Abstract: Phylogenetic tree was clustered by MEGA5.0 based on amino acid sequences of nine
Fragaria vesca inorganic phosphate transporters and 58 known phosphate transporters of other plants. The
results showed five inorganic phosphate transporters in Fragaria vesca were gathered the branch of
mycorrhizal phosphate transporter，so these five genes were preliminarily predicted candidates of
mycorrhizal phosphate transporter. Based on homologous sequences，full sequences of these five genes in
Fragaria × ananassa Duch.‘Benihoppe’were obtained. Real-time quantitative PCR showed that，FaPT4，
FaPT5 and FaPT8 genes were dramaticly induced in the mycorrhizal roots of strawberry combined with
cluster result above. Therefore，FaPT4，FaPT5 and FaPT8 were proven to be mycorrhizal phosphate
transporter genes in Fragaria × ananassa Duch.‘Benihoppe’.

Key words: Fragaria ×, ananassa, mycorrhizal phosphate transporter gene, gene cloning, phylogenetic tree, quantitative expression