Sense Expression Vector Construction and Analysis of Transgenic Arabidopsis thaliana with CjAPL1 Gene from Camellia japonica

ACTA HORTICULTURAE SINICA ›› 2014, Vol. 41 ›› Issue (4): 789-796.

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Sense Expression Vector Construction and Analysis of Transgenic Arabidopsis thaliana with CjAPL1 Gene from Camellia japonica

SUN Ying-kun1,2，LI Ji-yuan1,*，and YIN Heng-fu1

（1Research Institute of Subtropical Forestry，Chinese Academy of Forestry，Fuyang，Zhejiang 311400，China；2College of Landscape Architecture and Forestry，Qingdao Agricultural University，Qingdao，Shandong 266109，China）

Received:2013-11-28 Online:2014-04-25 Published:2014-04-25

Abstract

Abstract: In order to investigate the gene function of CjAPL1，a homologof AP1 from Camellia japonica，during double flower formation，pCAMBIA1300-CjAPL1 sense expression vector was constructed. The restriction enzyme digestion result showed that CjAPL1 gene was correctly inserted into pCAMBIA1300 sites between 35S promoter and terminator，which indicated that an ectopic expression vector had been successfully constructed. The pCAMBIA1300-CjAPL1 construct was transformed into Arabidopsis thaliana by inflorescence soaking mediated with Agrobacterium，and 65 independent transgenic plants were obtained. Three randomly selected positive plants with obvious phenotypic changes were confirmed by PCR amplification and Southern blotting，and highexpression levels of CjAPL1 in transgenic plants were also detected by the Real time-PCR analysis. Compared with WT plants，obvious phenotypic changes including increases of 1 style and 1–4 stamens，sepals edge developed petaloid morphology with white characteristics，and early flowering，were observed. These results showed that overexpression ofCjAPL1gene could cause the change of floral organ morphology and number，and therefore indicated that CjAPL1could play notable functions during floral organ formation and double flower development.

Key words: Camellia japonica, CjAPL1, expression vector, transgenic expression

CLC Number:

S 685.14

SUN Ying-kun1,2，LI Ji-yuan1,*，and YIN Heng-fu1. Sense Expression Vector Construction and Analysis of Transgenic Arabidopsis thaliana with CjAPL1 Gene from Camellia japonica[J]. ACTA HORTICULTURAE SINICA, 2014, 41(4): 789-796.

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Sense Expression Vector Construction and Analysis of Transgenic Arabidopsis thaliana with CjAPL1 Gene from Camellia japonica

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