Abstract: In order to establish a high-frequency regeneration system for rose，the crucial effects of light and plant growth regulators on somatic embryogenesis and plant regeneration in Rosa chinensis ‘Yueyuehong’were investigated using leaf as explant. The results showed that after 12 weeks of callus induction on embryo proliferation medium supplemented with 3.0 mg · L^-1 2,4-D + 0.5mg · L^-1 TDZ in the dark and a subculture on embryo mature medium under red light（with an intensity of 7.2 µmol · m^-2 · s^-1）treatment，somatic embryos with thick and expanded cotyledon（s），which had potential regenerating capability were mostly observed. While shoot-like embryos without cotyledon（s）were mostly observed in dark condition.

Key words: rose, red light, somatic embryogenesis, organogenesis