Identification of VaSR Gene Family in Vitis amurensis，Verification of Cold Resistance Function of VaSR1 and Screening of Interacting Proteins

doi:10.16420/j.issn.0513-353x.2024-0109

Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (1): 37-50.doi: 10.16420/j.issn.0513-353x.2024-0109

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles

Identification of VaSR Gene Family in Vitis amurensis，Verification of Cold Resistance Function of VaSR1 and Screening of Interacting Proteins

LIANG Guoping, ZENG Baozhen, LIU Ming, BIAN Zhiyuan, CHEN Baihong, MAO Juan^*()

College of Horticulture，Gansu Agricultural University，Lanzhou 730070，China

Received:2024-03-17 Revised:2024-07-26 Online:2025-01-25 Published:2024-11-22
Contact: MAO Juan

Abstract

Abstract:

To investigate the cold-resistance function of serine/arginine-rich splicing factor（SR）in grapevine，the present study selected VaSR1 from the transcriptome data of the phloem of branches treated with various temperature periods during the low-temperature dormancy period of Vitis amurensis Rupr.，and performed cloning，identification of cold-resistant functions，and screening of interacting proteins. Thirteen VaSR family members with the conserved functional domain RRM_SF superfamily were identified from the genome database of V. amurensis. Phylogenetic and collinearity analysis revealed that the majority of the members of the VaSRs were clustered into the SC subfamily and were closely related to the SR proteins of apple. The cold tolerance function of VaSR1 was verified，and the freezing survival rate of transgenic Arabidopsis plants was significantly lower than that of the wild type；the soluble sugar content and the activities of antioxidant enzymes and amylase in the leaf of transgenic plants were significantly lower than those of the wild type after 4 ℃ cold stress，while the starch content was significantly increased；the yeast two-hybridization elucidated that VaU1SNRNP and VaCYP63 were the intercalating proteins of VaSR1 with the ability to promote precursor mRNA splicing and involved in regulating polyadenylation of related genes in cells. Collectively，VaSR1 overexpression reduced amylase activity in transgenic plants，leading to a reduction in soluble sugar content in the leaves and attenuating the low-temperature tolerance of the plants. Furthermore，the presence of VaU1SNRNP and VaCYP63 in mountain grapes was capable of interacting with VaSR1.

Key words: Vitis amurensis, transcription factor, VaSR1, cold stress, yeast two-hybrid

LIANG Guoping, ZENG Baozhen, LIU Ming, BIAN Zhiyuan, CHEN Baihong, MAO Juan. Identification of VaSR Gene Family in Vitis amurensis，Verification of Cold Resistance Function of VaSR1 and Screening of Interacting Proteins[J]. Acta Horticulturae Sinica, 2025, 52(1): 37-50.

Figures/Tables 11

References 30

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基因 Gene	基因ID Gene ID	基因全长/ bp Gene length	CDS全长/ bp CDS length	氨基酸残基数 Amino acid residue number	相对分子量 Molecular weight	等电点 pI	不稳定指数 Instability index	亲水性 Hydropathicity index
VaSR1	VIT_209s0002g06810	1 599	921	307	32 952.0	4.70	55.38	-0.61
VaSR45A	VIT_200s0264g00120	3 478	783	261	30 353.1	11.50	87.59	49.50
VaSR45A-like	VIT_202s0025g00090	3 345	732	244	27 996.2	11.70	101.00	-1.23
VaSR45B	VIT_206s0004g06010	2 145	993	331	38 616.9	11.80	133.80	-1.42
VaRS31	VIT_215s0046g00050	2 580	726	242	28 559.7	9.80	74.15	-1.15
VaRS41	VIT_216s0100g00450	3 441	1 437	479	55 565.7	10.50	90.20	-1.65
VaRS41-like	VIT_215s0048g01870	3 980	924	308	35 855.2	9.90	63.80	-0.96
VaRSZ2-like	VIT_204s0069g00800	4 085	549	183	20 726.9	11.40	104.10	-1.34
VaRSZ22A	VIT_201s0026g00250	8 907	540	180	19 863.1	11.30	93.50	-1.13
VaRSZ32	VIT_208s0040g02860	2 897	801	267	30 402.1	10.52	110.00	-1.72
VaRSZ32-like	VIT_213s0067g03600	2 846	978	326	37 282.2	10.59	90.92	-1.57
VaSC35	VIT_218s0001g05550	2 089	774	258	29 815.9	11.26	94.78	-1.62
VaSC35-like	VIT_212s0142g00110	6 748	768	256	29 827.9	11.03	82.21	-1.40

基因 Gene	基因ID Gene ID	基因全长/ bp Gene length	CDS全长/ bp CDS length	氨基酸残基数 Amino acid residue number	相对分子量 Molecular weight	等电点 pI	不稳定指数 Instability index	亲水性 Hydropathicity index
VaSR1	VIT_209s0002g06810	1 599	921	307	32 952.0	4.70	55.38	-0.61
VaSR45A	VIT_200s0264g00120	3 478	783	261	30 353.1	11.50	87.59	49.50
VaSR45A-like	VIT_202s0025g00090	3 345	732	244	27 996.2	11.70	101.00	-1.23
VaSR45B	VIT_206s0004g06010	2 145	993	331	38 616.9	11.80	133.80	-1.42
VaRS31	VIT_215s0046g00050	2 580	726	242	28 559.7	9.80	74.15	-1.15
VaRS41	VIT_216s0100g00450	3 441	1 437	479	55 565.7	10.50	90.20	-1.65
VaRS41-like	VIT_215s0048g01870	3 980	924	308	35 855.2	9.90	63.80	-0.96
VaRSZ2-like	VIT_204s0069g00800	4 085	549	183	20 726.9	11.40	104.10	-1.34
VaRSZ22A	VIT_201s0026g00250	8 907	540	180	19 863.1	11.30	93.50	-1.13
VaRSZ32	VIT_208s0040g02860	2 897	801	267	30 402.1	10.52	110.00	-1.72
VaRSZ32-like	VIT_213s0067g03600	2 846	978	326	37 282.2	10.59	90.92	-1.57
VaSC35	VIT_218s0001g05550	2 089	774	258	29 815.9	11.26	94.78	-1.62
VaSC35-like	VIT_212s0142g00110	6 748	768	256	29 827.9	11.03	82.21	-1.40

互作蛋白 Interacting proteins	互作蛋白ID Interacting proteins ID	基因长度/bp Gene length	氨基酸数 Amino acid residue number	互作蛋白功能结构域 Interacting proteins function domain	互作蛋白功能描述 Interacting proteins character
U1SNRNP （尿苷酸小分子1核糖体蛋白-70K U1 small nuclear ribonucleoprotein 70 kD）	XP_010649159	9 406	514	RRM_snRNP70 U1snRNP70_N PRK12678 superfamily	促进前体mRNA的剪接，并参与调节细胞中基因的聚腺苷酸化位点数 Promotes splicing of precursor mRNA and involved in regulating the polyadenylation site number of genes in cells
CYP63 （肽基—丙酰基顺式转运异构酶CYP63 Peptidyl- prolyl cis-trans isomerase）	RVX20698	6 800	755	肽基丙酰异构酶结构域Peptidyl propionyl isomerase domain（PpiB）	催化寡肽中脯氨酸酰亚胺肽键的顺反异构化作用Catalyze the cis-trans isomerization of proline imide peptide bonds in oligo- peptides

互作蛋白 Interacting proteins	互作蛋白ID Interacting proteins ID	基因长度/bp Gene length	氨基酸数 Amino acid residue number	互作蛋白功能结构域 Interacting proteins function domain	互作蛋白功能描述 Interacting proteins character
U1SNRNP （尿苷酸小分子1核糖体蛋白-70K U1 small nuclear ribonucleoprotein 70 kD）	XP_010649159	9 406	514	RRM_snRNP70 U1snRNP70_N PRK12678 superfamily	促进前体mRNA的剪接，并参与调节细胞中基因的聚腺苷酸化位点数 Promotes splicing of precursor mRNA and involved in regulating the polyadenylation site number of genes in cells
CYP63 （肽基—丙酰基顺式转运异构酶CYP63 Peptidyl- prolyl cis-trans isomerase）	RVX20698	6 800	755	肽基丙酰异构酶结构域Peptidyl propionyl isomerase domain（PpiB）	催化寡肽中脯氨酸酰亚胺肽键的顺反异构化作用Catalyze the cis-trans isomerization of proline imide peptide bonds in oligo- peptides

Identification of VaSR Gene Family in Vitis amurensis，Verification of Cold Resistance Function of VaSR1 and Screening of Interacting Proteins

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