Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (3): 421-438.doi: 10.16420/j.issn.0513-353x.2020-0602
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JIA Bing, GUO Guoling, WANG Youyu, WEI Pengfei, YU Tao, CHANG Xiao, HENG Wei()
Received:
2020-09-28
Online:
2021-03-25
Published:
2021-04-02
Contact:
HENG Wei
E-mail:hengwei@ahau.edu.cn
CLC Number:
JIA Bing, GUO Guoling, WANG Youyu, WEI Pengfei, YU Tao, CHANG Xiao, HENG Wei. Relationship Between Morphogenesis and Carbohydrate Synthesis of the Calyx-Persistence Fruit and Calyx-shedding Fruit in Differentiation Stage of‘Dangshan Suli’Pear[J]. Acta Horticulturae Sinica, 2021, 48(3): 421-438.
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URL: https://www.ahs.ac.cn/EN/10.16420/j.issn.0513-353x.2020-0602
基因 Gene | ID | 引物序列 Sequence of primer | ||||
---|---|---|---|---|---|---|
F(5′-3′) | R(5′-3′) | |||||
PSⅠsubunitⅡ | Pbr008101.1 | CACAAGCCAGCCTCTTCACC | AAGATCGGCGAGGGAGTTG | |||
PSⅠsubunitⅣ | Pbr042856.1 | CCCAGCACCAACACCAGAA | CGACCGGGTAACGGGTATT | |||
PSⅠpsaK | Pbr021078.1 | GCTGCCACTGTGACCACTTCT | CCTGCCGTTGACTTCCTGTT | |||
F-typeATPase | Pbr031897.1 | GGATGCCGTGGATGATGAGT | GCTCATGGCGGTCATCCTAG | |||
PSⅡpsbD | Pbr002000.1 | CTTTCCTTGTGCCTATTTCGC | AGACCGCCTAATTGACACCAA | |||
Rubisco IV | Pbr005365.1 | GGCTTTCTGACCAGTCCTCG | GTACTGGAAGGACCGGGTGA | |||
Rubisco V | Pbr003848.1 | ATGGGGAGGAAAAGGTCAGG | TCCTGATGATGTCTGCAGCCT | |||
Rubisco VI | Pbr040261.1 | GCTTGGTTCGGTTACCCTCA | AAACCTGCTCACCTGGCTCA | |||
Cyt b6 f II | Pbr011265.1 | GTGCCAGGCAGCCAGTATTT | GCAACTTGACCAGCAGAGCC | |||
Chl a-b P4 | Pbr007291.1 | CAGACGGTGGCAAGACATCA | CAACATTGCCAGTCTCCCATT | |||
Chl a-b CP24 | Pbr000879.2 | TTCATCAATCCCGAGTGGCT | AGCAGTGAGCCGAAGGAGAA | |||
Chl a-b CP29.1 | Pbr021654.1 | CCAAGAAGCCCAAGAAAACAA | CCGTCGTAGTCGTACTGCAAAT |
Table 1 The primer sequences of the photosynthesis-related genes for qRT-PCR
基因 Gene | ID | 引物序列 Sequence of primer | ||||
---|---|---|---|---|---|---|
F(5′-3′) | R(5′-3′) | |||||
PSⅠsubunitⅡ | Pbr008101.1 | CACAAGCCAGCCTCTTCACC | AAGATCGGCGAGGGAGTTG | |||
PSⅠsubunitⅣ | Pbr042856.1 | CCCAGCACCAACACCAGAA | CGACCGGGTAACGGGTATT | |||
PSⅠpsaK | Pbr021078.1 | GCTGCCACTGTGACCACTTCT | CCTGCCGTTGACTTCCTGTT | |||
F-typeATPase | Pbr031897.1 | GGATGCCGTGGATGATGAGT | GCTCATGGCGGTCATCCTAG | |||
PSⅡpsbD | Pbr002000.1 | CTTTCCTTGTGCCTATTTCGC | AGACCGCCTAATTGACACCAA | |||
Rubisco IV | Pbr005365.1 | GGCTTTCTGACCAGTCCTCG | GTACTGGAAGGACCGGGTGA | |||
Rubisco V | Pbr003848.1 | ATGGGGAGGAAAAGGTCAGG | TCCTGATGATGTCTGCAGCCT | |||
Rubisco VI | Pbr040261.1 | GCTTGGTTCGGTTACCCTCA | AAACCTGCTCACCTGGCTCA | |||
Cyt b6 f II | Pbr011265.1 | GTGCCAGGCAGCCAGTATTT | GCAACTTGACCAGCAGAGCC | |||
Chl a-b P4 | Pbr007291.1 | CAGACGGTGGCAAGACATCA | CAACATTGCCAGTCTCCCATT | |||
Chl a-b CP24 | Pbr000879.2 | TTCATCAATCCCGAGTGGCT | AGCAGTGAGCCGAAGGAGAA | |||
Chl a-b CP29.1 | Pbr021654.1 | CCAAGAAGCCCAAGAAAACAA | CCGTCGTAGTCGTACTGCAAAT |
基因 Gene | ID | 引物序列 Sequence of primer | |
---|---|---|---|
F(5′-3′) | R(5′-3′) | ||
PpyS6PDH1 | Pbr042781.1 | AGACTGGACTTGTCAAGAGGGAA | GTCCTTGCCCAAAAGACTGG |
PpyS6PDH2 | Pbr023248.1 | AGCTTGGCTATCGCCATTTC | TTCCCTCTTGACAAGTCCAGTCT |
PpySDH1 | Pbr022043.1 | CAAAGGAGGCCGCTACAATC | CTAACATCGCCGGAGGAAAC |
PpySDH2 | Pbr032777.1 | CCTTCGGAGCACCAAGAATC | GGGACGAGTAGCATTGAGGC |
PpySDH3 | Pbr013915.1 | GCTGCTTGGCTCGTTGATG | ATCACCATCGGCTCTTTAACCT |
PpySOT2 | Pbr000331.1 | AAGGCAGTGGTGAGGTTGA | GCTCCAGACTTGTTACAGGCT |
PpySOT3 | Pbr005950.1 | AGCCACAGTTGGTCTCGGT | GCTTTGTCTGCCAACCAT |
PpySOT4 | Pbr013451.1 | TGTTGGTGACGAGAAAGAAGC | CACACTTGGTTGCCCTGTAAT |
PpySOT5 | Pbr014102.1 | CCGCACATAAACTGGAGGCT | AGCAGCCTTACTCATCTCCTCAA |
PpySOT8 | Pbr018463.1 | CAAGCAGAAAAAGAATCT | ATCTAATTAACTTGCCCT |
PpySOT10 | Pbr018465.1 | TATGGGGACAGCGGAACAGG | AAGCTCCTTCCTCTCGTCTCG |
PpySOT14 | Pbr018908.1 | GAATTGCTCCTTCATCCCACA | GCAGCTTGTGATCGTAGGAGG |
PpySOT15 | Pbr018910.1 | GTAGAACCTCCGACTGGATTGG | TGTGAGGAAGCCACGATATGAG |
PpySOT21 | Pbr032610.1 | AAGCACGATTGGAATGACGAT | ACGCAGCCTTAGAGGGAAGAT |
PpySOT25 | Pbr034759.1 | GCTGCTCTTTCTGTCGTATTTG | CCACTTGACCTCCTTTCTGAT |
PpySOT26 | Pbr037511.1 | CGTTGCTTCTTGCCAGC | CGTCCGTTGTTCTGACCA |
PpySOT29 | Pbr037515.1 | CTCGGCGACGCTAAGAAAGT | CAGTTCTTTCCATACGCCTTCA |
PpySOT30 | Pbr038546.1 | ACGGTGGCTGTTGGAGTTGT | GTGATGCACAACACGATGGC |
PpySOT31 | Pbr038547.1 | AACAGCAATGGTGGCGAGTAG | AACAGCAATGGTGGCGAGTAG |
PpySOT34 | Pbr039977.1 | TGATTGGTGCTGGATTGGTCT | GGTCTCCTCCCGAGTCTATCAA |
PpySOT35 | Pbr040466.1 | CTCGGCGACGCTAAGAAAGT | CAGTTCTTTCCATACGCCTTCA |
Table 2 The primer sequences of the sorbitol metabolism related genes for qRT-PCR
基因 Gene | ID | 引物序列 Sequence of primer | |
---|---|---|---|
F(5′-3′) | R(5′-3′) | ||
PpyS6PDH1 | Pbr042781.1 | AGACTGGACTTGTCAAGAGGGAA | GTCCTTGCCCAAAAGACTGG |
PpyS6PDH2 | Pbr023248.1 | AGCTTGGCTATCGCCATTTC | TTCCCTCTTGACAAGTCCAGTCT |
PpySDH1 | Pbr022043.1 | CAAAGGAGGCCGCTACAATC | CTAACATCGCCGGAGGAAAC |
PpySDH2 | Pbr032777.1 | CCTTCGGAGCACCAAGAATC | GGGACGAGTAGCATTGAGGC |
PpySDH3 | Pbr013915.1 | GCTGCTTGGCTCGTTGATG | ATCACCATCGGCTCTTTAACCT |
PpySOT2 | Pbr000331.1 | AAGGCAGTGGTGAGGTTGA | GCTCCAGACTTGTTACAGGCT |
PpySOT3 | Pbr005950.1 | AGCCACAGTTGGTCTCGGT | GCTTTGTCTGCCAACCAT |
PpySOT4 | Pbr013451.1 | TGTTGGTGACGAGAAAGAAGC | CACACTTGGTTGCCCTGTAAT |
PpySOT5 | Pbr014102.1 | CCGCACATAAACTGGAGGCT | AGCAGCCTTACTCATCTCCTCAA |
PpySOT8 | Pbr018463.1 | CAAGCAGAAAAAGAATCT | ATCTAATTAACTTGCCCT |
PpySOT10 | Pbr018465.1 | TATGGGGACAGCGGAACAGG | AAGCTCCTTCCTCTCGTCTCG |
PpySOT14 | Pbr018908.1 | GAATTGCTCCTTCATCCCACA | GCAGCTTGTGATCGTAGGAGG |
PpySOT15 | Pbr018910.1 | GTAGAACCTCCGACTGGATTGG | TGTGAGGAAGCCACGATATGAG |
PpySOT21 | Pbr032610.1 | AAGCACGATTGGAATGACGAT | ACGCAGCCTTAGAGGGAAGAT |
PpySOT25 | Pbr034759.1 | GCTGCTCTTTCTGTCGTATTTG | CCACTTGACCTCCTTTCTGAT |
PpySOT26 | Pbr037511.1 | CGTTGCTTCTTGCCAGC | CGTCCGTTGTTCTGACCA |
PpySOT29 | Pbr037515.1 | CTCGGCGACGCTAAGAAAGT | CAGTTCTTTCCATACGCCTTCA |
PpySOT30 | Pbr038546.1 | ACGGTGGCTGTTGGAGTTGT | GTGATGCACAACACGATGGC |
PpySOT31 | Pbr038547.1 | AACAGCAATGGTGGCGAGTAG | AACAGCAATGGTGGCGAGTAG |
PpySOT34 | Pbr039977.1 | TGATTGGTGCTGGATTGGTCT | GGTCTCCTCCCGAGTCTATCAA |
PpySOT35 | Pbr040466.1 | CTCGGCGACGCTAAGAAAGT | CAGTTCTTTCCATACGCCTTCA |
基因 Gene | ID | 引物序列Sequence of primer | ||
---|---|---|---|---|
F(5′-3′) | R(5′-3′) | |||
PbrSPS1 | Pbr003107.1 | ATGCCAAGTCGTCGTTGTTG | GTTCAAGACGACGTTTAGCCTTC | |
PbrSPS3 | Pbr009578.1 | GAAACGACTGGGTGAAC | GTGGAGATCGGTCTCAT | |
PbrSPS8 | Pbr042506.1 | ACCGGATGGAGATGGGGAT | CATAATCAATGTAAGGTTAGCAA | |
PbrSUS1 | Pbr001616.1 | CTGACCAATCCGTCTACTTTCCC | CTCAACGAGTCCTGTGAGGTTC | |
PbrSUS3 | Pbr003395.1 | ACCCGGGTCCAGAGCTTAC | AGCTTCCTGGGTGTGCTTCAAAAC | |
PbrSUS7 | Pbr012642.1 | CTGGAGGCAGTTGAACAAAGA | TGTGGCGATCTTACTCTTTACCT | |
PbrSUS12 | Pbr028302.1 | GCAAGGACCGACCTGGA | GAAAGTAGACGGATTGGTCAGCA | |
PbrSUS13 | Pbr031564.1 | CAGGCATGAAAGAAGCAAGGTC | GTAGCAGAAATGCCATCCACTG | |
PbrSUS15 | Pbr035996.1 | GTTGTGGCTGCGGGTGATCA | AGCCTCAATGACAGTCAAGCCG | |
PbrSUS17 | Pbr037395.1 | CTGACCCTCACCGGAGTTTATG | TGGGAAAACGTTCTCTTTCACTCCT | |
PpySUT1 | Pbr031281.1 | TGCTGGTGTTGCTTCTGGTG | GACAGAGTTTAACAAGAGGCCAAG | |
PpySUT2 | Pbr025968.1 | CGCCATTTCCCACAGTTCTAG | CTGTAGGGCCCAACCGAAT | |
PpySUT3 | Pbr039114.1 | TCGCTGATCTCACTGAAAAGGA | TCGCTGATCTCACTGAAAAGGA | |
PbTMT2 | Pbr023965.1 | TTGAATACCCTTCCGCAGTTTA | CATCGTGGAGACTCAGGCAAG | |
PbTMT3 | Pbr033292.1 | CAAGTTCTACAGAGGCTGCGTG | AAGAGTGCTTTGCCCAGTTACA | |
PbTMT4 | Pbr032130.1 | TTTGGCGGTTACCTTGGTTC | CCCAAGCATCAACCTCCAAC |
Table 3 The primer sequences of the sucrose metabolism related genes for qRT-PCR
基因 Gene | ID | 引物序列Sequence of primer | ||
---|---|---|---|---|
F(5′-3′) | R(5′-3′) | |||
PbrSPS1 | Pbr003107.1 | ATGCCAAGTCGTCGTTGTTG | GTTCAAGACGACGTTTAGCCTTC | |
PbrSPS3 | Pbr009578.1 | GAAACGACTGGGTGAAC | GTGGAGATCGGTCTCAT | |
PbrSPS8 | Pbr042506.1 | ACCGGATGGAGATGGGGAT | CATAATCAATGTAAGGTTAGCAA | |
PbrSUS1 | Pbr001616.1 | CTGACCAATCCGTCTACTTTCCC | CTCAACGAGTCCTGTGAGGTTC | |
PbrSUS3 | Pbr003395.1 | ACCCGGGTCCAGAGCTTAC | AGCTTCCTGGGTGTGCTTCAAAAC | |
PbrSUS7 | Pbr012642.1 | CTGGAGGCAGTTGAACAAAGA | TGTGGCGATCTTACTCTTTACCT | |
PbrSUS12 | Pbr028302.1 | GCAAGGACCGACCTGGA | GAAAGTAGACGGATTGGTCAGCA | |
PbrSUS13 | Pbr031564.1 | CAGGCATGAAAGAAGCAAGGTC | GTAGCAGAAATGCCATCCACTG | |
PbrSUS15 | Pbr035996.1 | GTTGTGGCTGCGGGTGATCA | AGCCTCAATGACAGTCAAGCCG | |
PbrSUS17 | Pbr037395.1 | CTGACCCTCACCGGAGTTTATG | TGGGAAAACGTTCTCTTTCACTCCT | |
PpySUT1 | Pbr031281.1 | TGCTGGTGTTGCTTCTGGTG | GACAGAGTTTAACAAGAGGCCAAG | |
PpySUT2 | Pbr025968.1 | CGCCATTTCCCACAGTTCTAG | CTGTAGGGCCCAACCGAAT | |
PpySUT3 | Pbr039114.1 | TCGCTGATCTCACTGAAAAGGA | TCGCTGATCTCACTGAAAAGGA | |
PbTMT2 | Pbr023965.1 | TTGAATACCCTTCCGCAGTTTA | CATCGTGGAGACTCAGGCAAG | |
PbTMT3 | Pbr033292.1 | CAAGTTCTACAGAGGCTGCGTG | AAGAGTGCTTTGCCCAGTTACA | |
PbTMT4 | Pbr032130.1 | TTTGGCGGTTACCTTGGTTC | CCCAAGCATCAACCTCCAAC |
Fig. 2 The relative expression comparison on the physiological index between the calyx-persistence fruit and calyx-shedding fruit of ‘Dangshan Suli’pear CPF. Calyx-persistence fruit;CSF. Calyx-shedding fruit;Different letters represent significant differences at the level of P ≤ 0.05.
Fig. 3 The relative expression comparison on the photosynthesis related genes between the calyx-persistence fruit and calyx-shedding fruit of‘Dangshan Suli’pear CPF. Calyx-persistence fruit;CSF. Calyx-shedding fruit;Different letters represent significant differences at the level of P ≤ 0.05.
Fig. 4 The relative expression comparison on sorbitol metabolism related genes between the calyx-persistence fruit and calyx-shedding fruit of‘Dangshan Suli’pear CPF. Calyx-persistence fruit;CSF. Calyx-shedding fruit;Different letters represent significant differences at the level of P ≤ 0.05.
Fig. 5 The relative expression comparison on sucrose metabolism related genes between the calyx-persistence fruit and calyx-shedding fruit of‘Dangshan Suli’pear CPF. Calyx-persistence fruit;CSF. Calyx-shedding fruit;Different letters represent significant differences at the level of P ≤ 0.05.
Fig. 6 The comparison on sorbitol and sucrose metabolism related enzyme activity between the calyx-persistence fruit and calyx-shedding fruit of‘Dangshan Suli’pear CPF. Calyx-persistence fruit;CSF. Calyx-shedding fruit;Different letters represent significant differences at the level of P ≤ 0.05.
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