Prediction of ORF for GAG of Ty1-copia Retrotransposons in Pear and Its Expression Analysis in Response to Light

doi:10.16420/j.issn.0513-353x.2016-0078

ACTA HORTICULTURAE SINICA ›› 2016, Vol. 43 ›› Issue (8): 1577-1584.doi: 10.16420/j.issn.0513-353x.2016-0078

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Prediction of ORF for GAG of Ty1-copia Retrotransposons in Pear and Its Expression Analysis in Response to Light

JIANG Shuang^1,2，LUO Jun²，SUN Yong-wang¹，CAI Dan-ying³，and TENG Yuan-wen^1,*

（¹The State Agricultural Ministry Laboratory of Horticultural Plant Growth，Development & Quality Improvement，Department of Horticulture，Zhejiang University，Hangzhou 310058，China；²Forestry and Fruit Tree Research Institute，Shanghai Academy of Agriculture Sciences，Shanghai 201403，China；³Institute of Horticulture，Zhejiang Academy of Agricultural Sciences，Hangzhou 310021，China）

Online:2016-08-25 Published:2016-08-25

Abstract

Abstract:

Different types of sequences of ORF for GAG in the whole genome of‘Dangshan Suli’（Pyrus pyrifolia White Pear Group）were predicted by bioinformatics methods. A total of 315 GAGsequences were obtained from Ty1-copia retrotransposons. The cluster analysis indicated that there were seven groups，among which，groups one and three had many conserved members. Sequence alignment of all GAG sequences in pear genome showed a high heterogeneity. A total of 34 members were expressed in bagging and bag-removed‘Meirensu’pear fruit，of which，six members（Ppgag16，Ppgag19，Ppgag23，Ppgag27，Ppgag29，and Ppgag30）were highly expressed. In bagging-treated fruit，some members of gag were expressed，suggesting that lightwas not a sole factor to activatethe expression of copia retrotransposons. In bag-removed fruit，the expression of two members（Ppgag5 and Ppgag23）were up-regulated，indicating that some members of the retrotransposons were regulated by light.

Key words: Pyrus, Ty1-copia, GAG, expression analysis

CLC Number:

S 661.2

JIANG Shuang，LUO Jun，SUN Yong-wang，CAI Dan-ying，and TENG Yuan-wen. Prediction of ORF for GAG of Ty1-copia Retrotransposons in Pear and Its Expression Analysis in Response to Light[J]. ACTA HORTICULTURAE SINICA, 2016, 43(8): 1577-1584.

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Prediction of ORF for GAG of Ty1-copia Retrotransposons in Pear and Its Expression Analysis in Response to Light

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