Expression Analysis of Genes Related to Photosensitive Color Under the Caylx in Eggplant Based on Transcriptome Sequencing

doi:10.16420/j.issn.0513-353x.2021-0665

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (11): 2347-2356.doi: 10.16420/j.issn.0513-353x.2021-0665

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Expression Analysis of Genes Related to Photosensitive Color Under the Caylx in Eggplant Based on Transcriptome Sequencing

QIAO Jun¹^,³, WANG Liying²^,³^,^*(), LIU Jing¹^,³, LI Suweng²^,³

¹Vegetable Research Institute of Tianjin Kernel Agricultural Technology Co.，Ltd，Tianjin 300381，China
²Institue of Vegetables，Tianjin Academy of Agriculture Science，Tianjin 300381，China
³State Key Laboratory of Vegetable Germplasm Innovation，Tianjin Enterprise Key Laboratory of Vegetable Genetics & Breeding，Tianjin 300381，China

Received:2022-06-04 Revised:2022-09-01 Online:2022-11-25 Published:2022-11-25
Contact: WANG Liying E-mail:sun_hwg@163.com

Abstract

Abstract:

In order to explore the different genes related to low light tolerance between the the photosensitive and non-photosensitive varieties，stable genetic purple under the calyx，round eggplant inbred line Y₅ and green under the calyx，round eggplant inbred line Y₇₃ were sequenced at three-time points（0，4 and 8 h）. The total effective data of 75.61 Gb was obtained，and the percentage of Q30 base reached 93.97%. 11 812 differential expression genes were obtained by sequencing and comparing two inbred lines at three-time points，including 5 277 up-regulated genes and 6 535 down-regulated genes. KEGG enrichment analysis of data at three time points shows three same pathways of phenylpropane biosynthesis，flavonoids biosynthesisand carbon fixation of photosynthetic organisms. There are four genes in the biosynthesis pathway of phenylpropane and flavonoids，named FAOMT，CCOAOMT，CYP73A4 and HST. The CHI，ANS，F3H，PAL genes and the transcription factor MYB113 gene related to anthocyanin synthesis were screened to express significantly in the peel under the calyx.

Key words: eggplant, transcriptome sequencing, color under the caylx, photosensitive, gene expression

CLC Number:

S641.1

QIAO Jun, WANG Liying, LIU Jing, LI Suweng. Expression Analysis of Genes Related to Photosensitive Color Under the Caylx in Eggplant Based on Transcriptome Sequencing[J]. Acta Horticulturae Sinica, 2022, 49(11): 2347-2356.

Figures/Tables 6

References 31

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样品 Sample	见光时间/h Light exposure time	重复 Repeat	过滤后碱基数/G Clean_bases	测序错误率/% Error_rate	Phred数值大于20的碱基百分比/% Q20	Phred数值大于30的碱基百分比/% Q30	过滤后reads中GC含量/% GC_pct
Y₇₃	0	1	3.08	0.0238	98.60	95.28	43.06
		2	4.41	0.0250	98.13	93.97	43.03
		3	3.63	0.0239	98.54	95.10	43.39
	4	1	2.28	0.0242	98.43	94.83	43.30
		2	3.75	0.0239	98.58	95.15	43.30
		3	5.14	0.0239	98.56	95.10	42.77
	8	1	3.54	0.0239	98.56	95.16	43.03
		2	3.84	0.0239	98.56	95.14	43.09
		3	3.73	0.0240	98.53	95.02	42.87
Y₅	0	1	3.97	0.0241	98.51	94.97	43.04
		2	3.15	0.0240	98.52	95.01	43.13
		3	4.26	0.0239	98.56	95.11	42.87
	4	1	5.63	0.0240	98.53	95.02	42.93
		2	4.89	0.0239	98.59	95.17	42.63
		3	5.93	0.0240	98.53	94.99	42.78
	8	1	5.52	0.0239	98.58	95.14	42.66
		2	4.42	0.0239	98.57	95.11	42.83
		3	4.44	0.0240	98.54	95.06	42.26
总计Total			75.61
平均Mean							42.94

样品 Sample	见光时间/h Light exposure time	重复 Repeat	过滤后碱基数/G Clean_bases	测序错误率/% Error_rate	Phred数值大于20的碱基百分比/% Q20	Phred数值大于30的碱基百分比/% Q30	过滤后reads中GC含量/% GC_pct
Y₇₃	0	1	3.08	0.0238	98.60	95.28	43.06
		2	4.41	0.0250	98.13	93.97	43.03
		3	3.63	0.0239	98.54	95.10	43.39
	4	1	2.28	0.0242	98.43	94.83	43.30
		2	3.75	0.0239	98.58	95.15	43.30
		3	5.14	0.0239	98.56	95.10	42.77
	8	1	3.54	0.0239	98.56	95.16	43.03
		2	3.84	0.0239	98.56	95.14	43.09
		3	3.73	0.0240	98.53	95.02	42.87
Y₅	0	1	3.97	0.0241	98.51	94.97	43.04
		2	3.15	0.0240	98.52	95.01	43.13
		3	4.26	0.0239	98.56	95.11	42.87
	4	1	5.63	0.0240	98.53	95.02	42.93
		2	4.89	0.0239	98.59	95.17	42.63
		3	5.93	0.0240	98.53	94.99	42.78
	8	1	5.52	0.0239	98.58	95.14	42.66
		2	4.42	0.0239	98.57	95.11	42.83
		3	4.44	0.0240	98.54	95.06	42.26
总计Total			75.61
平均Mean							42.94

样品 Sample	见光时间/h Light exposure time	重复 Repeat	有唯一比对位置的数量统计/% Unique_map	有多个比对位置的数量统计/% Multi_map	比对到两个外显子的数量统计/% Splice_map
Y₇₃	0	1	87.53	3.34	29.34
		2	88.44	3.70	32.32
		3	89.45	3.12	33.08
	4	1	85.85	2.93	28.61
		2	89.98	3.16	33.15
		3	89.76	3.21	31.95
	8	1	88.69	3.18	31.65
		2	87.88	3.41	31.60
		3	89.46	2.92	32.43
Y₅	0	1	89.72	3.15	31.67
		2	89.79	2.97	32.09
		3	89.02	3.24	31.20
	4	1	90.01	3.17	31.79
		2	89.74	3.13	31.07
		3	89.99	3.17	32.16
	8	1	89.82	3.08	32.31
		2	89.88	3.02	32.59
		3	88.85	3.12	30.48

样品 Sample	见光时间/h Light exposure time	重复 Repeat	有唯一比对位置的数量统计/% Unique_map	有多个比对位置的数量统计/% Multi_map	比对到两个外显子的数量统计/% Splice_map
Y₇₃	0	1	87.53	3.34	29.34
		2	88.44	3.70	32.32
		3	89.45	3.12	33.08
	4	1	85.85	2.93	28.61
		2	89.98	3.16	33.15
		3	89.76	3.21	31.95
	8	1	88.69	3.18	31.65
		2	87.88	3.41	31.60
		3	89.46	2.92	32.43
Y₅	0	1	89.72	3.15	31.67
		2	89.79	2.97	32.09
		3	89.02	3.24	31.20
	4	1	90.01	3.17	31.79
		2	89.74	3.13	31.07
		3	89.99	3.17	32.16
	8	1	89.82	3.08	32.31
		2	89.88	3.02	32.59
		3	88.85	3.12	30.48

见光时间/h Light exposure time	总数 All	上调 Up	下调 Down
0	2 545	1 060	1 485
4	4 578	2 179	2 399
8	4 689	2 038	2 651

Expression Analysis of Genes Related to Photosensitive Color Under the Caylx in Eggplant Based on Transcriptome Sequencing

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基因 Gene	基因所在的染色体支架 Scaffold	起始位置 Gene start	终止位置 Gene end	基因所在的正负链Gene strand	基因长度/bp Gene length	基因类型 Gene biotype	功能描述 Description
FAOMT	Sme2.5_00065.1	174 094	176 962	-	9 299	Protein_coding	Flavonoid 3',5'-methyltransferase
CCOAOMT	Sme2.5_00029.1	48 026	49 426	+	2 773	Protein_coding	Caffeoyl-CoA O-methyltransferase
CYP73A4	Sme2.5_00001.1	378 788	381 860	+	7 022	Protein_coding	Trans-cinnamate 4-monooxygenase
HST	Sme2.5_00662.1	75 387	76 427	-	8 649	Protein_coding	Shikimate O-hydroxycinnamoyl- transferase
CHI	Sme2.5_01193.1	60 036	65 871	+	8 137	Protein_coding	Chalcone--flavonone isomerase
ANS	Sme2.5_01638.1	20 424	22 517	-	9 652	Protein_coding	Leucoanthocyanidin dioxygenase
F3H	Sme2.5_00015.1	138 816	141 687	+	4 315	Protein_coding	Naringenin,2-oxoglutarate 3-dioxygenase（Fragment）
MYB113	Sme2.5_05099.1	10 820	12 177	-	3 325	Protein_coding	Transcription factor MYB113
PAL	Sme2.5_29581.1	346	879	+	8 772	Protein_coding	Phenylalanine ammonia-lyase 1

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