Abstract: A system of complete plantlet regeneration directly from in vitro leaf of Chinese jujube ( Ziziphus jujuba Mill. ‘Dongzao’) was established after investgating proper culture conditions. Carbon source plays a key role on direct shoot inducement from in vitro leaf and maltose is the proper carbon source. AgNO3 could significantly improve the shoot regeneration rate and increase the shoot numbers. Under the medium of MS supplemented with TDZ 1.0 mg ·L - 1 + AgNO3 1.0 mg ·L - 1 , the highest shoot regeneration rate (97.3%) and shoot numbers (14.4) were obtained. But maltose was not suitable for the subsequent shoot growth. Regenerated shoots were growed and proliferated on medium of MS + sucrose 40 g·L - 1 + agar 4 g·L - 1 +BA 110 mg·L - 1 + IBA 0.5 mg·L - 1 with multiplication coefficient of 3.2. The complete plantlets were obtained after the shoots were rooted on MS medium plus IBA 110 mg·L -1 with rooting rate of 87.3%.

Key words: Ziziphus jujuba, leaf, in vitro, direct regeneration, adventitious shoot