Regulatory Elements and Functional Analysis of Chalcone Synthase Gene Promoter from Ginkgo biloba L.

ACTA HORTICULTURAE SINICA ›› 2010, Vol. 37 ›› Issue (12): 1919-1919–1928.

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Regulatory Elements and Functional Analysis of Chalcone Synthase Gene Promoter from Ginkgo biloba L.

LI Lin-ling1,*，CHENG Hua1,*，CHENG Shui-yuan1,**，XU Feng2，WANG Yan2，and JIANG De-zhi2

（1College of Life Science and Engineering，Huanggang Normal University，Huanggang，Hubei 438000，China；2College of Horticulture and Gardening，Yangtze University，Jingzhou，Hubei 434025，China）

Received:2010-09-07 Revised:2010-12-04 Online:2010-12-25 Published:2010-12-25
Contact: LI Lin-ling

Abstract

Abstract: The regulative sequence（1 711 bp）of chalcone synthase gene promoter（CHSP）from Ginkgo biloba L. was cloned by genomic walking. In silico analysis suggested that the sequence contained several typical cis-acting elements，including UV/blue light responsive elements，phytohormone responsive elements，fungal elicitor responsive elements，MYB binding site，TATA-box and CAAT-box. A 1 402 bp promoter sequence upstream 5′ of translation start site of GbCHS was cloned and designated as GbCHSP，respectively. pBI121 + CHSP and pBI121-35S were constructed and transformed into tobaccos by LBA4404. These results showed that pBI121 and pBI121 + CHSP both could drive the transient expression of GUS in tobaccos and pBI121 + CHSP expressed differentially in root，stem and leaf tissues of tobacco. These results will be help to understand the transcriptional regulatory mechanism on GbCHS expression and flavonoids accumulation.

Key words: Ginkgo biloba L., chalcone synthase gene promoter, GUS, the analysis of cis-element

CLC Number:

S 664.3

LI Lin-ling;CHENG Hua;CHENG Shui-yuan;XU Feng;WANG Yan;and JIANG De-zhi. Regulatory Elements and Functional Analysis of Chalcone Synthase Gene Promoter from Ginkgo biloba L.[J]. ACTA HORTICULTURAE SINICA, 2010, 37(12): 1919-1919–1928.

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Regulatory Elements and Functional Analysis of Chalcone Synthase Gene Promoter from Ginkgo biloba L.

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