Genome-wide Identification of GASA Family and Preliminary Exploration of Leaf Variegation Formation in Begonia masoniana

doi:10.16420/j.issn.0513-353x.2022-0472

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (7): 1482-1494.doi: 10.16420/j.issn.0513-353x.2022-0472

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles

Genome-wide Identification of GASA Family and Preliminary Exploration of Leaf Variegation Formation in Begonia masoniana

XUE Zhenzhen¹, GUAN Hongfa³, LI Na², LI Lingfei²^,^*(), ZHONG Chunmei¹^,^*()

¹ Key Laboratory of Energy Plants Resource and Utilization，Ministry of Agriculture and Rural Affairs，Guangdong Engineering Technology Research Center of Agricultural and Forestry Biomass，Institute of Biomass Engineering，South China Agricultural University，Guangzhou 510642，China
² Key Laboratory of Southern Subtropical Plant Diversity，Fairy Lake Botanical Garden，Shenzhen & Chinese Academy of Sciences，Shenzhen，Guangdong 518004，China
³ College of Life and Science，South China Agricultural University，Guangzhou 510642，China

Received:2023-02-26 Revised:2023-05-15 Online:2023-07-25 Published:2023-07-26
Contact: LI Lingfei, ZHONG Chunmei

Abstract

Abstract:

In order to understand the potential functions of Gibberellic Acid-stimulated Arabidopsis （GASA）in Begonia masoniana（BmaGASAs），the bioinformatics analysis was carried out to identify the members of BmaGASA gene family，and then their expression patterns were analyzed. The results showed that ten members of the GASA family were distributed in four subgroups. The structural characteristics of BmaGASAs include 2-3 motifs，one GASA conserved domain，0-10 introns that varies among different members. The cis-acting elements distribution analysis showed that the BmaGASAs promoters contained abundant light and hormone response elements. BmaGASAs were widely expressed in various tissues and organs. In addition，BmaGASAs had significant differences in response to GA，ABA，SA，MeJA and auxin. This study indicates that members of the BmaGASA gene family are highly conserved and have diverse expression patterns. They may be involved in the growth and development of various tissues and organs，as well as the response processes of various biological or abiotic stress. Among them，BmaGASA7 may play an important role in leaf variegation formation.

Key words: Begonia masoniana, GASA, hormonal response, expression analysis, leaf variegation

XUE Zhenzhen, GUAN Hongfa, LI Na, LI Lingfei, ZHONG Chunmei. Genome-wide Identification of GASA Family and Preliminary Exploration of Leaf Variegation Formation in Begonia masoniana[J]. Acta Horticulturae Sinica, 2023, 50(7): 1482-1494.

Figures/Tables 7

Table 2 Basic physicochemical property of BmGASA family

基因 Gene	CDS/bp	氨基酸数 Amino acid number	等电点 pI	分子量/kD Molecular weight	染色体位置 Chr.	注释到拟南芥中 Annotated members of Arabidopsis thaliana	糖基化位点 N-glycosyl sites	亚细胞定位 Subcellular location
BmaGASA1	255	84	8.42	9.28	Chr01	GASA10	0	细胞壁 Cytoderm
BmaGASA2	432	143	9.40	15.13	Chr01	GASA14	0	细胞核 Nucleus
BmaGASA3	330	109	9.46	12.20	Chr01	GASA4	1	线粒体 Mitochondria
BmaGASA4	201	66	8.95	7.55	Chr02	GASA4	0	细胞核 Nucleus
BmaGASA5	309	102	9.18	10.74	Chr03	GASA1	0	细胞壁 Cytoderm
BmaGASA6	240	79	9.66	6.73	Chr04	GASA1	0	细胞核 Nucleus
BmaGASA7	183	60	9.20	6.50	Chr06	GASA1	1	线粒体 Mitochondria
BmaGASA8	270	89	9.26	10.12	Chr08	GASA7	0	细胞壁 Cytoderm
BmaGASA9	195	64	9.05	7.27	Chr13	GASA5	0	线粒体 Mitochondria
BmaGASA10	183	60	9.14	6.65	Chr14	GASA6	0	线粒体 Mitochondria

Fig. 1 Chromosome location of BmaGASA family genes

Fig. 2 Phylogenetic tree of GASA family members of Arabidopsis thaliana（At），Glycine max（Gm），Malus × domestica（Md）and Begonia masoniana（Bma）

Fig. 3 Genetic structure（A），conserved motif distribution（B），and conserved domain distribution（C）of BmaGASA family

Fig. 4 Number and type of cis-acting elements in GASA family promoter sequence of Begonia masoniana

Fig. 5 Relative expression levels of GASA gene family in different tissues and organs of Begonia masoniana The reference gene was ACT7，and the relative gene expression was shown as the mean ± SD. The red part of the leaf is the leaf variegation part. The minimum expression of BamGASAs in different tissues was regarded as“1”.

Fig. 6 Relative expression levels of GASA gene family in Begonia masoniana under GA, ABA, SA, MeJA and NAA treatments（6 h） The relative expression of control is regarded as“1”. *，** and *** represent significant differences at the levels of 0.05，0.01 and 0.001 respectively（t-test）.

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Genome-wide Identification of GASA Family and Preliminary Exploration of Leaf Variegation Formation in Begonia masoniana

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