Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (7): 1317-1328.doi: 10.16420/j.issn.0513-353x.2020-0656

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Mapping and KASP Markers Development for Clubroot Resistance Gene BraA.Pb.8.4 in Chinese Cabbage

YANG Shuangjuan, Zhang Xiaowei, WEI Xiaochun, ZHAO Yanyan, WANG Zhiyong, ZHAO Xiaobin, LI Lin, YUAN Yuxiang**()   

  1. Institute of Horticulture,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China
  • Received:2020-12-24 Revised:2021-02-19 Online:2021-07-25 Published:2021-08-10
  • Contact: YUAN Yuxiang


In this study,Bulked Segregant Analysis Sequencing(BSA-seq)was conducted on two Chinese cabbage populations,named as 1E519BC1F2 and 4E511BC1F2. We identified a common candidate region from the above two populations,which located on 7.40-8.85 Mb of chromosome A08. Here,we named this region as BraA.Pb.8.4. BraA.Pb.8.4 might be a novel clubroot resistance gene for two reasons. Firstly,in terms of physical position,BraA.Pb.8.4 is distinct from previous reported genes,Crr1,Rcr9 and CRs. Secondly,markers linked to Crr1 and Rcr9 amplified the same product without length polymorphysims between resistant and susceptible materials,and only one of the four CRs linked markers (Probe60)produced polymorphysims. Among the candidate region of BraA.Pb.8.4,three KASP markers,BraA.Pb.8.4-K1,BraA.Pb.8.4-K2 and BraA.Pb.8.4-K3,were developed. These three KASP markers are co-dominant markers which can not only effectively separate homozygous resistant genotypes from the homozygous susceptible genotypes,but also distinguish the heterozygous genotypes. Marker BraA.Pb.8.4-K1 were further genotyped in a total of 257 individuals,which belongs to five populations. The results showed that the average consistency rate between genotypes and phynotypes of the above 257 individuals was high to 96.13%. Thus,the BraA.Pb.8.4 gene and the KASP markers developed here can be efficently used for marker-assisted selection(MAS)of clubroot resistant gene in breeding.

Key words: Chinese cabbage, clubroot, BraA.Pb.8.4 gene, gene mapping, KASP marker

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