Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (3): 495-507.doi: 10.16420/j.issn.0513-353x.2021-1282
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LIU Yunuo*, CAO Ya*, WANG Shuai, DU Meixia, ZHENG Lin, CHEN Shanchun, ZOU Xiuping**()
Received:
2022-08-23
Revised:
2022-12-21
Online:
2023-03-25
Published:
2023-04-03
Contact:
**(E-mail:zouxiuping@cric.cn)
CLC Number:
LIU Yunuo, CAO Ya, WANG Shuai, DU Meixia, ZHENG Lin, CHEN Shanchun, ZOU Xiuping. Expression Analysis of CsMYB41 and CsMYB63 Genes in Response to Citrus Canker[J]. Acta Horticulturae Sinica, 2023, 50(3): 495-507.
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URL: https://www.ahs.ac.cn/EN/10.16420/j.issn.0513-353x.2021-1282
引物名称 Primer | 序列(5′-3′) Sequences | 引物名称 Primer | 序列(5′-3′) Sequences |
---|---|---|---|
qCs1g06220-F | AAGCTTGTCGAGTAATTGAAATGGG | qCs1g06220-R | TTATTATACAAGTCCAATCGCATCC |
qCs4g12760-F | ATTATCTCAAATGGGAAAAGGGAGA | qCs4g12760-R | AATTATTTGATCACTCGCAAACGGA |
Cs1g06220-F | CGGGGTACC ATGGGAAGGCCACCAAGCTC | Cs1g06220-R | CGCGGATCC TACAAGTCCAATCGCATCCA |
Cs4g12760-F | CGGGGTACC ATGGGAAAAGGGAGAGCTCC | Cs4g12760-R | CGCGGATCC CTCGCAAACGGAACTTTGTA |
Actin-F | CATCCCTCAGCACCTTCC | Actin-R | CCAACCTTAGCACTTCTCC |
Table 1 Primers used in this study
引物名称 Primer | 序列(5′-3′) Sequences | 引物名称 Primer | 序列(5′-3′) Sequences |
---|---|---|---|
qCs1g06220-F | AAGCTTGTCGAGTAATTGAAATGGG | qCs1g06220-R | TTATTATACAAGTCCAATCGCATCC |
qCs4g12760-F | ATTATCTCAAATGGGAAAAGGGAGA | qCs4g12760-R | AATTATTTGATCACTCGCAAACGGA |
Cs1g06220-F | CGGGGTACC ATGGGAAGGCCACCAAGCTC | Cs1g06220-R | CGCGGATCC TACAAGTCCAATCGCATCCA |
Cs4g12760-F | CGGGGTACC ATGGGAAAAGGGAGAGCTCC | Cs4g12760-R | CGCGGATCC CTCGCAAACGGAACTTTGTA |
Actin-F | CATCCCTCAGCACCTTCC | Actin-R | CCAACCTTAGCACTTCTCC |
基因ID Gene ID | 基因描述 Gene description | 表达差异倍数 log2 fold change | |||||
---|---|---|---|---|---|---|---|
纽荷尔脐橙 Newhall Navel Orange | 四季橘 Calamondin | ||||||
1 d | 3 d | 5 d | 1 d | 3 d | 5 d | ||
Cs1g01670 | MYB93一种新的侧根发育负调控因子 MYB93 a novel negative regulator of lateral root development | 0.0 | 0.0 | 0.0 | 0.0 | 3.6 | 4.7 |
Cs1g06220 | MYB41参与创伤和渗透应激反应 MYB41 involved in wounding and osmotic stress response | 0.0 | 4.5 | 4.7 | 0.0 | 0.0 | 0.0 |
Cs1g19400 | MYB7参与原花青素的合成 MYB7 involved in proanthocyanidins synthesis | 2.5 | 4.0 | 6.1 | 1.1 | 0.4 | 3.3 |
Cs1g19970 | MYB78响应脱落酸和盐胁迫 MYB78 responses to abscisic acid and salt stress | 4.3 | 4.4 | 5.5 | 3.0 | 3.9 | 4.7 |
Cs2g06950 | MYB样蛋白102参与创伤和渗透应激 MYB-like 102 involved in wounding and osmotic stress | 3.4 | 5.1 | 7.1 | 3.1 | 2.3 | 2.8 |
Cs3g02020 | MYB同源结构域样超家族蛋白 MYB Homeodomain-like superfamily protein | 2.2 | 3.4 | 5.2 | 1.0 | 1.0 | 1.8 |
Cs3g22220 | MYB6参与调节花青素生物合成 MYB6 involved in regulating anthocyanin biosynthesis | 3.2 | 4.0 | 5.0 | 2.5 | 3.0 | 4.3 |
Cs3g23070 | MYBR1参与多种非生物刺激反应 MYBR1 involved in a variety of abiotic stimiuli response | 1.9 | 3.3 | 3.8 | 1.3 | 1.9 | 2.5 |
Cs4g07780 | MYB103是花药发育所必需的 MYB103 is required for anther development | 4.0 | 3.4 | 5.4 | 2.6 | 3.7 | 4.9 |
Cs4g12760 | MYB63参与细胞壁发育 MYB63 involved in cell wall development | 8.5 | 10.3 | 11.2 | 6.2 | 5.9 | 7.5 |
Cs4g18910 | MYB36参与侧根边界的发育 MYB36 involved in develompent of lateral root boundaries | 1.0 | -0.5 | -0.8 | 1.2 | 2.0 | 2.4 |
Cs5g10670 | MYB96在干旱胁迫期间介导脱落酸信号传导 MYB96 mediates abscisic acid signaling during drought stress | -1.5 | -1.4 | -8.1 | -0.9 | -1.2 | -3.4 |
Cs5g26420 | 同源结构域样超家族蛋白 Homeodomain-like superfamily protein | -1.1 | -1.9 | -3.8 | -0.5 | -0.8 | -2.1 |
Cs5g27440 | MYB117在器官模式中发挥功能 MYB117 functions in organ patterning | 2.0 | 1.1 | -0.8 | 1.7 | 1.1 | 0.7 |
Cs5g29830 | MYB14 | 2.1 | 0.6 | 1.1 | 3.6 | 2.1 | 1.5 |
Cs6g17340 | MYB20 | 4.2 | 2.4 | 0.6 | 3.8 | 2.8 | 1.4 |
Cs7g26930 | MYB62参与赤霉素生物合成的调节 MYB62 involved in regulation of gibberellic acid biosynthesis | 3.0 | 3.7 | 4.0 | 1.9 | 1.6 | 2.0 |
Cs7g32400 | TBP1具有单个MYB端粒DNA结合域 TBP1 with a single MYB telomeric DNA-binding domain | 0.2 | 0.8 | 3.2 | 0.0 | 0.0 | 1.0 |
Cs8g12680 | MYB4相关蛋白 MYB-related protein MYB4 | 1.5 | 2.1 | 2.6 | 2.5 | 2.5 | 2.2 |
Cs9g01750 | 推测转录因子MYB108 putative transcription factor MYB108 | 2.1 | 5.0 | 6.1 | 1.9 | 2.5 | 4.7 |
Orange1.1t00897 | MYB38调节腋生分生组织的形成 MYB38 regulates axillary meristem formation | 2.9 | 4.2 | 5.6 | 2.4 | 3.9 | 3.7 |
Table 2 Differentially expressed MYB genes responding to Xcc infection
基因ID Gene ID | 基因描述 Gene description | 表达差异倍数 log2 fold change | |||||
---|---|---|---|---|---|---|---|
纽荷尔脐橙 Newhall Navel Orange | 四季橘 Calamondin | ||||||
1 d | 3 d | 5 d | 1 d | 3 d | 5 d | ||
Cs1g01670 | MYB93一种新的侧根发育负调控因子 MYB93 a novel negative regulator of lateral root development | 0.0 | 0.0 | 0.0 | 0.0 | 3.6 | 4.7 |
Cs1g06220 | MYB41参与创伤和渗透应激反应 MYB41 involved in wounding and osmotic stress response | 0.0 | 4.5 | 4.7 | 0.0 | 0.0 | 0.0 |
Cs1g19400 | MYB7参与原花青素的合成 MYB7 involved in proanthocyanidins synthesis | 2.5 | 4.0 | 6.1 | 1.1 | 0.4 | 3.3 |
Cs1g19970 | MYB78响应脱落酸和盐胁迫 MYB78 responses to abscisic acid and salt stress | 4.3 | 4.4 | 5.5 | 3.0 | 3.9 | 4.7 |
Cs2g06950 | MYB样蛋白102参与创伤和渗透应激 MYB-like 102 involved in wounding and osmotic stress | 3.4 | 5.1 | 7.1 | 3.1 | 2.3 | 2.8 |
Cs3g02020 | MYB同源结构域样超家族蛋白 MYB Homeodomain-like superfamily protein | 2.2 | 3.4 | 5.2 | 1.0 | 1.0 | 1.8 |
Cs3g22220 | MYB6参与调节花青素生物合成 MYB6 involved in regulating anthocyanin biosynthesis | 3.2 | 4.0 | 5.0 | 2.5 | 3.0 | 4.3 |
Cs3g23070 | MYBR1参与多种非生物刺激反应 MYBR1 involved in a variety of abiotic stimiuli response | 1.9 | 3.3 | 3.8 | 1.3 | 1.9 | 2.5 |
Cs4g07780 | MYB103是花药发育所必需的 MYB103 is required for anther development | 4.0 | 3.4 | 5.4 | 2.6 | 3.7 | 4.9 |
Cs4g12760 | MYB63参与细胞壁发育 MYB63 involved in cell wall development | 8.5 | 10.3 | 11.2 | 6.2 | 5.9 | 7.5 |
Cs4g18910 | MYB36参与侧根边界的发育 MYB36 involved in develompent of lateral root boundaries | 1.0 | -0.5 | -0.8 | 1.2 | 2.0 | 2.4 |
Cs5g10670 | MYB96在干旱胁迫期间介导脱落酸信号传导 MYB96 mediates abscisic acid signaling during drought stress | -1.5 | -1.4 | -8.1 | -0.9 | -1.2 | -3.4 |
Cs5g26420 | 同源结构域样超家族蛋白 Homeodomain-like superfamily protein | -1.1 | -1.9 | -3.8 | -0.5 | -0.8 | -2.1 |
Cs5g27440 | MYB117在器官模式中发挥功能 MYB117 functions in organ patterning | 2.0 | 1.1 | -0.8 | 1.7 | 1.1 | 0.7 |
Cs5g29830 | MYB14 | 2.1 | 0.6 | 1.1 | 3.6 | 2.1 | 1.5 |
Cs6g17340 | MYB20 | 4.2 | 2.4 | 0.6 | 3.8 | 2.8 | 1.4 |
Cs7g26930 | MYB62参与赤霉素生物合成的调节 MYB62 involved in regulation of gibberellic acid biosynthesis | 3.0 | 3.7 | 4.0 | 1.9 | 1.6 | 2.0 |
Cs7g32400 | TBP1具有单个MYB端粒DNA结合域 TBP1 with a single MYB telomeric DNA-binding domain | 0.2 | 0.8 | 3.2 | 0.0 | 0.0 | 1.0 |
Cs8g12680 | MYB4相关蛋白 MYB-related protein MYB4 | 1.5 | 2.1 | 2.6 | 2.5 | 2.5 | 2.2 |
Cs9g01750 | 推测转录因子MYB108 putative transcription factor MYB108 | 2.1 | 5.0 | 6.1 | 1.9 | 2.5 | 4.7 |
Orange1.1t00897 | MYB38调节腋生分生组织的形成 MYB38 regulates axillary meristem formation | 2.9 | 4.2 | 5.6 | 2.4 | 3.9 | 3.7 |
Fig. 2 Alignment of the CsMYB41 and CsMYB63 amino acid sequences with MYB transcription factors from other species Underlines indicate nuclear localization signal sequence.
Fig. 3 Phylogenetic analysis of deduced amino acid sequences of MYB from different plants The ruler represents the unit length of the difference value between different plant sequences;The number represents the length of the evolutionary branch.
Fig. 5 The expression characteristics of CsMYB41 and CsMYB63 genes in citrus leaves infected by Xanthomonas citri subsp. citri(Xcc) Different letters on the top of bar represented significant differences compared to the 0 d treatment(Tukey’s test,P < 0.05).
Fig. 6 CsMYB41 expression induced by exogenous plant growth regulators Different letters on the top of bar represented significant differences compared to the 0 h treatment(Tukey’s test,P < 0.05). The same below.
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