Screening of Mutants Resistant to the Toxin Produced by Fusarium oxysporum f. sp. cubense Using Ethyl Methane Sulfonate and Toxin Mutagenesis Techniques

ACTA HORTICULTURAE SINICA ›› 2012, Vol. 39 ›› Issue (8): 1465-.

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Screening of Mutants Resistant to the Toxin Produced by Fusarium oxysporum f. sp. cubense Using Ethyl Methane Sulfonate and Toxin Mutagenesis Techniques

YANG Mei, SHU Can-Wei, CHEN Jian-Yi, NIE Jian-Ping, ZHOU Er-Xun

（College of Natural Resources and Environment，South China Agricultural University，Guangzhou 510642，China）

Online:2012-08-25 Published:2012-08-25

Abstract

Abstract: The calli and differentiation buds of banana were treated with chemical mutagen ethyl methane sulfonate（EMS）and the crude toxin produced by Fusarium oxysporum f. sp. cubense race 4（Foc 4），the causal agent of banana fusarium wilt，via a multi-step screen method. As a result，an optimized screen system was established and the resistant mutants were obtained. The optimized screen system as follows，Firstly，the calli and differentiation buds of banana were treated with 0.5%（v/v）of EMS for 40 minutes which resulted in the effect of“semi-lethal dose”；Then，the survived calli and differentiation buds of banana were screened directionally under the stress of successively increasing concentrations（5.0%→ 10.0%→12.5%）of Foc 4 crude toxin；And finally，toxin-resistant calli and toxin-resistant differentiation buds were successfully obtained by using this multi-step screen method. The regenerated banana plantlets derived from toxin-resistant mutants of differentiation buds were tested for their resistance to Foc 4 toxin， the results showed that the disease index（24.72）of mutant plantlets was significantly lower than that 96.11）of control plantlets，and the effect of toxin-resistance reached 74.28%.

Key words: banana, Musa spp., Fusarium oxysporum f. sp. cubense, toxin, ethyl methane sulfonate（EMS）, resistant mutants, in vitro screening

CLC Number:

YANG Mei, SHU Can-Wei, CHEN Jian-Yi, NIE Jian-Ping, ZHOU Er-Xun. Screening of Mutants Resistant to the Toxin Produced by Fusarium oxysporum f. sp. cubense Using Ethyl Methane Sulfonate and Toxin Mutagenesis Techniques[J]. ACTA HORTICULTURAE SINICA, 2012, 39(8): 1465-.

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Screening of Mutants Resistant to the Toxin Produced by Fusarium oxysporum f. sp. cubense Using Ethyl Methane Sulfonate and Toxin Mutagenesis Techniques

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