Colchicines Induced Polyploid Plants and Identification in Welsh Onion

ACTA HORTICULTURAE SINICA ›› 2011, Vol. 38 ›› Issue (12): 2381-2386.

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Colchicines Induced Polyploid Plants and Identification in Welsh Onion

DONG Fei1，CHEN Yun-qi2,*，LIU Shi-qi1，GAO Li-min2，WANG Chuan-zeng1，and CHEN Wei2

（1College of Horticulture Science and Engineering，Shandong Agricultural University，Tai’an，Shandong 271018，China；2Vegetable Research Institute of Shandong Academy of Agricultural Sciences/Key Laboratory for Biology of Greenhouse Vegetable of Shandong Province/National Improvement Center for Vegetable，Shandong Branch，Ji’nan 250100，China）

Received:2011-09-22 Revised:2011-11-29 Online:2011-12-25 Published:2011-12-25
Contact: CHEN Yun-qi

Abstract

Abstract: The welsh onion seeds were treated with colchicines at different concentration and treatment time duration，and the ploidy level was identified by morphological trait，chromosome number and flow cytometry techniques. The results showed that the best effect was obtained by the treatment of 0.8% colchicines with 48 hours. The mutated plants we got were vitreous seedlings，they could not survive after transplanting. By tissue culture we obtained tetraploids. The choromosome number of tetraploids was 2n = 4x = 32，the plants showed the general characteristics of tetraploids，such as darker leaf color，larger stomata，fewer stomata per unit area. Comparing the plant height and welsh onion stalk length，there were remarkable difference between tetraploids and the control.

Key words: welsh onion, colchicine, tetraploid induction, identification

CLC Number:

S 633.1

DONG Fei;CHEN Yun-qi;LIU Shi-qi;GAO Li-min;WANG Chuan-zeng;and CHEN Wei. Colchicines Induced Polyploid Plants and Identification in Welsh Onion [J]. ACTA HORTICULTURAE SINICA, 2011, 38(12): 2381-2386.

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Colchicines Induced Polyploid Plants and Identification in Welsh Onion

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