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ACTA HORTICULTURAE SINICA ›› 2010, Vol. 37 ›› Issue (12): 1953-1953–1960.

• Vegetables • Previous Articles     Next Articles

Cloning and Expression of an OguCMS-related Anther-preferential Transcription Factor in Brassica oleracea

LIU Hai-xia1,2,KANG Jun-gen1,*,XIE Jian-ming2,JIAN Yuan-cai1,and DING Yun-hua1   

  1. (1Beijing Vegetable Research Center,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China;2College of Agronomy,Gansu Agricultural University,Lanzhou 730070,China)
  • Received:2010-08-18 Revised:2010-11-29 Online:2010-12-25 Published:2010-12-25
  • Contact: KANG Jun-gen

Abstract: A new anther preferential bHLH transcription factor was cloned using a down-regulated expression EST sequence as a querying probe. The gene as BoBHLH1(GenBank accession number:GU390530)was designated. BoBHLH1 is a new member of bHLH transcription factors family related to OguCMS and contains an 309 nt of continuous complete open reading frame(ORF)encoding a poly- peptide of 102 amino acids. BoBHLH1 is preferentially expressed in cabbage anther and have two expression peaks in early and late stages of anther development. The results provide new clues for further understanding of the nuclear-cytoplasmic interaction mechanism of OguCMS.

Key words: cabbage, OguCMS, cloning, bHLH transcription factor

CLC Number: