGAGCTCTTAATTACCGGCGACGTCC
	pGADT7-PpCIPK17	GCCATGGAGGCCAGTGAATTCATGAAGAAGGTGTCGAGAA AGGTAG	ATTCATCTGCAGCTCGAGCTCTCAGCAAGTCATTGTTCGAAGC
	pGADT7-PpCIPK18	GCCATGGAGGCCAGTGAATTCATGGCGGATGAAAAAGGCA	ATTCATCTGCAGCTCGAGCTCTCAAGCAGCCGTCGAACTG
	pGBKT7-PpCIPK1-1	ATGGCCATGGAGGCCGAATTCATGGACCAAGGGGTCCCAC	CCGCTGCAGGTCGACGGATCCTCATACGGCGTCGCATTTCGA
	pGADT7-PpCIPK1-1	GCCATGGAGGCCAGTGAATTCATGGACCAAGGGGTCCCAC	ATTCATCTGCAGCTCGAGCTCTCATACGGCGTCGCATTTCGA
	pGBKT7-PpCIPK1-2	ATGGCCATGGAGGCCGAATTCGAGGAGGAGAAGTCGAAAT	CCGCTGCAGGTCGACGGATCCTCAAACGACGACGTTATGCCA
	pGADT7-PpCIPK1-2	GCCATGGAGGCCAGTGAATTCGAGGAGGAGAAGTCGAAATGCG	ATTCATCTGCAGCTCGAGCTCTCAAACGACGACGTTATGCCA
LCI	PpJMJ5-Cluc	TACGCGTCCCGGGGCGGTACCATGGCTTTTAATTCAGTTCCACCT	ACGAAAGCTCTGCAGGTCGACTCACCTTTGCCTGGATGTTGA
LCI	PpCIPK1-Nluc	ACGGGGGACGAGCTCGGTACCATGGACCAAGGGGTCCCAC	CGCGTACGAGATCTGGTCGACAACGACGACGTTATGCCACG

亚家族 Subfamily	基因 Gene	ID	染色体位置 Chromosomal location	蛋白质长度/aa Protein length	等电点pI	亚细胞定位 Subcellular localization
KDM4/HDM3	PpJMJ1	Prupe.2G211400	2	1 553	6.08	细胞核Cell nucleus
	PpJMJ2	Prupe.5G159200	5	1 486	8.73	细胞核Cell nucleus
	PpJMJ3	Prupe.5G028800	5	893	8.27	细胞核Cell nucleus
KDM5/JARID	PpJMJ4	Prupe.6G349900	6	1 227	6.26	细胞核Cell nucleus
	PpJMJ5	Prupe.8G222100	8	709	7.93	细胞核Cell nucleus
	PpJMJ6	Prupe.4G102200	4	1 067	5.64	细胞核Cell nucleus
	PpJMJ7	Prupe.8G028600	8	461	6.15	细胞核Cell nucleus
	PpJMJ8	Prupe.5G099000	5	1 851	6.66	细胞核Cell nucleus
JMJD6	PpJMJ9	Prupe.1G521300	1	1 001	5.33	细胞质Cytoplasm
	PpJMJ10	Prupe.7G089800	7	521	5.91	线粒体Mitochondria
	PpJMJ11	Prupe.6G322900	6	942	5.78	细胞核Cell nucleus
KDM3/JHDM2	PpJMJ12	Prupe.2G123400	2	853	8.70	细胞核Cell nucleus
	PpJMJ13	Prupe.5G015100	5	979	6.72	细胞核Cell nucleus
	PpJMJ14	Prupe.4G016300	4	867	5.60	细胞核Cell nucleus
	PpJMJ15	Prupe.2G309900	2	1 770	8.12	细胞核Cell nucleus
	PpJMJ16	Prupe.2G189600	2	1 051	6.86	细胞核Cell nucleus
	PpJMJ17	Prupe.4G034800	4	1 031	8.56	细胞核Cell nucleus
	PpJMJ18	Prupe.5G169900	5	480	5.09	细胞质Cytoplasm
JMJC domain-only	PpJMJ19	Prupe.6G015700	6	407	5.37	细胞核Cell nucleus
	PpJMJ20	Prupe.8G234700	8	556	5.11	细胞核Cell nucleus
	PpJMJ21	Prupe.4G147600	4	342	5.18	细胞核Cell nucleus

亚家族 Subfamily	基因 Gene	ID	染色体位置 Chromosomal location	蛋白质长度/aa Protein length	等电点pI	亚细胞定位 Subcellular localization
KDM4/HDM3	PpJMJ1	Prupe.2G211400	2	1 553	6.08	细胞核Cell nucleus
	PpJMJ2	Prupe.5G159200	5	1 486	8.73	细胞核Cell nucleus
	PpJMJ3	Prupe.5G028800	5	893	8.27	细胞核Cell nucleus
KDM5/JARID	PpJMJ4	Prupe.6G349900	6	1 227	6.26	细胞核Cell nucleus
	PpJMJ5	Prupe.8G222100	8	709	7.93	细胞核Cell nucleus
	PpJMJ6	Prupe.4G102200	4	1 067	5.64	细胞核Cell nucleus
	PpJMJ7	Prupe.8G028600	8	461	6.15	细胞核Cell nucleus
	PpJMJ8	Prupe.5G099000	5	1 851	6.66	细胞核Cell nucleus
JMJD6	PpJMJ9	Prupe.1G521300	1	1 001	5.33	细胞质Cytoplasm
	PpJMJ10	Prupe.7G089800	7	521	5.91	线粒体Mitochondria
	PpJMJ11	Prupe.6G322900	6	942	5.78	细胞核Cell nucleus
KDM3/JHDM2	PpJMJ12	Prupe.2G123400	2	853	8.70	细胞核Cell nucleus
	PpJMJ13	Prupe.5G015100	5	979	6.72	细胞核Cell nucleus
	PpJMJ14	Prupe.4G016300	4	867	5.60	细胞核Cell nucleus
	PpJMJ15	Prupe.2G309900	2	1 770	8.12	细胞核Cell nucleus
	PpJMJ16	Prupe.2G189600	2	1 051	6.86	细胞核Cell nucleus
	PpJMJ17	Prupe.4G034800	4	1 031	8.56	细胞核Cell nucleus
	PpJMJ18	Prupe.5G169900	5	480	5.09	细胞质Cytoplasm
JMJC domain-only	PpJMJ19	Prupe.6G015700	6	407	5.37	细胞核Cell nucleus
	PpJMJ20	Prupe.8G234700	8	556	5.11	细胞核Cell nucleus
	PpJMJ21	Prupe.4G147600	4	342	5.18	细胞核Cell nucleus

Identification and Expression Analysis of Peach JMJ Histone Demethylase Gene Family

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 13

References 36

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	DENG Shuqin, GAO Yingrui, LI Yutong, WANG Ying, GONG Chunmei, BAI Juan. Response of Ubiquitin-ligase Gene CsPUB21 to Different Abiotic Stress in Camellia sinensis [J]. Acta Horticulturae Sinica, 2025, 52(3): 655-670.
[2]	LIU Jianhao, JING Yanfu, LIU Yuexin, XU Yaoguang, YU Yang, GE Xiuxiu, XIE Hua. Identification of Peach NAC Gene Family and Role of PpNAC050 in Promoting Fruit Fructose Accumulation [J]. Acta Horticulturae Sinica, 2024, 51(9): 1983-1996.
[3]	ZHANG Fan, YANG Yang, WANG Hong, ZHANG Xuebing. Improving Physicochemical Properties and Bacterial Communities of Peach-Heavy Soils Using Biofertilizers and Organic Fertilizers [J]. Acta Horticulturae Sinica, 2024, 51(9): 2089-2104.
[4]	LI Yadi, WANG Hanxiang, HU Baigeng, YANG Hui, HU Xinxi, XIONG Xingyao, WANG Wanxing. Research Progress on Plant Growth Promoting Rhizobacteria to Alleviate Abiotic Stress Tolerance of Horticultural Crops [J]. Acta Horticulturae Sinica, 2024, 51(8): 1964-1976.
[5]	LI Yachen, ZHENG Yanmei, SONG Wenpei, LI Dawei, LIANG Hong, ZHANG Xianzhi. Research Progress on the Role of Hydrogen-Rich Water in Plant Growth and Development and Stress Response [J]. Acta Horticulturae Sinica, 2024, 51(7): 1489-1500.
[6]	LUO Xinrui, ZHANG Xiaoxu, WANG Yuping, WANG Zhi, MA Yuanyuan, ZHOU Bingyue. Identification and Expression Analysis of Trihelix Gene Family in Common Bean [J]. Acta Horticulturae Sinica, 2024, 51(12): 2775-2790.
[7]	ZHANG Zexiong, QIU Yuanxin, MO Guanlian, CHEN Caiyun, LIU Jianliang, WANG Qin, ZHONG Le, XIE Hongfeng. Preparation of Fermented Fertilizer from the Waste of Fish，Peanuts，and Soybean and Its Effect on Peach Quality [J]. Acta Horticulturae Sinica, 2024, 51(10): 2386-2400.
[8]	CHEN Xuesen, WANG Nan, PENG Futian, MAO Zhiquan, YIN Chengmiao, JIANG Yuanmao, GE Shunfeng, HU Dagang, LI Yuanyuan, DU Yuanpeng, YAO Yuxin, ZHANG Zongying. Advances in Quality and Maturity Breeding of Important Deciduous Fruit Trees in China [J]. Acta Horticulturae Sinica, 2024, 51(1): 8-26.
[9]	SHI Meng, LIU Fengzhi, WANG Haibo, WANG Yingying, SHI Xiangbin, WANG Zhiqiang, LI Peng, LIU Wanchun, and WANG Xiaodi. A New Mid-ripening and Cold Resistant Peach Cultivar‘Zhonghan Cuixia’ [J]. Acta Horticulturae Sinica, 2023, 50(S1): 15-16.
[10]	YANG Xingwang, WANG Yingying, WANG Haibo, WANG Xiaolong, WANG Zhiqiang, SHI Xiangbin, JI Xiaohao, LIU Wanchun, and WANG Xiaodi. A New Mid-ripening and Cold Resistant Peach Cultivar‘Zhonghan Hongqiong’ [J]. Acta Horticulturae Sinica, 2023, 50(S1): 17-18.
[11]	DUAN Wenyi, NIU Liang, CUI Guochao, ZENG Wenfang, PAN Lei, SUN Shihang, and WANG Zhiqiang. A Late-ripening Yellow Peach Cultivar‘Zhongtaohuangjinmi No. 5’ [J]. Acta Horticulturae Sinica, 2023, 50(S1): 23-24.
[12]	ZHANG Pan, YU Yongxu, CAO Linggai, ZHU Guangbing, WU Wei, GUO Yushuang, YIN Guoying, JIA Meng’ao. Research Progress of m⁶A Methylation Modification Response to Plant Biotic and Abiotic Stresses [J]. Acta Horticulturae Sinica, 2023, 50(9): 1841-1853.
[13]	WEI Jianming, LI Yunzhou, LIANG Yan. Advances in Research on Improving Tomato Disease Resistance and Stress Resistance by Grafting Technology [J]. Acta Horticulturae Sinica, 2023, 50(9): 1997-2014.
[14]	ZHOU Cheng, FANG Yi, ZHOU Jinyang, HUANG Qihao, PAN Yongjian, SHI Qianqian, NI Huixian, YANG Zhenfeng, SONG Chunbo. The Relationship Between Membrane Lipid Metabolism and Chilling Injury of Postharvest Peach Fruit Induced by Low Temperature [J]. Acta Horticulturae Sinica, 2023, 50(6): 1305-1317.
[15]	XIAO Xiang, ZHOU Chujiang, JIN Shuwan, SHI Liyu, YANG Zhenfeng, CAO Shifeng, CHEN Wei. Mechanism of PpMADS2 and PpMADS3 Synergistically Regulating Carotenoids Accumulation in Peach Fruit [J]. Acta Horticulturae Sinica, 2023, 50(6): 1173-1186.