Abstract: An efficient plant regeneration protocol for Tagetes erecta L. was achieved via organogenesis from leaf explants in vitro. In this paper，the effects of different combinations of plant growth regulators，the addition of AgNO3，gel and the subculture medium on callus induction and buds regeneration were investigated. The research results indicated that the optimal media for buds regeneration is MS + 6-BA 3.0 mg · L-1 + IAA 3.0 mg · L-1 + sucrose 30.0 g · L-1 + phytagel 6.0 g · L-1 with the buds induction frequency of 97.5%. Put the 13-day-old explants with buds to the tissue culture flask，and 56.7% of them can grow up to 5 cm high after 30 days culture. The rooting of rootless shoots could be accomplished directly in the MS media with rooting frequency of 100%. All of the rooted shoots survived in greenhouse.

Key words: marigold, leaf, regeneration, plant growth regulator, adventitious bud