CsPUB54 Negatively Regulates Cucumber Resistance to Phytophthora Melonis by Interacting with PmRXLR1 Effector

doi:10.16420/j.issn.0513-353x.2024-0274

Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (3): 591-602.doi: 10.16420/j.issn.0513-353x.2024-0274

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles

CsPUB54 Negatively Regulates Cucumber Resistance to Phytophthora Melonis by Interacting with PmRXLR1 Effector

WANG Rui¹^,², WU Hongfei¹^,³, ZHANG Changyuan¹, CAO Haishun¹, TAN Delong¹, GUO Jinju¹, WANG Yunlong¹, WANG Rufang¹, YUAN Yu¹, WU Tingquan¹^,²^,^*()

¹ Institute of Facility Agriculture，Guangdong Academy of Agricultural Sciences，Guangzhou 510640，China
² Vegetable Research Institute，Guangdong Academy of Agricultural Sciences，Guangzhou 510640，China
³ Zhongkai University of Agriculture and Engineering，Guangzhou 510225，China

Received:2024-12-30 Revised:2025-02-13 Online:2025-03-25 Published:2025-03-25
Contact: WU Tingquan

Abstract

Abstract:

B80 cucumber with highly susceptible to Phytophthora blight，and Phytophthora melonis（P. melonis）were used as experimental materials. The gene that encodes a PUB protein containing the U-box domain from B80，named CsPUB54，and a RXLR effector protein gene，named PmRXLR1 from P. mellonis were cloned. Experimental results of transcriptome and qRT-PCR of cucumber leaves inoculated with P. mellonis for 24 hours both showed that the infection could induce rapid increase of gene expression levels of CsPUB54 and PmRXLR1. CsPUB54 gene silencing through transient gene silencing method could increase the resistance of cucumber cotyledons to P. melonis. In addition，the results of yeast two hybrid and Pull-down experiments confirmed that CsPUB54 could interact with PmRXLR1 both in vivo and in vitro. Using the method of base mutation，the W and Y functional domains of PmRXLR1 were mutated respectively，resulting in 7 mutants. The interaction detection between these mutants and CsPUB54 showed that the W and Y functional domains were necessary for the interaction between PmRXLR1 and CsPUB54，and the isoleucine（I）in the W functional domain was a key amino acid required for the interaction. Genetic evolution analysis of CsPUB54 and PUB proteins related to plant immune negative regulatory that have been published showed that CsPUB54 had a low genetic variation rate and the closest relationship with AtPUB22 and AtPUB23 in Arabidopsis thaliana. These research data suggest that CsPUB54 can negatively regulate cucumber resistance to P. melonis through interaction with PmRXLR1.

Key words: cucumber, Phytophthora blight, Phytophthora meloni, protein interaction, negative regulation, resistance

WANG Rui, WU Hongfei, ZHANG Changyuan, CAO Haishun, TAN Delong, GUO Jinju, WANG Yunlong, WANG Rufang, YUAN Yu, WU Tingquan. CsPUB54 Negatively Regulates Cucumber Resistance to Phytophthora Melonis by Interacting with PmRXLR1 Effector[J]. Acta Horticulturae Sinica, 2025, 52(3): 591-602.

Figures/Tables 9

References 32

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名称Name	编码序列Coding sequence	蛋白序列Protein sequence
CsPUB54	ATGGATGAACAAATCGAAGTCCCCCACTATTTCCTCTGCCCCATCTCCCTCCAAATCATGAAAGACCCTGTAACTCTTCCCTCCGGCATCACCTACGATCGCCACTCCATTGAAACTTGG CTCTTCTCCGGCAAAAACTCCTCCTGCCCCGTCACTAAACTCCCAGTCTCCGACTCCGATTCCGATCTCCTCACCCCCAACCACACCCTCCGCCGTCTCATCCAAGCTTGGTGTACTTTGA ACTCCTCCCATGGCGTCGAACGTTTCCCCACTCCTAAACCCCCCATTCATAAATCCCAAATCCTTCACATTATTTCCACTTCCAACACTTCCCCTTCTTCCCAAATCTCCTCCATTCGCCG TCTCCGCTCCATTTCCGCCGAGTCTGAAACTAATCGCCGATGCGTTGAATTCGCCGGTGCGCCGGAGTTTTTAGTCTCTGTTATTGTGGGTTCTGATTCCTCGGCTTCTCATGAAGCCCTCT CCACTCTTCATAACCTCCGTCTCTCTGATTCAACGTTTAAATCATTGGCTACCCGCCCTGAATTCCTCGAGTCCTTGACCGATTTCATGAAATTACAACAGGGTACTCATGAATCGTCCAGA ACGTACGCGGTGTTGATTTTGAAATCAATTATTGAAGTCGCTGAACCAATTCAACTAAGCTTCTTGAAACCCGAATTGTTTGTGCAAATCGTTGAGATTTTGAAAGATCGATCATCCTCTCAA CAGATTTTCAAAGCGGCATTGGGTATTTTGATTGCGGTGAGTCCATTGGGGAGAAACA GACTGAAGGCAGTAGAAGCAGGTGGAGTTAGGGCTTTGGTTGAGATTTTACTGTCGTCGCCGGAAAAAAGAGTATGTGAAATGACATTGACGGCGATGGATATACTGTGTGGGTGTGCGGAT GGAAGAGCGGCGCTGTTGGCACACGGCGGAGGGATGGCAGTGGTTTCGAAGAAGATATTGAGAGTGTCGCAATTGGGGAGTGAAAGGGCGGTGAGAATATTGTATTCGGTGGCTAAATTCTCA GGAAGTCCTGCGGTGTTGATGGAAATGGCGCAACTGGGGATTGTGGCAAAGCTATGTTTGGTTCTGCAAATTGAAAATGGAGGCAAGACGAAGGAGAAAGCTAAAGAGATTTTGAAAATGCAT TCTCGTCTTTGGAAGAACTCACCTTGTATTCCTTCTAAATTGGCTTCTTCATATCCTACAAATTAA	MDEQIEVPHYFLCPISLQIMKDPVTLPSGITYDRHSIETWLFSGKNSSCPVTKLPVSDSDSDLLTPNHTLRRLIQAWCTLNSSHGVERFPTPKPPIHKSQILHIISTSNTSPSSQISSIRRLRS ISAESETNRRCVEFAGAPEFLVSVIVGSDSSASHEALSTLHNLRLSDSTFKSLATRPEFLESLTDFMKLQQGTHESSRTYAVLILKSIIEVAEPIQLSFLKPELFVQIVEILKDRSSSQQIFKAA LGILIAVSPLGRNRLKAVEAGGVRALVEILLSSPEKRVCEMTLTAMDILCGCADGRAALLAHGGGMAVVSKKILRVSQLGSERAVRILYSVAKFSGSPAVLMEMAQLGIVAKLCLVLQIENGGKTK EKAKEILKMHSRLWKNSPCIPSKLASSYPTN^*
PmRXLR1	ATGCGTCTGTCTTTCATGCTTTCTGTTGCTGTGGTCATCGGCCACCTCGTGGCCTGCAACGCGACTGC GGACTTCGACGGAACTGAAGTGTCCACGATGGAGTCGCCAGACATCGTCCGGCCCTCG CTCAAGAACGACGACATTGCCGGTGGGAGGTTGCTGCGATCCCACCAAGAGGACGACGCGGAGGAACGGACCTTCATCATTACTGAACTGATGAACAAGGCGGCGGTTAAAAAGTTGGCCAGGCAG ATGCTGAAGGACCCATCAAAGGAGAAGGAAGCGTACGAGGCGTGGGCAGCGAACAAGTACACCCTGGTCCAAATCAAGAACTGGCTGAAAATCGGGGACCCCAAGAACAAAGGAAAATATGACCG CATCTACAACGGGTACGGCTTTCACCTGTATCAGGATTAA	MRLSFMLSVAVVIGHLVACNATADFDGTEVSTMESPDIVRPSLKNDDIAGGRLLRSHQEDDAEERTFIITELMNKAAVKKLARQMLKDPSKEKE AYEAWAANKYTLVQIKNWLKIGDPKNKGKYDRIYNGYGFHLYQD^*
PmRXLR1 （无信号肽 No signal peptide）	ACTGCGGACTTCGACGGAACTGAAGTGTCCACGATGGAGTCGCCAGACATCGTCCGGCCCTCGCTCAAGAACGACGACATTGCCGGTGGGAGGTTGCTGCGATCCCACCAAGAGGACGACGCGG AGGAACGGACCTTCATCATTACTGAACTGATGAACAAGGCGGCGGTTAAAAAGTTGGCCAGGCAGATGCTGAAGGACCCATCAAAGGAGAAGGAAGCGTACGAGGCGTGGGCAGCGAACAAGTA CACCCTGGTCCAAATCAAGAACTGGCTGAAAATCGGGGACCCCAAGAACAAAGGAAAATATGACCGCATC TACAACGGGTACGGCTTTCACCTGTATCAGGATTAA	TADFDGTEVSTMESPDIVRPSLKNDDIAGGRLLRSHQEDDAEERTFIITELMNKAAVKKLARQMLKDPSKEKEAYEA WAANKYTLVQIKNWLKIGDPKNKGKYDRIYNGYGFHLYQD^*

名称Name	编码序列Coding sequence	蛋白序列Protein sequence
CsPUB54	ATGGATGAACAAATCGAAGTCCCCCACTATTTCCTCTGCCCCATCTCCCTCCAAATCATGAAAGACCCTGTAACTCTTCCCTCCGGCATCACCTACGATCGCCACTCCATTGAAACTTGG CTCTTCTCCGGCAAAAACTCCTCCTGCCCCGTCACTAAACTCCCAGTCTCCGACTCCGATTCCGATCTCCTCACCCCCAACCACACCCTCCGCCGTCTCATCCAAGCTTGGTGTACTTTGA ACTCCTCCCATGGCGTCGAACGTTTCCCCACTCCTAAACCCCCCATTCATAAATCCCAAATCCTTCACATTATTTCCACTTCCAACACTTCCCCTTCTTCCCAAATCTCCTCCATTCGCCG TCTCCGCTCCATTTCCGCCGAGTCTGAAACTAATCGCCGATGCGTTGAATTCGCCGGTGCGCCGGAGTTTTTAGTCTCTGTTATTGTGGGTTCTGATTCCTCGGCTTCTCATGAAGCCCTCT CCACTCTTCATAACCTCCGTCTCTCTGATTCAACGTTTAAATCATTGGCTACCCGCCCTGAATTCCTCGAGTCCTTGACCGATTTCATGAAATTACAACAGGGTACTCATGAATCGTCCAGA ACGTACGCGGTGTTGATTTTGAAATCAATTATTGAAGTCGCTGAACCAATTCAACTAAGCTTCTTGAAACCCGAATTGTTTGTGCAAATCGTTGAGATTTTGAAAGATCGATCATCCTCTCAA CAGATTTTCAAAGCGGCATTGGGTATTTTGATTGCGGTGAGTCCATTGGGGAGAAACA GACTGAAGGCAGTAGAAGCAGGTGGAGTTAGGGCTTTGGTTGAGATTTTACTGTCGTCGCCGGAAAAAAGAGTATGTGAAATGACATTGACGGCGATGGATATACTGTGTGGGTGTGCGGAT GGAAGAGCGGCGCTGTTGGCACACGGCGGAGGGATGGCAGTGGTTTCGAAGAAGATATTGAGAGTGTCGCAATTGGGGAGTGAAAGGGCGGTGAGAATATTGTATTCGGTGGCTAAATTCTCA GGAAGTCCTGCGGTGTTGATGGAAATGGCGCAACTGGGGATTGTGGCAAAGCTATGTTTGGTTCTGCAAATTGAAAATGGAGGCAAGACGAAGGAGAAAGCTAAAGAGATTTTGAAAATGCAT TCTCGTCTTTGGAAGAACTCACCTTGTATTCCTTCTAAATTGGCTTCTTCATATCCTACAAATTAA	MDEQIEVPHYFLCPISLQIMKDPVTLPSGITYDRHSIETWLFSGKNSSCPVTKLPVSDSDSDLLTPNHTLRRLIQAWCTLNSSHGVERFPTPKPPIHKSQILHIISTSNTSPSSQISSIRRLRS ISAESETNRRCVEFAGAPEFLVSVIVGSDSSASHEALSTLHNLRLSDSTFKSLATRPEFLESLTDFMKLQQGTHESSRTYAVLILKSIIEVAEPIQLSFLKPELFVQIVEILKDRSSSQQIFKAA LGILIAVSPLGRNRLKAVEAGGVRALVEILLSSPEKRVCEMTLTAMDILCGCADGRAALLAHGGGMAVVSKKILRVSQLGSERAVRILYSVAKFSGSPAVLMEMAQLGIVAKLCLVLQIENGGKTK EKAKEILKMHSRLWKNSPCIPSKLASSYPTN^*
PmRXLR1	ATGCGTCTGTCTTTCATGCTTTCTGTTGCTGTGGTCATCGGCCACCTCGTGGCCTGCAACGCGACTGC GGACTTCGACGGAACTGAAGTGTCCACGATGGAGTCGCCAGACATCGTCCGGCCCTCG CTCAAGAACGACGACATTGCCGGTGGGAGGTTGCTGCGATCCCACCAAGAGGACGACGCGGAGGAACGGACCTTCATCATTACTGAACTGATGAACAAGGCGGCGGTTAAAAAGTTGGCCAGGCAG ATGCTGAAGGACCCATCAAAGGAGAAGGAAGCGTACGAGGCGTGGGCAGCGAACAAGTACACCCTGGTCCAAATCAAGAACTGGCTGAAAATCGGGGACCCCAAGAACAAAGGAAAATATGACCG CATCTACAACGGGTACGGCTTTCACCTGTATCAGGATTAA	MRLSFMLSVAVVIGHLVACNATADFDGTEVSTMESPDIVRPSLKNDDIAGGRLLRSHQEDDAEERTFIITELMNKAAVKKLARQMLKDPSKEKE AYEAWAANKYTLVQIKNWLKIGDPKNKGKYDRIYNGYGFHLYQD^*
PmRXLR1 （无信号肽 No signal peptide）	ACTGCGGACTTCGACGGAACTGAAGTGTCCACGATGGAGTCGCCAGACATCGTCCGGCCCTCGCTCAAGAACGACGACATTGCCGGTGGGAGGTTGCTGCGATCCCACCAAGAGGACGACGCGG AGGAACGGACCTTCATCATTACTGAACTGATGAACAAGGCGGCGGTTAAAAAGTTGGCCAGGCAGATGCTGAAGGACCCATCAAAGGAGAAGGAAGCGTACGAGGCGTGGGCAGCGAACAAGTA CACCCTGGTCCAAATCAAGAACTGGCTGAAAATCGGGGACCCCAAGAACAAAGGAAAATATGACCGCATC TACAACGGGTACGGCTTTCACCTGTATCAGGATTAA	TADFDGTEVSTMESPDIVRPSLKNDDIAGGRLLRSHQEDDAEERTFIITELMNKAAVKKLARQMLKDPSKEKEAYEA WAANKYTLVQIKNWLKIGDPKNKGKYDRIYNGYGFHLYQD^*

用途Usage	引物名称Primer name	引物序列（5′-3′）Primer sequence
基因克隆 Gene cloning	CsPUB54-F (C) CsPUB54-R (C) PmRXLR1-F (C) PmRXLR1-R (C)	ATGGATGAACAAATCGAAGTCCCCCAC TTAATTTGTAGGATATGAAGAAGCCA ATGCGTCTGTCTTTCATGCTTTC TTAATCCTGATACAGGTGAAAGCCG
载体构建 Vector construction	CsPUB54-F (V) CsPUB54-R (V) PmRXLR1-F (V) PmRXLR1-R (V)	CATATGATGGATGAACAAATCG CGCGGATCCTTAATTTGTAGGATATG CCGGAATTCACTGCGGACTTCGACG CGCGGATCCTTAATCCTGATACAGGTG
基因表达 Gene expression	CsPUB54-F (E) CsPUB54-R (E)	CAAATCTCCTCCATTCGCCGTC TCATGAAATCGGTCAAGGACTC
黄瓜内参基因 Cucumber reference gene	CsUBQ-F CsUBQ-R	GCGTAAGAAGAAGACCTACACCA CCTTTCCAGAGTCATCGACCT
瓜类疫霉菌ITS检测 ITS detection of Phytophthora melons	DC6-F ITS4-R	5′-GAGGGACTTTTGGGTAATCA-3′ 5′-TCCTCCGCTTATTGATATGC-3′

用途Usage	引物名称Primer name	引物序列（5′-3′）Primer sequence
基因克隆 Gene cloning	CsPUB54-F (C) CsPUB54-R (C) PmRXLR1-F (C) PmRXLR1-R (C)	ATGGATGAACAAATCGAAGTCCCCCAC TTAATTTGTAGGATATGAAGAAGCCA ATGCGTCTGTCTTTCATGCTTTC TTAATCCTGATACAGGTGAAAGCCG
载体构建 Vector construction	CsPUB54-F (V) CsPUB54-R (V) PmRXLR1-F (V) PmRXLR1-R (V)	CATATGATGGATGAACAAATCG CGCGGATCCTTAATTTGTAGGATATG CCGGAATTCACTGCGGACTTCGACG CGCGGATCCTTAATCCTGATACAGGTG
基因表达 Gene expression	CsPUB54-F (E) CsPUB54-R (E)	CAAATCTCCTCCATTCGCCGTC TCATGAAATCGGTCAAGGACTC
黄瓜内参基因 Cucumber reference gene	CsUBQ-F CsUBQ-R	GCGTAAGAAGAAGACCTACACCA CCTTTCCAGAGTCATCGACCT
瓜类疫霉菌ITS检测 ITS detection of Phytophthora melons	DC6-F ITS4-R	5′-GAGGGACTTTTGGGTAATCA-3′ 5′-TCCTCCGCTTATTGATATGC-3′

突变体Mutant	序列Sequence
PmRXLR1 Δ1 碱基Base	ACTGCGGACTTCGACGGAACTGAAGTGTCCACGATGGA GTCGCCAGACATCGTCCGGCCCTCGCTCAAGAACGACGACATTGCCGGTGGGAGGTTGCTGCGA TCCCACCAAGAGGACGACGCGGAGGAAC GGACCTTCATCATTACTGAACTGATGAACAAGGCGGCGGTTAA AAAGTTGGCCAGGCAGATGCTGAAGGACCCATCAAAGGAGAAGGAA---GGGGACCCCAAGAAC AAAGGAAAATATGACCGCATCTACAACGGGTACGGCTTTCACCTGTATCAGGATTAA
PmRXLR1 Δ1 氨基酸Amino acid	TADFDGTEVSTMESPDIVRPSLKNDDIAGGRLLRSH QEDDAEERTFIITELMNKAAVKKLARQMLKDPSKEKE---GDPKNKGKYDRIYNGYGFHLYQD*
PmRXLR1 Δ2 碱基Base	ACTGCGGACTTCGACGGAACTGAAGTGTCCACGATGGAGTCGCCAGACATCGTCCGG CCCTCGCTCAAGAACGACGACATTGCCGGTGGGAGGTTGCTGCGATCCCACCAAGAGGACGACGCGGAGGAACGGACCTTCATCATTACT GAACTGATGAACAAGGCGGCGGTTAAAAAGTTGGCCAGGCAGAT GCTGAAGGACCCATCAAAGGAGAAGGAAGCG GCGGAGGCG GCAGCAGCGAACAAGTACACC GGAGTCCAA GGAAAGAAC GCACTGAAAATCGGGGACCCCAAGAACAAAGGAAAATATGACCGCATCT ACAACGGGTACGGCTTTCACCTGTATCAGGATTAA
PmRXLR1 Δ2 氨基酸Amino acid	TADFDGTEVSTMESPDIVRPSLKNDDIAGGRLLRSH QEDDAEERTFIITELMNKAAVKKLARQMLKDPSKEKEAAEAAAANKYT GVQGKNALKIGDPKNKGKYDRIYNGYGFHLYQD*
PmRXLR1 Δ3 碱基Base	ACTGCGGACTTCGACGGAACTGAAGTGTCCACGATGGAGTCGCCAGACATCGTCCGGCCCTCG CTCAAGAACGACGACATTGCCGGTGGGAGGTTGCTGCGATCCCACCAAGAGGACGACGCGGAGG AACGGACCTTCATCATTACT GAACTGATGAACAAGGCGGCGGTTAAAAAGTTGGCCAGGCA GATGCTGAAGGACCCATCAAAGGAGAAGGAAGCGTAC TCCGCGTGGGCA TCCAACAAGTACACCCTG TCCTCCATCAAG TCCTGGCTGAAAATCGGGGACCCCAAGAACAAAGGAAA ATATGACCGCATCTACAACGGGTACG GCTTTCACCTGTATCAGGATTAA
PmRXLR1 Δ3 氨基酸Amino acid	TADFDGTEVSTMESPDIVRPSLKND DIAGGRLLRSHQEDDAEERTFIITELMNKAAVKKLA RQMLKDPSKEKEAY SAWA SNKYTL SSIK SWLKIGDPKNKGKYDRIY NGYGFHLYQD*
PmRXLR1 Δ4 碱基Base	ACTGCGGACTTCGACGGAACTGAAGTGTCCACGATGGAGTCGCCAGACATCGTCCGGCCCTCGCTCAAGAACGACGACATTGCC GGTGGGAGGTTGCTGCGATCCCACCAAGAGGACGACGCGGAGGAACGGACCTTCATCATTACTGAACTGATGAACAAGGC GGCGGTTAAAAAGTTGGCCAGGCAGATGCTGAAGGACCCATCAAAGGAGAAGGAAGCGTACGAGGCGTGGGCA GCGAACAAGTACACCCTG GGCGGTGGAGGGAACTGGCTGAAAATCGGGGACC CCAAGAACAAAGGAAAATATGACCGCATCTACAACGGGTACGGCTTTCACCTGTATCAGGATTAA
PmRXLR1 Δ4 氨基酸Amino acid	TADFDGTEVSTMESPDIVRPSLKNDDIAGGRLLRSHQEDDAEERTFIITE LMNKAAVKKLARQMLKDPSKEKEAYEAWAANKYTLGGGGNWLKIGDPKNKGKYDRIYNGYGFHLYQD*
PmRXLR1 Δ5 碱基Base	ACTGCGGACTTCGACGGAACTGAAGTGTCCACGATGGAGTCGCCAGACATCGTCCGGCCCTCGCTCAAGAACGAC GACATTGCCGGTGGGAGGTTGCTGCGATCCCACCAAGAGGACGACGCGGAGGAACGGACCTTCATCATTACTGAACTGATG AACAAGGCGGCGGTTAAAAAGTTGGCCAGGCAGATGC TGAAGGACCCATCAAAGGAGAAGGAAGCGTACGAGGCGTGGGC AGCGAACAAGTACACCCTGGTCCAA GGCAAGAACTGGCTGAAAATCGGGGACCCCAA GAACAAAGGAAAATATGACCGCATCTACAACGGGTACGGC TTTCACCTGTATCAGGATTAA
PmRXLR1 Δ5 氨基酸Amino acid	TADFDGTEVSTMESPDIVRPSLKNDDIAGGRLLRSHQEDDAE ERTFIITELMNKAAVKKLARQMLKDPSKEKEAYEAWAANKYTLVQ GKNWLKIGDPKNKGK YDRIYNGYGFHLYQD*
PmRXLR1 Δ6 碱基Base	ACTGCGGACTTCGACGGAACTGAAGTGTCCACGATGGAGTCGCCAGACATCGTCCGGCCCTCGCTCAAG AACGACGACATTGCCGGTGGGAGGTTGCTGCGATCCCACCAAGAGGACGACGCGGAGGAACGGACCTTCATCATTACTGAACTGATGAACAAGGCGGCGGTT AAAAAGTTGGCCAGGCAGATGCTGAAGGACCCATCAAAGGAGAAGGAAGCGTACGAGGCGTGGGCAGCGAACAAGTACACCCTGGTCCAAATCAAGAACTGGCT GAAAATCGGGGACCCCAAG---GATTAA
PmRXLR1 Δ6 氨基酸Amino acid	TADFDGTEVSTMESPDIVRPSLKNDDIAGGRLLRSHQEDDAEERTFIITELMNK AAVKKLARQMLKDPSKEKEAYEAWAANKYTLVQIKNWLKIGDPK---D*
PmRXLR1 Δ7 碱基Base	ACTGCGGACTTCGACGGAACTGAAGTGTCCACGATGGAGTCGCCAGACATCGTCCGGCCCTCGCTCAAGAACGACG ACATTGCCGGTGGGAGGTTGCTGCGATCCCACCAAGAGGACGACGCGGAGGAACGGACCTTCATCATTACTGAACTGATGAACAAGGCGGCGGTTAAAAAGTTGGCC AGGCAGATGCTGAAGGACCCATCAAAGGAGA AGGAAGCGTACGAGGCGTGGGCAGCGAACAAGTACACCCTGGTCCAAATCAAGAACTGGCTGAAAATCGGGGACCCCAAG AACAAAGGAGCAGCTGCCGCCATCTACGCCGCGTACGGCG CTGCCCTGTATCAGGATTAA
PmRXLR1 Δ7 氨基酸Amino acid	TADFDGTEVSTMESPDIVRPSLKNDDIAGGRLLRSHQEDD AEERTFIITELMNKAAVKKLARQMLKDPSKEKEAY EAWAANKYTLVQIKNWLKIGDPKNKGAAAAIYAAYGAALYQD*

CsPUB54 Negatively Regulates Cucumber Resistance to Phytophthora Melonis by Interacting with PmRXLR1 Effector

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