CAPS and TSP Analyses of Grape SNP Markers

ACTA HORTICULTURAE SINICA ›› 2013, Vol. 40 ›› Issue (11): 2307-2315.

• Technologies and Methods • Previous Articles Next Articles

CAPS and TSP Analyses of Grape SNP Markers

GUO Da-long*，LI Meng，ZHANG Guo-hai*，and GUO Ming-xiao

College of Forestry，Henan University of Science & Technology，Luoyang，Henan 471003，China

Online:2013-11-25 Published:2013-11-25

Abstract

Abstract: The grape EST-SNP loci developed previously were firstly screened in order to establish
the ordinary analyzing method and investigate the performance of grape SNP markers. Twenty-eight CAPS
（cleaved amplified polymorphic sequences）primers and twenty-two TSP（temperature-switch PCR）
primers were designed to analyze the screened SNP loci. Finally，twenty CAPS primers and ten TSP
primers produced clear and polymorphic bands in 31 grape genotypes. The genotyping results showed that
all SNP loci have two alleles. The average polymorphism information content and Shannon index are
0.336 and 0.511，respectively. The genotyping and cluster results of this study showed that CAPS and TSP
method were efficient and reliable. The CAPS and TSP primers developed in this study could be used in
SNP genotyping，cultivar identification and genetic diversity analysis of grape varieties in future.

Key words: grape, SNP, CAPS, TSP

CLC Number:

S 663.1

GUO Da-Long, LI Meng, ZHANG Guo-Hai, GUO Ming-Xiao. CAPS and TSP Analyses of Grape SNP Markers[J]. ACTA HORTICULTURAE SINICA, 2013, 40(11): 2307-2315.

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CAPS and TSP Analyses of Grape SNP Markers

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