Characterization of Rh-ADF1 in Ethylene-regulated Petal Expansion in Roses

ACTA HORTICULTURAE SINICA ›› 2012, Vol. 39 ›› Issue (1): 119-126.

• Ornamental Plants • Previous Articles Next Articles

Characterization of Rh-ADF1 in Ethylene-regulated Petal Expansion in Roses

CHEN Xiang1，TIAN Ji1，YI Gen-fa2，PEI Hai-xia1，LI Jing1，CHEN Ji-wei1，MA Nan1，ZUO Zhi-rui3，and GAO Jun-ping1,*

（1Department of Ornamental Horticulture and Landscape Architecture，China Agricultural University，Beijing 100193，China；2Changping Municipal Bureau of Landscape and Forestry，Beijing 102200，China；3Engneering research center for floriculture，Zhangjiagang，Jiangsu 215617，China）

Received:2011-06-07 Revised:2011-12-20 Online:2012-01-25 Published:2012-01-25
Contact: GAO Jun-ping

Abstract

Abstract: In the present work，we isolated an ADF gene（actin depolymerizing factor）from petals of roses（Rosa hybrid‘Samantha’）and named it as Rh-ADF1 with accession NO. JN048105 and deposited in GenBank datebase. The cDNA full length of Rh-ADF1 contains an open reading frame with 423 bp in length，encoding a protein of 140 amino acids. Semi-quantitative RT-PCR analysis showed that expression level of Rh-ADF1 was slightly higher in petals than other four floral organs，including sepals，gynoecia，androecia，receptacles. The accumulation of Rh-ADF1 transcripts was strongly increased by ethylene in petals，and was obviously suppressed by 1-MCP，an action inhibitor of ethylene. Transient expression of GFP-Rh-ADF1 fusion gene in onion epidermal cells showed that Rh-ADF1 protein distributedubiquitously in the cell，while it preferentially located on filament networks of actins. Together，our results suggested that Rh-ADF1 may be involved in ethylene-regulated petal cell expansion at transcriptional level.

Key words: rose, cut flower, petal expansion, ethylene, Rh-ADF1, transcription regulation

CLC Number:

S 685.12

CHEN Xiang;TIAN Ji;YI Gen-fa;PEI Hai-xia;LI Jing;CHEN Ji-wei;MA Nan;ZUO Zhi-rui;and GAO Jun-ping;. Characterization of Rh-ADF1 in Ethylene-regulated Petal Expansion in Roses[J]. ACTA HORTICULTURAE SINICA, 2012, 39(1): 119-126.

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Characterization of Rh-ADF1 in Ethylene-regulated Petal Expansion in Roses

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