Cloning and Functional Analysis of SmDAD1 Promoter in Solanum melongena

doi:10.16420/j.issn.0513-353x.2019-0599

ACTA HORTICULTURAE SINICA ›› 2020, Vol. 47 ›› Issue (4): 643-652.doi: 10.16420/j.issn.0513-353x.2019-0599

• Research Papers • Previous Articles Next Articles

Cloning and Functional Analysis of SmDAD1 Promoter in Solanum melongena

ZHANG Shaowei1,*，YUAN Chao1,*，NIU Yi1,*，TANG Qinglin1，WEI Dayong1，WANG Yongqing2，TIAN Shibing2，YANG Yang2,**，and WANG Zhimin1,**

1Chongqing Key Laboratory of Olericulture，Key Laboratory of Horticulture Science for Southern Mountainous Regions Ministry of Education，College of Horticulture and Landscape Architecture，Southwest University，Chongqing 400715，China；2The Institute of Vegetables and Flowers，Chongqing Academy of Agricultural Sciences，Chongqing 400055，China

Online:2020-04-25 Published:2020-04-25

Abstract

Abstract: The promoter of SmDAD1 gene with 746 bp was cloned from eggplant accession‘F142’. Bioinformatics online software prediction showed that the promoter contained a plurality of cis-acting elements related to plant development and stress response. In order to further analyze the function of SmDAD1 promoter，prSmDAD1::GUS fusion expression vector was constructed. It was transferred into Arabidopsis thaliana，and GUS staining analysis was performed on T3 generation homozygous transgenic plant. The results showed that SmDAD1 promoter had very high activity in the roots，leaves and anthers，especially in the roots，but no activity in stems. Detection of GUS gene expression in transgenic plants indicated that SmDAD1 promoter was induced by MeJA，ABA，SA，GA，IAA and ACC. Therefore，we speculate that SmDAD1 may play important role in eggplant development and resistance to external stress.

Key words: Solanum melongena, promoter, SmDAD1, GUS gene, functional analysis

CLC Number:

S 641.1

ZHANG Shaowei1,*，YUAN Chao1,*，NIU Yi1,*，TANG Qinglin1，WEI Dayong1，WANG Yongqing2，TIAN Shibing2，YANG Yang2,**，and WANG Zhimin1,**. Cloning and Functional Analysis of SmDAD1 Promoter in Solanum melongena[J]. ACTA HORTICULTURAE SINICA, 2020, 47(4): 643-652.

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Cloning and Functional Analysis of SmDAD1 Promoter in Solanum melongena

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