Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (7): 1359-1370.doi: 10.16420/j.issn.0513-353x.2021-0194

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Genetic Mapping for a Medium Short-fruit Mutant of Cucumber

CHENG Feng, SONG Mengfei, CAO Lei, ZHANG Mengru, YANG Zhige, CHEN Jinfeng, LOU Qunfeng*()   

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement,College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China
  • Received:2021-03-29 Revised:2021-05-06 Online:2021-07-25 Published:2021-08-10
  • Contact: LOU Qunfeng


We isolated a natural mutant,msf(medium short-fruit),from the cucumber inbred line CCMC,a North China type with long fruits. The mutant showed the phenotype of shorter fruit length and fruit neck length than the wild type and also showed short plant,abnormal development of lateral branches and fragile main stem. Through paraffin section and scanning electron microscope observation,it was found that the difference of mutant fruit length was caused by the change of cell size. Genetic analysis showed that the segregation ratio of wild-type phenotype long fruit and mutant phenotype medium short fruit in F2 segregation population conformed to Mendel’s 3︰1 genetic law. This indicated that msf mutation was a recessive trait controlled by a single gene. BSA-seq analysis showed that the candidate gene was located in a 26.7-30.9 Mb region of chromosome 1 of cucumber genome. The msf gene was mapped to a 28.4-29.8 Mb region using a F2 population containing 437 individuals from a cross between msf and ‘hazerd’(a European greenhouse-type inbred line with short fruit). CsaV3_1G044310 was identified as the candidate gene by genome-wide screening combined with dCAPS marker verification,which encodes a homologous protein of Arabidopsis type Ⅱ inositol polyphosphate 5-phosphatase(type Ⅱ 5 Ptase). The quantitative expression of gene and the determination of endogenous hormones auxin and gibberellin the mutant may cause the change of hormone levels and affect the growth and development of plant.

Key words: cucumber, fruit length, mutant, gene mapping, type Ⅱ inositol polyphosphate 5-phosphatase;

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