Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (4): 893-906.doi: 10.16420/j.issn.0513-353x.2021-0051
• Research Notes • Previous Articles Next Articles
GAO Xiaomei1,*(), LÜ Guozhong2, LI Yang1, AO Jing1, LIU Xiaohui1, SUN Yulu1
Received:
2021-06-15
Revised:
2021-11-16
Online:
2022-04-25
Published:
2022-04-25
Contact:
GAO Xiaomei
E-mail:gaoxiaomei8115@163.com
CLC Number:
GAO Xiaomei, LÜ Guozhong, LI Yang, AO Jing, LIU Xiaohui, SUN Yulu. Identification of the Pathogens Causing Root Rot on Allium tuberosum and Study on Their Biological Characteristics[J]. Acta Horticulturae Sinica, 2022, 49(4): 893-906.
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URL: https://www.ahs.ac.cn/EN/10.16420/j.issn.0513-353x.2021-0051
Fig. 2 The different basal bulb of Allium tuberosum on root rot and bulb rot a,b:Basal bulb of root rot;c,d:Basal bulb of bulb rot(Yamazaki et al.,2013);e:Basal bulb of healthy root.
Fig. 3 Morphological characteristics of Fusarium oxysporum a:Colony on PDA(surface and bottom);b:Conidiophore;c:Microconidia; d:Macroconidia in orange slime.
Fig. 4 Morphological characteristics of Fusarium proliferatum a:Colony on PDA(surface and bottom);b:Conidiophore and microconidia;c:Microconidia in chain;d:Microconidia and macroconidia.
Fig. 5 Morphological characteristics of Fusarium solani a:Colony on PDA(surface and bottom);b:Conidiophore and conidia;c:Microconidia and macroconidia.
病原菌Pathogens | 发病率/% Morbidity | 病情指数Disease index |
---|---|---|
尖孢镰孢菌F. oxysporum | 96.88 ± 1.46 ab | 87.01 ± 1.79 a |
层出镰孢菌F. proliferatum | 95.71 ± 1.21 b | 80.83 ± 2.08 b |
腐皮镰孢菌F. solani | 98.90 ± 0.33 a | 86.18 ± 1.51 a |
Table 1 The pathogenicity of three Fusarium species to chive root
病原菌Pathogens | 发病率/% Morbidity | 病情指数Disease index |
---|---|---|
尖孢镰孢菌F. oxysporum | 96.88 ± 1.46 ab | 87.01 ± 1.79 a |
层出镰孢菌F. proliferatum | 95.71 ± 1.21 b | 80.83 ± 2.08 b |
腐皮镰孢菌F. solani | 98.90 ± 0.33 a | 86.18 ± 1.51 a |
试验 Test | 培养条件 Culture condition | 菌落直径/mm Colony diameter | ||
---|---|---|---|---|
尖孢镰孢菌 F. oxysporum | 层出镰孢菌 F. proliferatum | 腐皮镰孢菌 F. solani | ||
碳源Carbon source | 蔗糖Sucrose | 83.94 ± 0.50 a | 69.86 ± 1.78 c | 57.75 ± 0.40 c |
菊糖Inulin | 83.85 ± 1.20 a | 76.21 ± 0.53 b | 58.07 ± 0.22 c | |
葡萄糖Glucose | 84.64 ± 2.39 a | 67.48 ± 1.03 c | 56.66 ± 0.15 c | |
山梨糖Sorbose | 68.96 ± 0.76 c | 53.79 ± 1.98 e | 51.26 ± 0.75 d | |
木糖Xylose | 68.71 ± 1.60 c | 62.91 ± 1.77 d | 57.72 ± 1.02 c | |
乳糖Lactose | 83.74 ± 0.50 a | 76.65 ± 1.23 b | 60.61 ± 1.18 b | |
果糖Fructose | 75.96 ± 1.28 b | 66.64 ± 3.28 c | 57.08 ± 2.88 c | |
鼠李糖Rhamnose | 82.53 ± 0.87 a | 82.02 ± 2.36 a | 65.21 ± 1.21 a | |
甘露糖Mannose | 78.38 ± 3.62 b | 74.52 ± 1.07 b | 58.47 ± 0.66 c | |
氮源Nitrogen source | NH4Cl | 77.01 ± 1.71 a | 58.51 ± 1.55 c | 51.01 ± 1.80 d |
NaNO3 | 74.32 ± 0.76 a | 62.74 ± 2.55 b | 59.68 ± 0.49 b | |
KNO3 | 68.42 ± 2.47 bcd | 66.70 ± 1.39 a | 54.82 ± 2.74 c | |
酒石酸铵Ammonium tartrate | 58.06 ± 3.62 e | 48.73 ± 1.55 d | 55.22 ± 1.40 c | |
甘氨酸Glycine | 72.57 ± 1.97 ab | 56.06 ± 0.28 c | 63.22 ± 2.90 a | |
胱氨酸Cystine | 65.15 ± 3.84 d | 65.64 ± 0.81 ab | 59.86 ± 1.54 b | |
丙氨酸Alanine | 66.22 ± 4.71 cd | 56.94 ± 1.32 c | 61.47 ± 2.09 a | |
CK(PDA) | 71.67 ± 2.99 abc | 56.92 ± 4.16 c | 61.40 ± 0.32 a | |
温度/℃ Temperature | 10 | 15.19 ± 0.35 e | 16.04 ± 0.37 f | 12.29 ± 0.32 e |
15 | 42.66 ± 0.77 d | 43.54 ± 0.83 e | 29.66 ± 1.09 d | |
20 | 57.31 ± 2.04 c | 55.81 ± 0.41 d | 43.04 ± 1.56 c | |
25 | 72.46 ± 2.10 b | 71.26 ± 2.07 b | 52.25 ± 1.17 b | |
30 | 78.39 ± 3.83 a | 74.82 ± 1.61 a | 56.86 ± 1.06 a | |
35 | 71.74 ± 1.83 b | 70.66 ± 1.75 b | 54.47 ± 1.83 ab | |
40 | 58.16 ± 3.70 c | 67.06 ± 1.41 c | 45.09 ± 2.41 c | |
试验 Test | 培养条件 Culture condition | 菌落直径/mm Colony diameter | ||
尖孢镰孢菌 F. oxysporum | 层出镰孢菌 F. proliferatum | 腐皮镰孢菌 F. solani | ||
pH | 4 | 41.91 ± 0.91 c | 44.32 ± 0.26 e | 30.95 ± 3.11 e |
5 | 56.01 ± 0.32 b | 55.27 ± 1.54 d | 47.47 ± 1.24 cd | |
6 | 61.13 ± 0.24 a | 72.11 ± 0.28 b | 49.40 ± 0.30 bcd | |
7 | 62.56 ± 2.27 a | 76.07 ± 2.72 a | 51.52 ± 0.78 ab | |
8 | 60.81 ± 4.95 a | 68.36 ± 1.30 c | 54.80 ± 2.09 a | |
9 | 60.75 ± 3.20 a | 67.35 ± 0.13 c | 52.27 ± 1.76 ab | |
10 | 53.74 ± 0.73 b | 65.87 ± 1.08 c | 50.74 ± 1.67 bc | |
11 | 52.46 ± 0.34 c | 44.31 ± 2.18 e | 46.59 ± 3.68 d | |
光照Illumination | 全光照 Full illumination | 70.28 ± 1.44 c | 67.42 ± 3.54 b | 50.17 ± 3.30 b |
全黑暗 Full darkness | 78.34 ± 0.84 a | 75.27 ± 0.81 a | 60.02 ± 1.36 a | |
12 h光照/12 h黑暗 12 h illumination/12 h darkness | 73.28 ± 1.34 b | 70.54 ± 1.51 ab | 56.86 ± 0.86 a |
Table 2 Effects of nutrients and culturing conditions on the mycelium growth of three Fusarium species
试验 Test | 培养条件 Culture condition | 菌落直径/mm Colony diameter | ||
---|---|---|---|---|
尖孢镰孢菌 F. oxysporum | 层出镰孢菌 F. proliferatum | 腐皮镰孢菌 F. solani | ||
碳源Carbon source | 蔗糖Sucrose | 83.94 ± 0.50 a | 69.86 ± 1.78 c | 57.75 ± 0.40 c |
菊糖Inulin | 83.85 ± 1.20 a | 76.21 ± 0.53 b | 58.07 ± 0.22 c | |
葡萄糖Glucose | 84.64 ± 2.39 a | 67.48 ± 1.03 c | 56.66 ± 0.15 c | |
山梨糖Sorbose | 68.96 ± 0.76 c | 53.79 ± 1.98 e | 51.26 ± 0.75 d | |
木糖Xylose | 68.71 ± 1.60 c | 62.91 ± 1.77 d | 57.72 ± 1.02 c | |
乳糖Lactose | 83.74 ± 0.50 a | 76.65 ± 1.23 b | 60.61 ± 1.18 b | |
果糖Fructose | 75.96 ± 1.28 b | 66.64 ± 3.28 c | 57.08 ± 2.88 c | |
鼠李糖Rhamnose | 82.53 ± 0.87 a | 82.02 ± 2.36 a | 65.21 ± 1.21 a | |
甘露糖Mannose | 78.38 ± 3.62 b | 74.52 ± 1.07 b | 58.47 ± 0.66 c | |
氮源Nitrogen source | NH4Cl | 77.01 ± 1.71 a | 58.51 ± 1.55 c | 51.01 ± 1.80 d |
NaNO3 | 74.32 ± 0.76 a | 62.74 ± 2.55 b | 59.68 ± 0.49 b | |
KNO3 | 68.42 ± 2.47 bcd | 66.70 ± 1.39 a | 54.82 ± 2.74 c | |
酒石酸铵Ammonium tartrate | 58.06 ± 3.62 e | 48.73 ± 1.55 d | 55.22 ± 1.40 c | |
甘氨酸Glycine | 72.57 ± 1.97 ab | 56.06 ± 0.28 c | 63.22 ± 2.90 a | |
胱氨酸Cystine | 65.15 ± 3.84 d | 65.64 ± 0.81 ab | 59.86 ± 1.54 b | |
丙氨酸Alanine | 66.22 ± 4.71 cd | 56.94 ± 1.32 c | 61.47 ± 2.09 a | |
CK(PDA) | 71.67 ± 2.99 abc | 56.92 ± 4.16 c | 61.40 ± 0.32 a | |
温度/℃ Temperature | 10 | 15.19 ± 0.35 e | 16.04 ± 0.37 f | 12.29 ± 0.32 e |
15 | 42.66 ± 0.77 d | 43.54 ± 0.83 e | 29.66 ± 1.09 d | |
20 | 57.31 ± 2.04 c | 55.81 ± 0.41 d | 43.04 ± 1.56 c | |
25 | 72.46 ± 2.10 b | 71.26 ± 2.07 b | 52.25 ± 1.17 b | |
30 | 78.39 ± 3.83 a | 74.82 ± 1.61 a | 56.86 ± 1.06 a | |
35 | 71.74 ± 1.83 b | 70.66 ± 1.75 b | 54.47 ± 1.83 ab | |
40 | 58.16 ± 3.70 c | 67.06 ± 1.41 c | 45.09 ± 2.41 c | |
试验 Test | 培养条件 Culture condition | 菌落直径/mm Colony diameter | ||
尖孢镰孢菌 F. oxysporum | 层出镰孢菌 F. proliferatum | 腐皮镰孢菌 F. solani | ||
pH | 4 | 41.91 ± 0.91 c | 44.32 ± 0.26 e | 30.95 ± 3.11 e |
5 | 56.01 ± 0.32 b | 55.27 ± 1.54 d | 47.47 ± 1.24 cd | |
6 | 61.13 ± 0.24 a | 72.11 ± 0.28 b | 49.40 ± 0.30 bcd | |
7 | 62.56 ± 2.27 a | 76.07 ± 2.72 a | 51.52 ± 0.78 ab | |
8 | 60.81 ± 4.95 a | 68.36 ± 1.30 c | 54.80 ± 2.09 a | |
9 | 60.75 ± 3.20 a | 67.35 ± 0.13 c | 52.27 ± 1.76 ab | |
10 | 53.74 ± 0.73 b | 65.87 ± 1.08 c | 50.74 ± 1.67 bc | |
11 | 52.46 ± 0.34 c | 44.31 ± 2.18 e | 46.59 ± 3.68 d | |
光照Illumination | 全光照 Full illumination | 70.28 ± 1.44 c | 67.42 ± 3.54 b | 50.17 ± 3.30 b |
全黑暗 Full darkness | 78.34 ± 0.84 a | 75.27 ± 0.81 a | 60.02 ± 1.36 a | |
12 h光照/12 h黑暗 12 h illumination/12 h darkness | 73.28 ± 1.34 b | 70.54 ± 1.51 ab | 56.86 ± 0.86 a |
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