Phylogenetic Analysis and Identification of Dendrobium Species Based on Ribosomal DNA Internal Transcribed Spacer（ITS）Sequence

ACTA HORTICULTURAE SINICA ›› 2012, Vol. 39 ›› Issue (8): 1539-.

• Other Horticulture Plants • Previous Articles Next Articles

Phylogenetic Analysis and Identification of Dendrobium Species Based on Ribosomal DNA Internal Transcribed Spacer（ITS）Sequence

LI Dan, LI Zhen-Jian, MAO Ping, YAN Xue-Feng, CHUN Ze, MA Xin-Rong

（1Chengdu Institute of Biology，Chinese Academy of Sciences，Chengdu 610041，China；2Research Institute of Forestry，Chinese Academy of Forestry，Key Laboratory of Tree Breeding and Cultivation，State Forestry Administration，Beijing 100091，China）

Online:2012-08-25 Published:2012-08-25

Abstract

Abstract: Dendrobium is valued for its ornamental and medicinal purposes. However，the taxonomy of this genus is still in a state of confusion，and it is more difficult to authenticate after processing. Based on the emerging field of molecular systematics as a powerful classification tool，a phylogenetic analysis was conducted using sequences of the Internal Transcribed Spacer of nuclear ribosomal DNA（rDNA ITS） as DNA barcodes for species identification and phylogenetic analysis of Dendrobium plants. In this study， the ITS1-5.8S-ITS2 sequence database of the 43 Dendrobium samples was constructed. Among them，thirty-five fresh specimens were known and the other 8 processed dry samples were undetermined. The
results showed that four ITS sequences from Den. hainanense，Den. sinense and two Den. hybrida varieties of Den. Burana Charming and Den. Burana Stripe respectively were not promulgated in GenBank. Analyzing the relationship between ITS sequence discrepancies and the phenotypic characteristics revealed that the ITS homology was positively correlated to morphology traits. The phylogenetic trees were constructed based on the ITS sequences using Platanthera dilatata as an outgroup to root the tree. According to the analysis of phylogeny and cluster，the 35 Dendrobium known samples were divided into 5 clusters，and most of the samples（24 out of 35）were clustered together. Den. hancockii and Den. harveyanum were clustered together. Den. sinense Den. lindleyi and Den. jenkinsii were grouped to one branch. Den. capillipes was parted to a branch individually. Den. hainanense and Den. crumenatum were clustered together. The results displayed that the sections of most species divided by ITS were as the same as traditional classification. However，there were several species regrouped to different sections by ITS. And Den. salaccense was grouped to the same branch of Den. Burana Charming and Den. Burana Stripe， and the sections were not determined before. Moreover，the authentication of the eight unknown Dendrobium processed dry samples were also identified by rDNA ITS.

Key words: Dendrobium, ITS sequence, phylogenetic analysis, species identification

CLC Number:

S 682.31

LI Dan, LI Zhen-Jian, MAO Ping, YAN Xue-Feng, CHUN Ze, MA Xin-Rong. Phylogenetic Analysis and Identification of Dendrobium Species Based on Ribosomal DNA Internal Transcribed Spacer（ITS）Sequence[J]. ACTA HORTICULTURAE SINICA, 2012, 39(8): 1539-.

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Phylogenetic Analysis and Identification of Dendrobium Species Based on Ribosomal DNA Internal Transcribed Spacer（ITS）Sequence

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