Abstract: In this study，a fluorescent microsatellite-anchored fragment length polymorphism （MFLP） technique was established by adding universal M13 adapter to the anchored primers. Nine highly polymorphic primer pairs were selected from 360 selective amplification primer combinations，and were used to screen polymorphisms for 32 genotypes of flowering Chinese cabbage. The results showed that the polymorphic alleles for the nine primer pairs ranged from 10 to 31 in 32 flowering Chinese cabbage genotypes，with an average of 17.7 per primer combination. Polymorphism information content（PIC）ranged from 0.61 to 0.98 for nine primer combinations，with an average of 0.81. The genetic similarities were calculated and showed their distribution ranged from 0.277 to 0.836 among the 32 genotypes. By using polymorphic alleles data，the 32 flowering Chinese cabbage genotypes were clusteredinto 2 groups and 4 subgroups by UPGMA method. These results show that the developed fluorescent MFLP technique could effectively detect DNA polymorphisms in the flowering Chinese cabbage germplasm，indicating this technique is suitable for the fields of research and application of genetic analysis in flowering Chinese cabbage.

Key words: flowering Chinese cabbage, fluorescent MFLP, genetic diversity