Isolation of the Citrus CsTBL1 Gene Promoter and Its Activity in Transgenic Arabidopsis

ACTA HORTICULTURAE SINICA ›› 2014, Vol. 41 ›› Issue (5): 817-824.

Isolation of the Citrus CsTBL1 Gene Promoter and Its Activity in Transgenic Arabidopsis

MA Yan-yan1，CHEN Jiao1，WU Tian-li1，ZHANG Jun1，and ZHONG Guang-yan2,*

1College of Horticulture and Landscape Architecture，Southwest University/Citrus Research Institute，Chinese Academy of
Agricultural Sciences，Chongqing 400715，China；2Key Laboratory of South Subtropical Fruit Biology and Genetic
Resource Utilization，Ministry of Agriculture/Institution of Fruit Tree Research，Guangdong Academy of Agricultural
Sciences，Guangzhou 510640，China

Online:2014-05-25 Published:2014-05-25

Abstract

Abstract: A 2 361 bp long promoter of the CsTBL1 gene was isolated from sweet orange[Citrus
sinensis（L.）‘Olinda’]. Sequence analysis showed that the promoter harbors multiple stress-responsive
cis-elements. To gather clues of the specific functions of the promoter a promoter-reporter vector was
constructed，and introduced it into Arabidopsis by floral dip method. Analysis of T3 transgenic progenies
with histochemical staining showed that high GUS activity in whole plants of 1- and 3-day-old seedlings.
Strong GUS activity was still detected in cotyledons and roots of 7- and 20-day-old seedlings，but
disappeared in hypocotyls. In 50-day-old seedlings，however，GUS activity was only detected in root tips
and trichomes. GUS expression was induced by treatments with ethylene precursor ACC，ABA，MeJA，and SA，suggesting that CsTBL1 could be involved in plant development and stress responses.

Key words: citrus, promoter, GUS gene, CsTBL1 gene

CLC Number:

S 666

MA Yan-Yan, CHEN Jiao, WU Tian-Li, ZHANG Jun, ZHONG Guang-Yan. Isolation of the Citrus CsTBL1 Gene Promoter and Its Activity in Transgenic Arabidopsis[J]. ACTA HORTICULTURAE SINICA, 2014, 41(5): 817-824.

[1]	YE Zimao, SHEN Wanxia, LIU Mengyu, WANG Tong, ZHANG Xiaonan, YU Xin, LIU Xiaofeng, and ZHAO Xiaochun, . Effect of R2R3-MYB Transcription Factor CitMYB21 on Flavonoids Biosynthesis in Citrus [J]. Acta Horticulturae Sinica, 2023, 50(2): 250-264.
[2]	YU Tingting, LI Huan, NING Yuansheng, SONG Jianfei, PENG Lulin, JIA Junqi, ZHANG Weiwei, and YANG Hongqiang. Genome-wide Identification of GRAS Gene Family in Apple and Expression Analysis of Its Response to Auxin [J]. Acta Horticulturae Sinica, 2023, 50(2): 397-409.
[3]	JIANG Jingdong, WEI Zhuangmin, WANG Nan, ZHU Chenqiao, YE Junli, XIE Zongzhou, DENG Xiuxin, CHAI Lijun. Exploitation and Identification of Tetraploid Resources of Hongkong Kumquat（Fortunella hindsii） [J]. Acta Horticulturae Sinica, 2023, 50(1): 27-35.
[4]	YUAN Xin, XU Yunhe, ZHANG Yupei, SHAN Nan, CHEN Chuying, WAN Chunpeng, KAI Wenbin, ZHAI Xiawan, CHEN Jinyin, GAN Zengyu. Studies on AcAREB1 Regulating the Expression of AcGH3.1 During Postharvest Ripening of Kiwifruit [J]. Acta Horticulturae Sinica, 2023, 50(1): 53-64.
[5]	DU Yuling, YANG Fan, ZHAO Juan, LIU Shuqi, LONG Chaoan. Antifungal Mechanisms of Sodium New Houttuyfonate Against Penicillium digitatum [J]. Acta Horticulturae Sinica, 2023, 50(1): 145-152.
[6]	LI Zhenxi, PAN Ruixuan, XU Meirong, ZHENG Zheng, DENG Xiaoling. Development of Duplex Real-time PCR Assay of‘Candidatus Liberibacter asiatics’ [J]. Acta Horticulturae Sinica, 2023, 50(1): 188-196.
[7]	ZHU Kaijie, ZHANG Zhehui, CAO Lixin, XIANG Shunde, YE Junli, XIE Zongzhou, CHAI Lijun, and DENG Xiuxin, . A New Brown Late-ripening Navel Orange Cultivar‘Zongcheng’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 41-42.
[8]	ZHU Shiping, WEN Rongzhong, WANG Yuanyuan, and ZENG Yang. A New Very Late Ripening Citrus Variety‘Jinlegan’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 43-44.
[9]	ZHENG Lin, WANG Shuai, LIU Yunuo, DU Meixia, PENG Aihong, HE Yongrui, CHEN Shanchun, ZOU Xiuping. Gene Cloning and Expression Analysis of NAC Gene in Citrus in Response to Huanglongbing [J]. Acta Horticulturae Sinica, 2022, 49(7): 1441-1457.
[10]	YANG Haijian, ZHANG Yungui, ZHOU Xinzhi. A New Citrus Cultivar‘Yungui Cui Cheng’ [J]. Acta Horticulturae Sinica, 2022, 49(7): 1611-1612.
[11]	ZHANG Kai, MA Mingying, WANG Ping, LI Yi, JIN Yan, SHENG Ling, DENG Ziniu, MA Xianfeng. Identification of HSP20 Family Genes in Citrus and Their Expression in Pathogen Infection Responses Citrus Canker [J]. Acta Horticulturae Sinica, 2022, 49(6): 1213-1232.
[12]	LI Wenting, LI Cuixiao, LIN Xiaoqing, ZHENG Yongqin, ZHENG Zheng, DENG Xiaoling. Population Genetic Structure of Xanthomonas citri pv. citri in Guangdong Province Based on the STR Locus [J]. Acta Horticulturae Sinica, 2022, 49(6): 1233-1246.
[13]	MA Mingying, HAO Chenxing, ZHANG Kai, XIAO Guihua, SU Hanying, WEN Kang, DENG Ziniu, MA Xianfeng. CsSWEET2a Promotes the Infection of Xanthomonas citri subsp. citri [J]. Acta Horticulturae Sinica, 2022, 49(6): 1247-1260.
[14]	JIA Yamin, XU Hao, HU Wenlang, WANG Yuwen, YE Xin, CHEN Lisong, LI Yan, GUO Jiuxin. Magnesium Deficiency Altered in Iron Absorption,Subcellular Distribution,and Chemical Forms in Citrus Seedlings [J]. Acta Horticulturae Sinica, 2022, 49(5): 973-983.
[15]	WEI Zhuangmin, WEI Sijia, CHEN Peng, HU Jianbing, TANG Yuqing, YE Junli, LI Xianxin, DENG Xiuxin, CHAI Lijun. Identification of S-genotypes of 63 Pummelo Germplasm Resources [J]. Acta Horticulturae Sinica, 2022, 49(5): 1111-1120.

Isolation of the Citrus CsTBL1 Gene Promoter and Its Activity in Transgenic Arabidopsis

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments