Cloning and Characterization of Dehydrins Gene SlDHN2b in Tomato

ACTA HORTICULTURAE SINICA ›› 2012, Vol. 39 ›› Issue (10): 2015-2022.

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Cloning and Characterization of Dehydrins Gene SlDHN2b in Tomato

GUO Peng, ZHANG Shi-Gang, JIN Hua, ZOU Ji-Xiang, DONG Yan, JIANG Guo-Bin

（1College of Environment and Resources，Dalian Nationalities University，Dalian，Liaoning 116600，China；2Tai’an Municipal Bureau of Agriculture，Tai’an，Shandong 271018，China；3Liaoning Forestry Vocational and Technical College，Shenyang 110101，China）

Online:2012-10-25 Published:2012-10-25

Abstract

Abstract: In present study，a cDNA clone，designated SlDHN2b，was isolated from tomato by RT-PCR. The ORF of SlDHN2b was 1 140 bp and contained 380 amino acid residues. Protein sequence analysis showed that the SlDHN2b was high hydrophilic. Real-time PCR analysis indicated that mRNA accumulation of SlDHN2b was induced by salt stress，abscisic acid（ABA）and dehydration. Analysis of the promoter of PdEPF1 revealed the presence of stress-responsive elements by genome walking kit. Tissue-specific expression indicated that SlDHN2b was mainly expressed in leaves，weakly expressed in bud，flower，stem and the lowest in roots. Analysis of the promoter of SlDHN2b revealed the presence of stress-responsive elements.

Key words: tomato, SlDHN2b, cloning, abiotic stress, promoter

CLC Number:

S641.2

GUO Peng, ZHANG Shi-Gang, JIN Hua, ZOU Ji-Xiang, DONG Yan, JIANG Guo-Bin. Cloning and Characterization of Dehydrins Gene SlDHN2b in Tomato[J]. ACTA HORTICULTURAE SINICA, 2012, 39(10): 2015-2022.

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Cloning and Characterization of Dehydrins Gene SlDHN2b in Tomato

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