Expression of VvACO1 Gene in Grape and Genetic Transformation in Arabidopsis and Tomato

doi:10.16420/j.issn.0513-353x.2015-0569

ACTA HORTICULTURAE SINICA ›› 2016, Vol. 43 ›› Issue (12): 2315-2324.doi: 10.16420/j.issn.0513-353x.2015-0569

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Expression of VvACO1 Gene in Grape and Genetic Transformation in Arabidopsis and Tomato

TONG Shao-ming，XI Hai-xiu，AI Ke-jun，and HOU He-sheng*

（College of Life Sciences，Liaoning Normal University，Key Laboratory of Plant Biotechnology of Liaoning Province，Dalian，Liaoning 116081，China）

Online:2016-12-25 Published:2016-12-25

Abstract

Abstract:

ACC oxidase is one of the key rate-limiting enzymes which functioned in the biosynthesis of ethylene. An ACO gene designated as VvACO1 was cloned from leaves in grape by RT-PCR. The open reading frame of VvACO1 was 957 bp encoding 318 amino acids. The VvACO1 protein contained typical conserved domain with leucine zipper，Fe²⁺and ascorbate binding motif. The sequences of VvACO1 shared similarity of 87% and 84% with the ACOs from tobacco and tomato respectively. The transcriptional level of VvACO1 was different in different tissues or organs of grape and up-regulated by ethephon. Over-expression of VvACO1 in tomato showed earlier flowering and fruit ripening. Meanwhile，transgenic Arabidopsis showed that over-expression of VvACO1 caused decreased photosynthetic efficiency and Chl. a fluorescence intensity.

Key words: Vitis vinifera, VvACO1, expression analysis, tomato；Arabidopsis

CLC Number:

S 663.1

TONG Shao-ming，XI Hai-xiu，AI Ke-jun，and HOU He-sheng*. Expression of VvACO1 Gene in Grape and Genetic Transformation in Arabidopsis and Tomato[J]. ACTA HORTICULTURAE SINICA, 2016, 43(12): 2315-2324.

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Expression of VvACO1 Gene in Grape and Genetic Transformation in Arabidopsis and Tomato

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