Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (8): 1595-1604.doi: 10.16420/j.issn.0513-353x.2020-0730

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Preliminary Study on the Role of sly-miR166b and Its Target Genes in Tomato Resistance to Late Blight

ZHANG Xiaoyi, HONG Yuhui, ZHANG Yuanyuan, LUAN Yushi()   

  1. School of Bioengineering,Dalian University of Technology,Dalian,Liaoning 116024,China
  • Received:2020-09-03 Revised:2021-03-07 Online:2021-08-25 Published:2021-09-06
  • Contact: LUAN Yushi


To investigate the function of sly-miR166b in tomato resistance to late blight,the vectors that overexpressing and silencing sly-miR166b were constructed and transiently expressed in tomato plants respectively. The results showed that transient overexpressing sly-miR166b(TO)reduced its target genes expression. The ratio of lesion area to leaf area on TO leaves was much lower than that in control leaves upon inoculation. On the contrary,transient silencing sly-miR166b(TS)increased its target genes expressions. The ratio of lesion area to leaf area on TS leaves was much higher than that in control leaves upon inoculation. This study preliminarily showed that overexpression of sly-miR166b enhanced plant resistance to late blight,while silencing sly-miR166b enhanced plant susceptibility to late blight,laying a foundation for comprehensively revealing the sly-miR166b mechanism in disease resistance.

Key words: tomato, late blight, miRNA, target gene, disease resistance

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