Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (5): 1060-1072.doi: 10.16420/j.issn.0513-353x.2021-0673

• Research Papers • Previous Articles     Next Articles

Identification of Viruses Infecting Peppers in Guangxi by Small RNA Deep Sequencing and RT-PCR

GONG Mingxia1, ZHAO Hu1, WANG Meng1, WU Xing1, ZHAO Zengjing1, HE Zhi1, HUANG Jinmei1, MENG Shengde2, WANG Risheng1,*()   

  1. 1Guangxi Key Laboratory of Vegetable Breeding and New Technology Development,Vegetable Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,China
    2Gansu Dunhuang Seed Industry Group Co. Ltd,Jiuquan,Gansu 735008,China
  • Received:2022-03-02 Revised:2022-04-22 Online:2022-05-25 Published:2022-05-25
  • Contact: WANG Risheng


We analysed diseased pepper samples with typical viral symptoms collected from nine of major pepper growing areas in Guangxi through small RNA(sRNA)deep sequencing technology and RT-PCR. The results showed that ten viruses were identified,among which,Capsicum frutescens endornavirus 1(CFEV 1),pepper cryptic virus 2(PCV 2),tobacco mild green mosaic virus(TMGMV)and pepper vein yellows virus(PeVYV)were detected on pepper for the first time in Guangxi,and CFEV 1 was also detected for the first time in China. The total detection rates of ten viruses in 75 diseased pepper samples were 8.00%-66.67%. The top five viruses were chilli veinal mottle virus(ChiVMV,66.67%),pepper veinal mottle virus(PVMV,62.67%),cucumber mosaic virus(CMV,41.33%),CFEV 1(32.0%)and PCV 2(26.67%). ChiVMV,PVMV,CMV and CFEV 1 were found to be widely distributed across seven to nine of the major pepper growing areas of Guangxi,which were speculated to be the dominant viruses infecting peppers in Guangxi. Except Guilin,other eight regions contained at least one to three of the four dominant viruses. However,in Guilin,the detection rates of these four dominant viruses were relatively low or negligible,and the dominant viruses were PMMoV and PCV 2. The mixed infection rate of pepper virus disease was up to 94.66% in Guangxi,and the mixed infection were mostly caused by a complex of three(detection rate 42.67%)or four viruses(detection rate 24.00%)in Guangxi. Mixed infection of three viruses were predominantly caused by ChiVMV + PVMV + CMV and ChiVMV + CFEV 1 + ChiRSV both with detection rate of 18.75%,that of four viruses was mostly caused by ChiVMV + PVMV + CMV + CFEV 1 with detection rate of 16.67%.

Key words: pepper, viral diseases, small RNA, deep sequencing, RT-PCR, molecular detection

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