Abstract: The disease resistance identification，morphological characteristics，cytological identification and genetic relationships were analyzed using SSR markers for 4 materials of wild cucurbit. The results indicated that the 4 wild materialswere wild lvy gourd[Coccinia grandis（L.）Voigt]，wild bisexual cucumis（Cucumis bisexualis A. M. Lu et G. C. Wang ex Lu et Z. Y. Zhang），wild snakegourd （Trichosanthes L.），wild red-seed using watermelon（Citrullus lanatus ssp. vulgaris var. megalaspermusLin et Chao），respectively. The chromosome numbers of 4 wild materialswere 2n = 24，2n = 24，2n = 22，2n = 22，respectively. The wild characters of 4 wild materials were obvious，and some of the experimental species can’t be used directly because of their poor-fruit quality，but they showed the exuberant vitality. The wild lvy gourd，wild snakegourd，wild red-seed using watermelon were high resistant accessions of the two diseases，and the wild bisexual cucumis was susceptible accession of fusarium wilt and powdery mildew. 15 pairs of SSR primer selected from 130 pairs of primer generated total of 271 bands，124 （45.8%）of which were polymorphic through the analysis of the investigated accessions. The genetic similarity coefficient among 4 materials of wild cucurbit and 4 kinds of cultivated cucurbit materials including melon，cucumber，watermelon and pumpkin ranged from 0.62 to 0.96. The WINBOOT software showed that the wild lvy gourd，wild bisexual cucumis，wild snakegourd and wild red-seed using watermelon had narrow genetic relationship with cucumber，melon，pumpkin and watermelon，respectively.

Key words: wild cucurbit, morphological characteristics, disease resistance, chromosome, SSR marker, genetic relationship