A Preliminary Study on UGTs Involving Glycosylation of Flavonoids Based on Transcriptome Analyses of Fortunella crassifolia Fruits

doi:10.16420/j.issn.0513-353x.2018-0774

ACTA HORTICULTURAE SINICA ›› 2019, Vol. 46 ›› Issue (4): 664-676.doi: 10.16420/j.issn.0513-353x.2018-0774

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A Preliminary Study on UGTs Involving Glycosylation of Flavonoids Based on Transcriptome Analyses of Fortunella crassifolia Fruits

TAN Yan1，MA Xiaodi1，WANG Jiuzhao1，LIU Xiaogang1,3，and ZENG Ming1,2,*

1College of Horticulture and Landscape Architecture，Southwest University，Chongqing 400716，China；2Key Laboratory of Horticulture Science for Southern Mountainous Regions，Ministry of Education，Chongqing 400715，China；3Key Laboratory of Horticultural Crop Germplasm Resources Utilization of Ministry of Agriculture，Xingcheng，Liaoning 125100，China

Online:2019-04-25 Published:2019-04-25

Abstract

Abstract: O- and C-glycoside flavonoids are important metabolites in Fortunella crassifolia plants. Glycosylation of flavonoids is catalyzed by UDP-glycosyltransferase（UGT）. In this study，we determined the flavonoid glycosides in kumquat fruits by using UPLC G2-S QTOF. A total of 11 flavonoid glycosides were detected，including C-glucoside，O-neohesperidoside，and C-neohesperidoside flavonoids. Subsequently，based on transcriptomes，we isolated 49 UGT genes with high expression levels in kumquat fruits. Further phylogenetic analysis showed that 13 UGTs of them might be involved in the glycosylation process of flavonoids. One UGT encoding C-glucosyltransferase catalyzed the C-glucosylation，9 UGTs encoding 7-O-glucosyltransferase were involved in the 7-O-glucosylation，and 3 UGTs encoding 1,2-rhamnosyltransferase，can perform rhamnosylation of flavonoids.

Key words: Fortunella crassifolia, flavonoids, UDP-glycosyltransferases gene, transcriptome analyses, Brassica rapa, clubroot disease, resistance breeding, gene mapping

CLC Number:

S 666

TAN Yan1，MA Xiaodi1，WANG Jiuzhao1，LIU Xiaogang1,3，and ZENG Ming1,2,*. A Preliminary Study on UGTs Involving Glycosylation of Flavonoids Based on Transcriptome Analyses of Fortunella crassifolia Fruits[J]. ACTA HORTICULTURAE SINICA, 2019, 46(4): 664-676.

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A Preliminary Study on UGTs Involving Glycosylation of Flavonoids Based on Transcriptome Analyses of Fortunella crassifolia Fruits

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