The Identif ica tion and Sequence Analysis of 16S Ribosomal DNA of the Phytoplasma sp. Associated with Peach Yellow Disease

ACTA HORTICULTURAE SINICA ›› 2009, Vol. 36 ›› Issue (3): 341-346.

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The Identif ica tion and Sequence Analysis of 16S Ribosomal DNA of the Phytoplasma sp. Associated with Peach Yellow Disease

HUANG Yun^1*, WANG Jing¹,ZHAO Yan-qin²,and HE Miao¹

(¹Departm ent of Plant Pathology, S ichuan AgriculturalUniversity, Yapan, Sichuan 625014, China; ²Departm ent of Plant Protection, Inner Mongolia University for the N ationalities, Tongliao, InnerM ongolia 028043, China )

Received:2008-08-26 Revised:2008-12-15 Online:2009-03-25 Published:2009-03-25
Contact: HUANG Yun

Abstract

Abstract: The phytop lasma associated with peach yellow disease occurred in Longquanyi District in Sichuan Province was studied. The specific fluorescence dots for the pathogen were observed in phloem cells of leaf veins under a fluorescence microscope by staining with 4, 62diamidino222phenylindole diacetate. Phytoplasma-like particles were also found in the same tissues under a transmission electronic microscope. Its diameter was 40-650 nm, 350 nm on average and the thickness of the unitmembrane of the pathogen was 10 - 16 nm. The 16S rDNA about 115 kb was amplified by nested-PCR with universal p rimers for Phytoplasm a using the total DNA extracted from diseased peach as the temp lates. A phylogenetic tree was constructed based on the sequences of 16S rDNA. The result showed that Phytoplasm a associated with peach yellow disease was closely related to nectarine yellows (NecY2In1).

Key words: peach, peach yellow disease, Phytoplasma, morphology, phylogenetics, 16S rDNA

CLC Number:

S 662.11

HUANG Yun;WANG Jing;ZHAO Yan-qin;and HE Miao. The Identif ica tion and Sequence Analysis of 16S Ribosomal DNA of the Phytoplasma sp. Associated with Peach Yellow Disease[J]. ACTA HORTICULTURAE SINICA, 2009, 36(3): 341-346.

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The Identif ica tion and Sequence Analysis of 16S Ribosomal DNA of the Phytoplasma sp. Associated with Peach Yellow Disease

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