Abstract:

In order to analyze pistachio genetic relationship, random amplified polymorphic DNA (RAPD) was used to study the classification and identification of 13 pistachio (Pistacia vera L.）cultivars which includs 12 exotic varities and one native selects of Xinjiang. Twenty-one 10 bp primers that selected
from eighty arbitrary primers were applied for amplifying the pistachio DNA. Total 137 bands were produced, among which 122 bands (89.05%) were polymorphic. Genetic distances among the cultivars were estimated based on the amount of band sharing and ranged from 0.2015–0.5163, it is indicated that there are genetic differences among the cultivars. UPGMA cluster analysis showed that 13 pistachio varieties can be divided into three groups in the genetic distance of 0.40 level: The first category includes eleven varieties, the second category is the ‘Xinjiang selection’ and the third category is ‘Mateur’ varieties.

Key words: Pistacia vera L., genetic diversity, RAPD