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ACTA HORTICULTURAE SINICA ›› 2011, Vol. 38 ›› Issue (1): 159-159–164 .

• Research Notes • Previous Articles     Next Articles

Establishment of SSR Fingerprinting on Autumn Cabbage Hybrids and Their Parents

CHEN Chen1,2,ZHANG Xing-tao3,CHENG Fei1,ZHUANG Mu2,*,LIU Yu-mei2,YANG Li-mei2,ZHANG Yang-yong2,and FANG Zhi-yuan2   

  1. (1Horticulture College of Qingdao Agricultural University,Qingdao,Shandong 266109,China;2Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;3Department of Life Science,College of Suzhou,Suzhou,Anhui 234000,China)
  • Received:2010-08-05 Revised:2010-11-23 Online:2011-01-25 Published:2011-01-25
  • Contact: ZHUANG Mu

Abstract: For the purpose of cabbage cultivar identification,SSR technology was used to establish fingerpringting for 18 cabbage accessions included six F1 hybrids(Jingfeng 1,Wanfeng,Zhonggan 8,Zhonggan 18,Zhonggan 19 and Zhonggan 22)and their parents. Based on the previous research on development of EST-SSR marker in cabbage,26 EST-SSR primer pairs were assessed validation of the amplification in above cabbage accessions. Used five primer pairs BoE974,BoE916,BoE337,BoE316 and BoE222,SSR fingerprint was obtained for six cabbage F1 hybrids and their parents,which was able to distinguish all of the 18 accessions. Moreover,Primer BoE222 was applied in purity identification of Jingfeng 1 hybrid,the result indicated that 90 of 99 were ture hybrid seeds,the purity was 90%.

Key words: cabbage, SSR marker, fingerprinting, purity identification

CLC Number: