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ACTA HORTICULTURAE SINICA ›› 2014, Vol. 41 ›› Issue (6): 1191-1197.

• Research Notes • Previous Articles     Next Articles

Analysis of Genetic Diversity and Development of Fingerprinting Key Among Accessions of Kernel-using Apricot Using SRAP Markers

AI Peng-fei,SU Shan,and JIN Zhan-zhong*   

  1. (College of Biological Science and Engineering,Hebei University of Science and Technology,Shijiazhuang 050018,China)
  • Received:2013-12-27 Online:2014-06-25 Published:2014-06-25

Abstract: In order to evaluate the genetic relationship among accessions of kernel-using apricot,sequence related amplified polymorphism(SRAP)markers were used to evaluate genetic diversity of 24 samples. Fifteen primer pairs generated 280 bands,241 bands(85.34%)of which were polymorphic ones. SRAP primer pairs amplified 13 to 24 bands with an average of 18.7 bands per primer pair. Polymorphic information content(PIC)ranged from 0.28 to 0.65 with an average of 0.51. A fingerprinting key,established on the base of polymorphic bands amplified by a primer pair of Me4-Em4,could discriminate 24 accessions of kernel-using apricot. A dendrogram was constructed based on SRAP data using UPGMA culster method. The 24 accessions were classified into 4 major groups at the genetic similarity coefficient of 0.70,which was basically corresponded with the genetic relationship based on morphological traits.

Key words: kernel-using apricot, SRAP, genetic diversity, fingerprinting key

CLC Number: