Cloning and Expression Analysis of Sulfate Transporter CsSUL3.5 Gene in#br#
Tea Plant

doi:10.16420/j.issn.0513-353x.2015-0513

ACTA HORTICULTURAE SINICA ›› 2015, Vol. 42 ›› Issue (11): 2306-2314.doi: 10.16420/j.issn.0513-353x.2015-0513

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Cloning and Expression Analysis of Sulfate Transporter CsSUL3.5 Gene in#br# Tea Plant

HU Yu-rong1，YUE Chuan1，ZHOU Chao2，HUANG Yu-ting1，CAO Hong-li1，HAO Xin-yuan1，WANG#br# Xin-chao1，and ZENG Jian-ming1,*

1Tea Research Institute of the Chinese Academy of Agricultural Sciences，National Center for Tea Improvement/Key
Laboratory of Tea Biology and Resources Utilization，Hangzhou 310008，China；2College of Food Science，Southwest
University，Chongqing 400715，China

Online:2015-11-25 Published:2015-11-25

Abstract

Abstract:

The cDNA of putative sulfate transporter（CsSUL3.5）gene（GenBank accession number
KP984500）was cloned from tea plant[Camellia sinensis（L.）O. Kuntze]. Bioinformatics analysis
indicated that the cDNA of CsSUL3.5 containing 1 914 bp ORF encoded 637 amino acids residues with a
putative molecular mass of 70.38 kD. It was predicted that CsSUL3.5 was a non-secretory protein without
a signal peptide. CsSUL3.5 could be located in the plasma membrane with 11 transmembrane domains.
Further analyses showed that CsSUL3.5 had the typically conserved domain and the highest identity（74%）with Sulfate Transporter 3.5 of Nicotiana sylvestris. The real-time PCR analysis showed that
CsSUL3.5 transcripts were significantly increased upon the treatment of Na2SO4 and Na2SeO4.

Key words: tea plant, CsSUL3.5, selenium, cloning, expression analysis

CLC Number:

S 571.1

HU Yu-rong1，YUE Chuan1，ZHOU Chao2，HUANG Yu-ting1，CAO Hong-li1，HAO Xin-yuan1，WANG. Cloning and Expression Analysis of Sulfate Transporter CsSUL3.5 Gene in#br# Tea Plant[J]. ACTA HORTICULTURAE SINICA, 2015, 42(11): 2306-2314.

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Cloning and Expression Analysis of Sulfate Transporter CsSUL3.5 Gene in#br# Tea Plant

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