In vitro Study on the Interactions between Determinant Factors of Self-Incompatibility in Brassica oleracea L. var.capitata L.

ACTA HORTICULTURAE SINICA ›› 2009, Vol. 36 ›› Issue (3): 355-362.

• 蔬菜 • Previous Articles Next Articles

In vitro Study on the Interactions between Determinant Factors of Self-Incompatibility in Brassica oleracea L. var.capitata L.

YANG Yang¹, GAO Qi-Guo¹,SONG Ming¹, NIU Yi¹, TANG Qing-lin¹, ZHU Li-Quan²*, and WANG Xiao-Jia¹*

(¹Key Laboratory in Olericulture of Chongqing, Southwest University, Chongqing 400716, China; ²Plant Physiology and Biochemistry Laboratory of Southwest University, Chongqing 400716, China)

Received:2008-10-14 Revised:2009-01-20 Online:2009-03-25 Published:2009-03-25
Contact: ZHU Li-Quan;WANG Xiao-Jia

Abstract

Abstract: Self-incompatibility of Brassica oleracea L. var. capitata L. is determined by the female determinant S-locus receptor kinase(SRK) and the male determinant S-locus cysteine rich protein/S-locus protein 11(SCR/SP11). For further study on mechanism and reagent control of the interaction between the determinant factors, extracellular and transmember domains of SRK(named as mSRK) and open reading frame of SCR were amplified from Brassica oleracea L. cv. ZQ. Then fusion protein of mSRK about 116 kD and SCR-orf about 74 kD were expressed in E. coli BL21 by the pET·NusA fusion expression system and separated by SDS-PAGE electrophoresis. After SCR-orf was incubated with mSRK for 2 hours, the interaction between the two proteins was analyzed; the result shows that mSRK and SCR could combine with each other and form a stable complex.

Key words: Brassica oleracea L. var. capitata L., self-incompatibility, S-locus receptor kinase(SRK), S-locus cysteine-rich protein(SCR), prokaryotic expression

CLC Number:

S 635.1

YANG Yang;GAO Qi-Guo;SONG Ming;NIU Yi;TANG Qing-lin;ZHU Li-Quan;and WANG Xiao-Jia. In vitro Study on the Interactions between Determinant Factors of Self-Incompatibility in Brassica oleracea L. var.capitata L.[J]. ACTA HORTICULTURAE SINICA, 2009, 36(3): 355-362.

[1]	REN Fei, LU Miaomiao, LIU Jixiang, CHEN Xinli, LIU Daofeng, SUI Shunzhao, and MA Jing. Expression and Adversity Resistance Analysis of a Late Embryogenesis Abundant Protein Gene CpLEA from Chimonanthus praecox [J]. Acta Horticulturae Sinica, 2023, 50(2): 359-370.
[2]	WEI Zhuangmin, WEI Sijia, CHEN Peng, HU Jianbing, TANG Yuqing, YE Junli, LI Xianxin, DENG Xiuxin, CHAI Lijun. Identification of S-genotypes of 63 Pummelo Germplasm Resources [J]. Acta Horticulturae Sinica, 2022, 49(5): 1111-1120.
[3]	YU Qinhan, JIAO Shuzhen, WU Nan, ZHANG Ningbo, XU Weirong. Molecular Cloning,Expression and Polyclonal Antibody Preparation of E3 Ubiquitin Ligase Gene HOS1 from Vitis vinifera [J]. Acta Horticulturae Sinica, 2021, 48(6): 1173-1180.
[4]	HE Min, GU Chao, WU Juyou, ZHANG Shaoling. Recent Advances on Self-incompatibility Mechanism in Fruit Trees [J]. Acta Horticulturae Sinica, 2021, 48(4): 759-777.
[5]	HU Hao, YANG Ting, GAO Liping, Maarten A. Jongsma, WANG Caiyun. Cloning and Characterization of Key Synthase FAS Gene Involved in Terpenoids Pathway of Chrysanthemum morifolium [J]. Acta Horticulturae Sinica, 2021, 48(2): 313-324.
[6]	ZOU Hui, ZHAO Liang, YUAN Ting, GUO Qigao, WU Di, LIANG Guolu. Cloning and Expression of a Pathogenesis Related Protein Gene EjPR1 from Loquat Induced by Colletotrichum gloeosporioides [J]. Acta Horticulturae Sinica, 2021, 48(2): 367-376.
[7]	ZHOU Tao1,2，WANG Juan2，WANG Lulu1，WANG Baike2，HU Jiahui1,2，LAN Haiyan1,，and YU Qinghui2,. Cloning and Prokaryotic Expression Analysis of a Transcription Factor Gene SlWRKY16 in Tomato [J]. ACTA HORTICULTURAE SINICA, 2020, 47(7): 1312-1322.
[8]	LI Guihua1，ZHANG Tianyu2，TANG Xiaobing3，CHEN Hancai1,*，LI Tingyao1，and ZHANG Yan1 . A New Chinese Kale Cultivar‘Zitai Jielan’ [J]. ACTA HORTICULTURAE SINICA, 2019, 46(S2): 2777-2778.
[9]	LI Guihua1，CHEN Hancai1,*，ZHANG Yan1，LI Tingyao1，and CHEN Muxi2. A New Chinese Kale Cultivar‘Dongqiang Jielan’ [J]. ACTA HORTICULTURAE SINICA, 2019, 46(S2): 2779-2780.
[10]	GAO Qiguo1,，LEI Zhenze1,，LIANG Yunfei1,*，JIANG Yupeng1，and ZHU Liquan2. Screening of Brassica oleracea MLPK-interacting PUB Proteins Based on Gene Family Analysis [J]. ACTA HORTICULTURAE SINICA, 2019, 46(4): 714-722.
[11]	YANG Dan1,3,，LI Qing1,，ZHU Liquan3，ZHAO Tiantian1,2，LIANG Lisong1,2，WANG Guixi1,2，and MA Qinghua1,2,**. Cloning and Expression Analysis of Calmodulin Gene in Ping’ou Hybrid Hazelnut（Corylus heterophylla × C. avellana） [J]. ACTA HORTICULTURAE SINICA, 2018, 45(7): 1347-1358.
[12]	WANG Yicheng，WANG Nan，XU Haifeng，ZHANG Zongying，JIANG Shenghui，FANG Hongcheng，ZHANG Jing，SU Mengyu，XU Lin，and CHEN Xuesen*. Expression of a Cold-induced Gene MdICE1 in Red Flesh Apple and Its Protein Interaction with MdMYB [J]. ACTA HORTICULTURAE SINICA, 2018, 45(5): 817-826.
[13]	DING Tiyu1,，WU Mengmeng1,，ZHANG Ruiping1,，YAN Zhenli1,，YAN Yaru2，CHEN Dixin2，ZHANG Hengtao1，and GAO Qiming1. Identification of S-genotypes of 20 Apple Cultivars [J]. ACTA HORTICULTURAE SINICA, 2018, 45(12): 2277-2290.
[14]	PU Min1，LIAN Xiaoping2,，LUO Shaolan1，ZHANG Hecui1，WANG Yukui1，BAI Xiaojing1，ZENG Jing1，GAO Qiguo2，REN Xuesong2，and ZHU Liquan1,*. Transcriptome Analysis of Stigma After Pollination in Brassica oleracea var. capitata [J]. ACTA HORTICULTURAE SINICA, 2018, 45(1): 71-84.
[15]	LI Guihua，CHEN Hancai*，ZHANG Yan，and LI Tingyao. A New Chinese Cabbage Cultivar‘Qiuguan’ [J]. ACTA HORTICULTURAE SINICA, 2017, 44(s2): 2673-2674.

In vitro Study on the Interactions between Determinant Factors of Self-Incompatibility in Brassica oleracea L. var.capitata L.

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments