Abstract:

The specific primers of Citrus chlorotic dwarf-associated virus（CCDaV）were designed from the conserved region of movement protein（MP） gene sequence which available in GenBank，and the reaction condition was optimized. A loop-mediated isothermal amplification（RT-LAMP）assay was established for detection of CCDaV. CCDaV was detected while Citrus tristeza virus（CTV），Citrus tatter leaf virus（CTLV），Citrus exocortis viroid（CEVd），Satsuma dwarf virus（SDV）and Huanglongbing（HLB）were not detected by the LAMP assay. Sensitivity of the LAMP assay was 100-fold higher than conventional PCR and same as real-time PCR. The positive rate of 50 samples from Yunnan，Sichuan and Chongqing by using the LAMP was 6%，same as conventional PCR and real-time PCR，suggesting the LAMP assay could detect CCDaV from field samples. The LAMP assay is a specific，sensitive and rapid method for detecting CCDaV.

Key words: Citrus, Citrus chlorotic dwarf-associated virus, LAMP, detection