Physiological Characteristics of Selenium Uptake in Tea Plant Root

doi:10.16420/j.issn.0513-353x.2021-0784

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (9): 1967-1976.doi: 10.16420/j.issn.0513-353x.2021-0784

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Physiological Characteristics of Selenium Uptake in Tea Plant Root

GUO Lina¹, WANG Lu¹, HAO Xinyuan¹, QI Meng², LI Xiaoman¹^,³, WANG Xinchao¹, ZENG Jianming¹()

¹Key Laboratory of Tea Biology and Resources Utilization,Ministry of Agriculture and Rural Affairs,Tea Research Institute,Chinese Academy of Agricultural Sciences,Hangzhou 310008,China
²Ankang Research Center of Se-enriched Product,Ankang,Shaanxi 726000,China
³College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou 350002,China

Received:2022-05-25 Revised:2022-08-09 Online:2022-09-25 Published:2022-10-08

Abstract

Abstract:

The tea plants（Camellia sinensis）have a strong Se-enriching capacity,however,the physiological characteristics of Se uptake are still unclear. In this study,the effects of solution pH,cultivation time,Se concentrations and the energy metabolism/aquaporin/anion-channel inhibitors on selenate and selenite uptake in the roots of ‘Zhongcha 108’were investigated. The results of pH treatments found that the uptake rate of selenite decreased with the increase of pH. The uptake rate of selenate reached the highest at pH 3.0,and remained stable from pH 4.0 to pH 7.0,with no significant difference. Time-course analysis showed that there was a cumulative effect on the uptake of selenite and selenate by tea plant roots. The total Se content in roots increased significantly with the increase of cultivation time under selenite treatment,while showed a trend of increase first and then decreased under selenate treatment. Under different Se concentration treatments,there was a linear positive correlation between the uptake rate of Se and the solution Se concentration. The uptake rate of selenite was significantly higher than that of selenate. To investigate the pathways via which the selenate and selenite uptake of tea plant root,inhibitors treatments were carried out under pH 5.0 and 5 μmol · L^-1Se conditions. The results showed that both of selenate and selenite uptake of tea plant roots were inhibited significantly by the energy metabolism inhibitors of CCCP and DNP and the aquaporin inhibitor of AgNO₃,indicating that the uptake of selenate and selenite were energy-dependent and aquaporins-mediated processes. The uptake rate of selenite was inhibited by NPPB,however,the uptake rate of selenate was not affected by NPPB,indicating that the anion-channel was also the pathway for selenite uptake in tea plant root.

Key words: tea plant, selenate, selenite, pH, inhibitor, uptake rate

CLC Number:

S571.1

GUO Lina, WANG Lu, HAO Xinyuan, QI Meng, LI Xiaoman, WANG Xinchao, ZENG Jianming. Physiological Characteristics of Selenium Uptake in Tea Plant Root[J]. Acta Horticulturae Sinica, 2022, 49(9): 1967-1976.

Figures/Tables 4

Fig. 1 Uptake of selenite and selenate by roots of tea plants in response to different pH levels The statistical analyses of Na2SeO3 and Na2SeO4 treatments were independent,different small letters represent significant differences at 0.05 level. The same below.

Fig. 2 Accumulation of selenium by roots of tea plants at different times

Fig. 3 Uptake of selenite and selenate by roots of tea plants at various Se concentrations The error bars represent the standard deviation of four replicates.

Fig. 4 Uptake of selenium by roots of tea plants at energy metabolism inhibitors,the aquaporin inhibitor and anion-channel inhibitor Different small letters represent significant differences at 0.05 level.

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Physiological Characteristics of Selenium Uptake in Tea Plant Root

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