Construction and Characterization of a Bacterial Artificial Chromosome Library from Chinese Cabbage

ACTA HORTICULTURAE SINICA ›› 2011, Vol. 38 ›› Issue (1): 151-151–158 .

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Construction and Characterization of a Bacterial Artificial Chromosome Library from Chinese Cabbage

FENG Da-ling1,2，SHI Xue-ping1，YANG Yu3，WANG Yan-hua1，XUAN Shu-xin1，ZHAO Jian-jun1，and SHEN Shu-xing1,*

（1College of Horticulture，Agricultural University of Hebei，Baoding，Hebei 071001，China；2College of Life Science，Agricultural University of Hebei，Baoding，Hebei 071001，China；3High-tech Research Center，Shandong Academy of Agricultural Sciences，Ji’nan 250100，China）

Received:2010-08-23 Revised:2010-12-20 Online:2011-01-25 Published:2011-01-25
Contact: SHEN Shu-xing

Abstract

Abstract: A bacterial artificial chromosome library of Brassica campestris L. ssp. pekinensis（Lour.）Olsson（Chinese cabbage）was constructed from inbred line‘85-1’with the vector pIndigoBAC-5. The key processes of the construction，such as preparation of high molecular weight DNA，selection of digested fragments，condition of ligation and transformation，were studied. The library consists of 57 600 clones in which the average insert size is about 98.4 kb and the empty clones are about 1.5%. The library represents an equivalent of 10.3 fold size of Chinese cabbage genome. Six clones randomly picked from this library show no HindⅢ fingerprint changes after 5 days’ successive culture，which indicates that the clones in the library are stable. The library will lay the foundation for gene clone，location and comparative genomics research of Brassica.

Key words: Chinese cabbage, bacterial artificial chromosome library, insert fragment, stability

CLC Number:

S 634.1

FENG Da-ling;SHI Xue-ping;YANG Yu;WANG Yan-hua;XUAN Shu-xin;ZHAO Jian-jun;and SHEN Shu-xing;. Construction and Characterization of a Bacterial Artificial Chromosome Library from Chinese Cabbage[J]. ACTA HORTICULTURAE SINICA, 2011, 38(1): 151-151–158 .

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Construction and Characterization of a Bacterial Artificial Chromosome Library from Chinese Cabbage

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